Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (2): 111-118.DOI: 10.13304/j.nykjdb.2021.0912
• ANIMAL AND PLANT HEALTH • Previous Articles
Tongtong ZHENG(), Wendi YANG, Ning WANG, Junjie MA, Long LIU, Qingyuan GUO(
)
Received:
2021-10-26
Accepted:
2022-02-07
Online:
2023-02-15
Published:
2023-05-17
Contact:
Qingyuan GUO
郑童童(), 杨雯迪, 王宁, 马俊杰, 刘龙, 郭庆元(
)
通讯作者:
郭庆元
作者简介:
郑童童 E-mail:2692104516@qq.com;
基金资助:
CLC Number:
Tongtong ZHENG, Wendi YANG, Ning WANG, Junjie MA, Long LIU, Qingyuan GUO. Morphological and Polygenetic Identification of Pathogen of Wheat Leaf Blight[J]. Journal of Agricultural Science and Technology, 2023, 25(2): 111-118.
郑童童, 杨雯迪, 王宁, 马俊杰, 刘龙, 郭庆元. 小麦叶枯病病原菌的形态学与多基因系统学鉴定[J]. 中国农业科技导报, 2023, 25(2): 111-118.
位点名称 Locus name | 引物名称 Primer name | 引物序列 Prime sequence (5’-3’) | 引物长度 Length of primer/bp | 来源 Origin |
---|---|---|---|---|
ITS | ITS1 | TCCGTAGGTGAACCTGCGG | 19 | [ |
ITS4 | TCCTCCGCTTATTGATATGC | 20 | ||
EF-1 | EF1-728F | CATCGAGAAGTTCGAGAAGG | 20 | [ |
EF1-986R | TACTTGAAGGAACCCTTACC | 20 | ||
Alt | Alt-F | ATGCAGTTCACCACCATCGC | 20 | [ |
Alt-R | ACGAGGGTGAYGTAGGCGTC | 20 |
Table 1 Primer sequence for PCR amplification
位点名称 Locus name | 引物名称 Primer name | 引物序列 Prime sequence (5’-3’) | 引物长度 Length of primer/bp | 来源 Origin |
---|---|---|---|---|
ITS | ITS1 | TCCGTAGGTGAACCTGCGG | 19 | [ |
ITS4 | TCCTCCGCTTATTGATATGC | 20 | ||
EF-1 | EF1-728F | CATCGAGAAGTTCGAGAAGG | 20 | [ |
EF1-986R | TACTTGAAGGAACCCTTACC | 20 | ||
Alt | Alt-F | ATGCAGTTCACCACCATCGC | 20 | [ |
Alt-R | ACGAGGGTGAYGTAGGCGTC | 20 |
Fig. 2 Some strains showed symptoms of inoculation in vitroA: Contrast; B: LK1 disease symptoms; C: LK3 disease symptoms; D: LK5 disease symptoms; E: LK19 disease symptoms; F: LK30 disease symptoms; G: YC3 disease symptoms; H: YC13 disease symptoms
Fig. 3 Some strains showed symptoms of leaf inoculation in roomA: Contrast; B: LK1 disease symptoms; C: LK3 disease symptoms; D: LK5 disease symptoms; E: LK19 disease symptoms; F: LK30 disease symptoms; G: YC3 disease symptoms; H: YC13 disease symptoms
Fig. 4 Strain YC3 characters and morphological characteristicsA: Positive morphology of colony; B: Dorsal morphology of colony; C: Conidium; D: Chain of conidia
Fig. 5 Strain LK19 characters and morphological characteristicsA: Positive morphology of colony; B: Dorsal morphology of colony; C: Conidium; D: Chain of conidia
Fig. 6 Strain LK3 characters and morphological characteristicsA: Positive morphology of colony; B: Dorsal morphology of colony; C: Conidium; D: Chain of conidia
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