Transcriptome Analysis of Resistance to Sharp Eyespot of Wheat Mediated by Piriformospora indica and Key Genes Screening

doi:10.13304/j.nykjdb.2023.0152

Abstract

Abstract:

Sharp eyespot of wheat is a frequent soil borne disease worldwide， which has serious harm on stable and high yield of wheat. To clarify the induction effect of Piriformospora indica， an endophytic fungus， on wheat resistance to sharp eyespot and to use a biological method which breaking through the traditional chemical control mode to enhance wheat resistance to sharp eyespot， wheat seeds were soaked in P. indica spore solution， followed by inoculation with Rhizoctonia cerealis， and physiological and biochemical indicators and transcriptome results were analyzed. The results showed that pre-colonization of P. indica in wheat could enhance the activity of antioxidant enzymes， alleviate the reduction of relative water content caused by Rhizoctonia cerealis and increase the membrane stability that partially destroyed by the fungi by 39.6%， and could also raise the content of chlorophyll by 32.3%， improving the photosynthesis ability of wheat. The transcriptome analysis revealed that the colonization of P. indica could restore the transcriptome changes caused by pathogenic fungi， induce the expression of related resistance genes， and improved plant biomass by influencing hormonal metabolic pathways. The resistance of wheat to R. cerealis had been comprehensively promoted by various ways and means. Key genes including TraesCS1A02G372300 and TraesCS1B02G393100 etc had been screened based on the transcriptome data， which would provide theoretical and experimental basis for catching on the kernal layer of plant-microbe interaction and acceleration of the breeding work related to disease resistance and yield increase.

Key words: Piriformospora indica, wheat, sharp eyespot, transcriptome analysis

摘要：

小麦纹枯病是一种世界性频发土传病害，对小麦稳产高产危害严重。为了明确内生真菌印度梨形孢（Piriformospora indica）对小麦抗纹枯病的诱导作用，突破传统依赖于化学药剂的防治模式，利用生物方法提高小麦对纹枯病的抗性，采用印度梨形孢孢子液浸泡小麦种子，然后用禾谷丝核菌（Rhizoctonia cerealis）侵染小麦植株，并对其相关生理生化指标和转录组数据进行分析。结果表明，小麦预先定殖印度梨形孢能增加细胞内抗氧化酶活性，缓解由于病菌引起的细胞内相对水含量降低，使因病菌侵染而破环的的膜稳定性上升39.6%，并能提高32.3%的叶绿素含量，增强小麦光合作用。转录组分析结果显示，印度梨形孢的定殖能改变病原菌引起的转录组变化并诱导相关抗性基因表达，通过影响激素代谢途径，提高植物生物量，利用多种路径和手段综合提高小麦对禾谷丝核菌的抗性。基于转录组数据筛选出了TraesCS1A02G372300、TraesCS1B02G393100等关键基因，为深入理解植物与微生物互作、加速相关抗病增产育种工作等提供理论基础与试验依据。

关键词: 印度梨形孢, 小麦, 纹枯病, 转录组分析

CLC Number:

S435.121

Shuo SHI, Yu FENG, Liang LI, Rui MENG, Yanze ZHANG, Xiurong YANG. Transcriptome Analysis of Resistance to Sharp Eyespot of Wheat Mediated by Piriformospora indica and Key Genes Screening[J]. Journal of Agricultural Science and Technology, 2025, 27(5): 133-145.

史硕, 冯宇, 李亮, 孟瑞, 章延泽, 杨秀荣. 印度梨形孢介导小麦抗纹枯病的转录组分析及关键基因筛选[J]. 中国农业科技导报, 2025, 27(5): 133-145.

Figures/Tables 10

Table 1 Respective treatments for different sample

处理 Treatment	重复 Repeat	总干净片段 Total clean read/10⁶	对比到基因组的总比对率 Total mapping genome ratio/%	对比到基因组的唯一比对率 Uniquely mapping genome ratio/%
Mock	3	88.57	75.83	19.69
Piri	3	88.94	75.07	20.03
Rh	3	88.31	78.25	18.85
Piri+ Rh	3	88.64	77.87	19.93

Fig. 1 Identification of P. indica colonization in wheat rootA： Control group soaking in Tween-20 solution； B： Treatment group soaking in P. indica

Fig. 2 Plant and its biomass after colonization of P. indicaA: Wheat leaves of Rh and Piri+Rh groups after 14 d of infection; B: Wheat plants of Rh and Piri+Rh groups after 60 d of continuous observation; C: Dry weight of 100 plants among treatment groups. The asterisks above the columns represent the level of difference between each treatment group and the control group, and the asterisks between the columns represent the level of difference between different treatment groups; ** and **** represent significance differences at P<0.01 and P<0.000 1 levels, respectively

Fig. 3 Disease index，leaf relative water content，membrane stability index and MDA content of wheat after colonization of P. indicaNote： The asterisks above the columns represent the level of difference between each treatment group and the control group, and the asterisks between the columns represent the level of difference between different treatment groups； ** ,*** and **** represents significance differences at P<0.01, P<0.001 and P<0.000 1 levels, respectively.

Fig. 4 Antioxidant enzymes activity in wheat seedlings after colonization of P. indica

Fig. 5 Chlorophyll content of leaves after inoculation of R.cerealis for 14 and 21 dNote：*** and **** represents significance differences between control group at P<0.001 and P<0.000 1 levels，respectively.

Fig. 6 Venn Diagram and Volcano Plot of different expressed genes among different treatment groupsA: Venn Diagram of the number of different expressed genes; B: Volcano Plot of different expressed genes in Rh and Mock group; C: Volcano Plot of different expressed genes in Piri and Mock group; D: Volcano Plot of different expressed genes in Piri+Rh and Mock group

Fig. 7 Enrichment Bubble Plot of signal pathway in different treatment groupsA: DEGs enriched in KEEG pathway in Rh and Mock groups; B: DEGs enriched in KEEG pathway in Piri + Rh and Mock groups

Fig. 8 Histogram of GO enrichment analysis for different treatment groupsA: DEGs enriched in GO pathway in Rh and Mock groups; B: DEGs enriched in GO pathway in Piri + Rh and Mock groups

Fig. 9 Relative expression of certain key genes by qPCRNote： * ， ** ，***and****indicate significance differences between control group at P<0.05， P<0.01， P<0.001 and P<0.000 1 levels，respectively.

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