Effects of Tartary Buckwheat Rotation on Enzyme Activities and Microorganisms in Rhizosphere Soil of Cultivated Potato in Yunnan Province

doi:10.13304/j.nykjdb.2023.0227

Abstract

Abstract:

In order to investigate the effects of continuous cropping and tartary buckwheat （KQ） rotation on rhizosphere soil enzyme activity， microbial quantity and yield of 3 main potato varieties in Yunnan province， the main potato varieties ‘Hezuo 88’ （HZ88）， ‘Lishu 6’ （LS6H） and ‘Qingshu 9’ （QS9H） were used as experimental materials. The activities of sucrase， protease， urease， cellulase， microbial quantity in rhizosphere soil and potato yield under different treatments were analyzed. The results showed that， compared with HZ88-HZ88， LS6H-LS6H and QS9H-QS9H treatments， the yields per potato plant of KQ-HZ88， KQ-LS6H and KQ-QS9H rotation treatments were significantly increased by 76.32%， 80.95% and 90.91%， respectively， the reducing sugar contents were significantly reduced by 38.71%， 18.42% and 21.43%， respectively. Compared with HZ88-HZ88-HZ88， LS6H-LS6H and QS9H-QS9H-QS9H treatments， the soil sucrase activities of HZ88-KQ-HZ88， KQ-HZ88-KQ， LS6H-KQ-LS6H， KQ-LS6H-KQ and QS9H-KQ-QS9H， KQ-QS9H-KQ rotation treatments were significantly increased by 8.54%， 14.00%， 22.73%， 34.25%， 18.13%， 25.12%， respectively， and the numbers of bacteria were significantly increased by 19.05%（P>0.05）， 92.86%， 75.68%， 72.97%， 67.11%， 73.21%， respectively. The changes of soil enzyme activity and microbial quantity might be one of the important reasons for potato continuous cropping obstacles in Yunnan region. Rotation could effectively improve rhizosphere soil enzyme activity， increase the number of beneficial bacteria in the soil， and alleviate continuous cropping obstacles.

Key words: potato, continuous cropping, rotation with tartary buckwheat, soil enzyme activity, soil microorganism

摘要：

为探讨连作和与苦荞（KQ）轮作对云南省3个马铃薯主栽品种根际土壤酶活性、微生物数量和产量的影响，以马铃薯主栽品种‘合作88’（HZ88）、‘丽薯6号’（LS6H）、‘青薯9号’（QS9H）为试验材料，对不同处理下根际土壤的蔗糖酶、蛋白酶、脲酶、纤维素酶活性和微生物数量以及马铃薯产量、品质进行分析。结果表明，KQ-HZ88、KQ-LS6H、KQ-QS9H轮作处理的马铃薯单株产量分别较HZ88-HZ88、LS6H-LS6H、QS9H-QS9H连作处理显著增加76.32%、80.95%、90.91%，还原糖含量显著降低38.71%、18.42%、21.43%。与3年连作相比，HZ88-KQ-HZ88和KQ-HZ88-KQ、LS6H-KQ-LS6H和KQ-LS6H-KQ、QS9H-KQ-QS9H和KQ-QS9H-KQ轮作处理的土壤蔗糖酶活性分别较HZ88-HZ88-HZ88、LS6H-LS6H-LS6H、QS9H-QS9H-QS9H连作处理显著增加8.54%和14.00%、22.73%和34.25%、18.13%和25.12%；细菌数量显著增加19.05%（P>0.05）和92.86%、75.68%和72.97%、67.11%和73.21%。土壤酶活性及微生物数量的变化可能是引起云南地区马铃薯连作障碍的重要原因之一，轮作可有效改善根际土壤酶活性，增加土壤中有益菌数量，缓解连作障碍。

关键词: 马铃薯, 连作, 苦荞轮作, 土壤酶活性, 土壤微生物

CLC Number:

S154.3

Yangyang DU, Yuanyuan BAO, Xiangyu LIU, Xinyong ZHANG. Effects of Tartary Buckwheat Rotation on Enzyme Activities and Microorganisms in Rhizosphere Soil of Cultivated Potato in Yunnan Province[J]. Journal of Agricultural Science and Technology, 2024, 26(5): 192-200.

杜洋洋, 包媛媛, 刘项宇, 张新永. 荞麦轮作对云南栽培马铃薯根际土壤酶活和微生物的影响[J]. 中国农业科技导报, 2024, 26(5): 192-200.

Figures/Tables 4

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指标 Index	年份 Year	处理Treatment
指标 Index	年份 Year	HZ88-HZ88-HZ88	LS6H-LS6H-LS6H	QS9H-QS9H-QS9H	KQ-KQ-KQ	HZ88-KQ-HZ88	LS6H-KQ-LS6H	QS9H-KQ-QS9H	KQ-HZ88-KQ	KQ-LS6H-KQ	KQ-QS9H-KQ
蔗糖酶活性 Sucrase activity/ （mg·g^-1）	2020	21.55 αbc	22.82 αabc	23.70 αa	21.78 αbc	21.09 βc	22.19 βabc	23.63 βa	22.86 αab	22.64 βabc	22.06 βabc
	2021	20.28 αc	20.08 βc	19.74 βc	23.93 αb	32.79 αa	32.83 αa	35.65 αa	20.17 βc	21.21 βbc	19.70 γc
	2022	20.72 αf	19.36 βg	21.62 αβef	22.92 αcd	22.49 βde	23.76 βc	25.54 βb	23.62 αcd	25.99 αab	27.05 αa
蛋白酶活性 Proteinase activity/ （µg·g^-1）	2020	40.17 αcd	46.43 αab	47.34 αa	37.24 βde	42.69 βbc	47.61 αa	50.20 αβa	37.81 βde	35.12 βe	37.58 γde
	2021	44.41 αab	29.73 βd	34.88 βc	41.14 αβb	40.44 βb	47.91 αa	45.62 βa	40.57 βb	34.65 βc	44.44 βab
	2022	45.05 αe	44.61 αe	46.70 αde	44.55 αe	49.80 αcd	51.04 αbc	54.55 αb	60.40 αa	40.47 αf	53.47 αbc
脲酶活性 Urease activity/ （mg·g^-1）	2020	0.20 βb	0.28 βa	0.19 βb	0.14 γc	0.20 γb	0.28 γa	0.19 γb	0.15 βc	0.15 βc	0.15 γc
	2021	0.21 βd	0.23 γc	0.17 βe	0.27 βb	0.31 βa	0.31 βa	0.30 βa	0.16 βe	0.16 βe	0.21 βd
	2022	0.39 αd	0.40 αcd	0.42 αcd	0.50 αa	0.40 αcd	0.42 αcd	0.43 αc	0.46 αb	0.42 αcd	0.41 αcd
纤维素酶活性 Cellulase activity/ （mg·g^-1）	2020	0.09 βbc	0.10 αab	0.08 βbc	0.10 βab	0.09 γbc	0.11 βa	0.08 γc	0.11 βa	0.11 αa	0.10 βab
	2021	0.13 αcd	0.09 αd	0.12 αcd	0.14 αc	0.24 αa	0.18 αb	0.25 αa	0.15 αbc	0.13 αc	0.12 βcd
	2022	0.06 γd	0.08 αd	0.11 αbc	0.16 αa	0.13 βb	0.12 βb	0.10 βc	0.11 βbc	0.12 αbc	0.16 αa

指标 Index	年份 Year	处理Treatment
指标 Index	年份 Year	HZ88-HZ88-HZ88	LS6H-LS6H-LS6H	QS9H-QS9H-QS9H	KQ-KQ-KQ	HZ88-KQ-HZ88	LS6H-KQ-LS6H	QS9H-KQ-QS9H	KQ-HZ88-KQ	KQ-LS6H-KQ	KQ-QS9H-KQ
蔗糖酶活性 Sucrase activity/ （mg·g^-1）	2020	21.55 αbc	22.82 αabc	23.70 αa	21.78 αbc	21.09 βc	22.19 βabc	23.63 βa	22.86 αab	22.64 βabc	22.06 βabc
	2021	20.28 αc	20.08 βc	19.74 βc	23.93 αb	32.79 αa	32.83 αa	35.65 αa	20.17 βc	21.21 βbc	19.70 γc
	2022	20.72 αf	19.36 βg	21.62 αβef	22.92 αcd	22.49 βde	23.76 βc	25.54 βb	23.62 αcd	25.99 αab	27.05 αa
蛋白酶活性 Proteinase activity/ （µg·g^-1）	2020	40.17 αcd	46.43 αab	47.34 αa	37.24 βde	42.69 βbc	47.61 αa	50.20 αβa	37.81 βde	35.12 βe	37.58 γde
	2021	44.41 αab	29.73 βd	34.88 βc	41.14 αβb	40.44 βb	47.91 αa	45.62 βa	40.57 βb	34.65 βc	44.44 βab
	2022	45.05 αe	44.61 αe	46.70 αde	44.55 αe	49.80 αcd	51.04 αbc	54.55 αb	60.40 αa	40.47 αf	53.47 αbc
脲酶活性 Urease activity/ （mg·g^-1）	2020	0.20 βb	0.28 βa	0.19 βb	0.14 γc	0.20 γb	0.28 γa	0.19 γb	0.15 βc	0.15 βc	0.15 γc
	2021	0.21 βd	0.23 γc	0.17 βe	0.27 βb	0.31 βa	0.31 βa	0.30 βa	0.16 βe	0.16 βe	0.21 βd
	2022	0.39 αd	0.40 αcd	0.42 αcd	0.50 αa	0.40 αcd	0.42 αcd	0.43 αc	0.46 αb	0.42 αcd	0.41 αcd
纤维素酶活性 Cellulase activity/ （mg·g^-1）	2020	0.09 βbc	0.10 αab	0.08 βbc	0.10 βab	0.09 γbc	0.11 βa	0.08 γc	0.11 βa	0.11 αa	0.10 βab
	2021	0.13 αcd	0.09 αd	0.12 αcd	0.14 αc	0.24 αa	0.18 αb	0.25 αa	0.15 αbc	0.13 αc	0.12 βcd
	2022	0.06 γd	0.08 αd	0.11 αbc	0.16 αa	0.13 βb	0.12 βb	0.10 βc	0.11 βbc	0.12 αbc	0.16 αa

指标 Index	年份 Year	处理Treatment
指标 Index	年份 Year	HZ88-HZ88-HZ88	LS6H-LS6H-LS6H	QS9H-QS9H-QS9H	KQ- KQ-KQ	HZ88-KQ-HZ88	LS6H-KQ-LS6H	QS9H-KQ-QS9H	KQ-HZ88-KQ	KQ-LS6H-KQ	KQ-QS9H-KQ
细菌 Bacteria/ （10⁶ CFU·g^-1）	2020	6.03 αbc	6.93 αb	5.20 αc	9.70 αa	5.83 βbc	7.07 βb	5.83 βbc	10.53 αa	10.77 αa	10.93 αa
	2021	5.07 βe	6.20 αd	2.83 βf	6.37 βd	10.00 αb	8.93 αc	11.30 αa	6.43 γd	6.37 βd	6.07 βd
	2022	4.20 γcd	3.70 βd	3.77 αβd	6.70 βb	5.00 βc	6.50 βb	6.30 βb	8.10 βa	6.40 βb	6.53 βb
真菌 Fungi/ （10² CFU·g^-1）	2020	3.67 γb	2.67 βb	1.67 γb	11.67 αa	4.33 βb	2.00 γb	2.00 γb	10.00 βa	11.67 αβa	13.67 βa
	2021	52.33 αa	22.00 αbc	19.00 βcd	14.00 αdef	17.67 αcde	5.00 βg	13.00 βef	10.00 βf	16.00 αdef	25.00 αb
	2022	13.00 βde	19.33 αc	41.00 αa	15.00 αcd	13.67 αde	16.33 αcd	36.33 αb	16.33 αcd	9.00 βef	6.00 γf
放线菌 Actinomycetes/ （10⁵ CFU·g^-1）	2020	7.33 αb	6.93 βb	5.23 βc	10.73 αβa	7.57 βb	7.40 βb	5.37 γc	10.20 αa	10.77 αa	10.67 αa
	2021	7.90 αc	9.60 αab	6.77 αc	9.23 βb	9.80 αab	10.83 αa	10.70 αa	11.03 αa	10.77 αa	9.80 αab
	2022	5.90 βbc	5.30 γbc	5.43 βbc	12.40 αa	6.33 γbc	5.20 γc	6.97 βb	11.90 αa	11.87 αa	11.40 αa
总计 Total/ （10⁶ CFU·g^-1）	2020	6.77 αbcd	7.63 αbc	5.72 αd	10.77 αa	6.59 βbcd	7.81 βb	6.37 βcd	11.55 αa	11.84 αa	12.00 αa
	2021	5.86 βe	7.16 αd	3.51 βf	7.29 βd	10.98 αb	10.02 αc	12.37 αa	7.54 γd	7.45 βd	7.05 βd
	2022	4.79 γcd	4.23 βd	4.31 αβd	7.94 βb	5.63 βc	7.02 βb	7.00 βb	9.29 βa	7.59 βb	7.67 βb

指标 Index	年份 Year	处理Treatment
指标 Index	年份 Year	HZ88-HZ88-HZ88	LS6H-LS6H-LS6H	QS9H-QS9H-QS9H	KQ- KQ-KQ	HZ88-KQ-HZ88	LS6H-KQ-LS6H	QS9H-KQ-QS9H	KQ-HZ88-KQ	KQ-LS6H-KQ	KQ-QS9H-KQ
细菌 Bacteria/ （10⁶ CFU·g^-1）	2020	6.03 αbc	6.93 αb	5.20 αc	9.70 αa	5.83 βbc	7.07 βb	5.83 βbc	10.53 αa	10.77 αa	10.93 αa
	2021	5.07 βe	6.20 αd	2.83 βf	6.37 βd	10.00 αb	8.93 αc	11.30 αa	6.43 γd	6.37 βd	6.07 βd
	2022	4.20 γcd	3.70 βd	3.77 αβd	6.70 βb	5.00 βc	6.50 βb	6.30 βb	8.10 βa	6.40 βb	6.53 βb
真菌 Fungi/ （10² CFU·g^-1）	2020	3.67 γb	2.67 βb	1.67 γb	11.67 αa	4.33 βb	2.00 γb	2.00 γb	10.00 βa	11.67 αβa	13.67 βa
	2021	52.33 αa	22.00 αbc	19.00 βcd	14.00 αdef	17.67 αcde	5.00 βg	13.00 βef	10.00 βf	16.00 αdef	25.00 αb
	2022	13.00 βde	19.33 αc	41.00 αa	15.00 αcd	13.67 αde	16.33 αcd	36.33 αb	16.33 αcd	9.00 βef	6.00 γf
放线菌 Actinomycetes/ （10⁵ CFU·g^-1）	2020	7.33 αb	6.93 βb	5.23 βc	10.73 αβa	7.57 βb	7.40 βb	5.37 γc	10.20 αa	10.77 αa	10.67 αa
	2021	7.90 αc	9.60 αab	6.77 αc	9.23 βb	9.80 αab	10.83 αa	10.70 αa	11.03 αa	10.77 αa	9.80 αab
	2022	5.90 βbc	5.30 γbc	5.43 βbc	12.40 αa	6.33 γbc	5.20 γc	6.97 βb	11.90 αa	11.87 αa	11.40 αa
总计 Total/ （10⁶ CFU·g^-1）	2020	6.77 αbcd	7.63 αbc	5.72 αd	10.77 αa	6.59 βbcd	7.81 βb	6.37 βcd	11.55 αa	11.84 αa	12.00 αa
	2021	5.86 βe	7.16 αd	3.51 βf	7.29 βd	10.98 αb	10.02 αc	12.37 αa	7.54 γd	7.45 βd	7.05 βd
	2022	4.79 γcd	4.23 βd	4.31 αβd	7.94 βb	5.63 βc	7.02 βb	7.00 βb	9.29 βa	7.59 βb	7.67 βb

指标 Index	年份 Year	处理Treatment
指标 Index	年份 Year	HZ88-HZ88-HZ88	LS6H-LS6H-LS6H	QS9H-QS9H-QS9H	HZ88-KQ-HZ88	LS6H-KQ-LS6H		QS9H-KQ-QS9H		KQ-HZ88-KQ	KQ-LS6H-KQ		KQ- QS9H-KQ
单株产量 Yield per plant/kg	2020	0.39 βa	0.42 βa	0.44 βa	0.39 a	0.43 a		0.41 a		—		—		—
	2021	0.38 βcd	0.42 βc	0.33 γd	—		—		—	0.67 b	0.76 a		0.63 b
	2022	0.76 αc	0.87 αab	0.75 αc	0.77 c	0.84 b		0.92 a		—		—		—
淀粉含量 Starch content/%	2020	14.49 αβa	10.88 βc	13.13 αab	14.35 a	10.92 c		12.67 b		—		—		—
	2021	15.41 αa	11.91 βc	11.28 αc	—		—		—	14.10 ab	12.77 bc		12.89 bc
	2022	13.29 βb	15.53 αa	12.03 αb	15.16 a	12.96 b		13.18 b		—		—		—
还原糖含量 Reducing sugar content/%	2020	0.12 γc	0.18 γab	0.15 βbc	0.12 c	0.19 a		0.13 c		—		—		—
	2021	0.31 αb	0.38 αa	0.28 αb	—		—		—	0.19 c	0.31 b		0.22 c
	2022	0.27 βa	0.26 βa	0.17 βb	0.13 c	0.13 c		0.18 b		—		—		—