Analysis of Genetic Diversity and Population Structure of Flax Local Varieties in Gansu

doi:10.13304/j.nykjdb.2024.0222

Abstract

Abstract:

Flax is a major oilseed crop in arid regions of China， and Gansu is one of the main production areas of flax in China. Studying the genetic diversity and population structure of flax local varieties in Gansu province is of great significance for uncovering rich and superior allelic variations and improving sesame breeding efficiency. Using SSR molecular marker， the genetic diversity and population structure of 94 flax local varieties from 13 counties （cities and districts） in Gansu province were analyzed. The results showed that 46 alleles were got by 21 SSR primers with 2~3 alleles per primer； the polymorphism information contents were 0.158~0.522 with an average of 0.343， the observed heterozygosity （H_o） was 0.060~0.957 with an average of 0.448， the expected heterozygosity （H_e） was 0.126~0.569 with an average of 0.408， and the Shannon diversity index were 0.267~0.860 with an average of 0.613. With a genetic distance threshold of 0.028， the 94 sesame resources were grouped into 4 groups. It showed that the genetic differences between sesame local varieties in Gansu province were small， the genetic distances were close， and the genetic background was narrow.

Key words: flax, local variety, genetic diversity, population structure

摘要：

胡麻是我国干旱地区的主要油料作物，甘肃是我国胡麻主要产区之一。研究甘肃胡麻地方品种的遗传多样性和群体结构，对于挖掘丰富优异等位变异、提高胡麻育种效率具有重要意义。利用SSR分子标记对甘肃省13个县（市、区）的94份胡麻地方品种资源进行遗传多样性和群体结构分析。结果表明，21对SSR引物共扩增出46个等位变异，每对引物有2～3个等位变异；多态信息含量为0.158~0.522，平均为0.343；观测杂合度为0.060~0.957，平均0.448；期望杂合度为0.126~0.569，平均0.408；香农多样性指数为0.267~0.860，平均0.613；以遗传距离0.028为阈值，94份胡麻地方品种被聚为4个类群。由此表明，甘肃胡麻地方品种间遗传差异较小、遗传距离相近、遗传背景狭窄。

关键词: 胡麻, 地方品种, 遗传多样性, 群体结构

CLC Number:

S563.2

Jie LIU, Bin WANG, Jingjing HOU, Bing WU, Li ZHAO. Analysis of Genetic Diversity and Population Structure of Flax Local Varieties in Gansu[J]. Journal of Agricultural Science and Technology, 2025, 27(9): 69-78.

刘杰, 王斌, 侯静静, 吴兵, 赵利. 甘肃胡麻地方品种遗传多样性和群体结构分析[J]. 中国农业科技导报, 2025, 27(9): 69-78.

Figures/Tables 6

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引物名称 Primer name	引物序列 Primer sequence （5’-3’）
m10	GGGATGCTGATGAGGAAG	GGAGGAGACAGAGGTGGA
LGM-61	GCCTGCAGTTTAGTCGTTGG	CGGAAAGAACAATTCCAGCTC
LU28	TCCCAGCGAGTTTGGTGAG	TGGAGGAACTAATTGTGGCAAG
LU3	GCTCGTGATCTCCTTCATCC	AAAACCACGTCCAGATGCTC
LU7	CATCCAACAAAGGGTGGTG	GGAACAAAGGGTAGCCATGA
LU8	TCCCGTAATATTCTATGTTCTTCC	TGAGTTGGACCTTACAAGACTCA
LU15	TGGACGACGATGAAGATGAA	CCGCCGGGTACACTACTACT
LU18	AGAGGCGGAGGGCATTAC	TTGGAGAGTTGGAATCGAGA
101	AGGAAGAGGTAGCCCAGTCC	AGACTCACGGTGAAGGCAAC
35L	ACGTCGAGGAGAAGGGAGAT	AATGTCCGTCTCCCACAAAC
SR3572	TCTCGTAGCTAGGGAGATGG	AAAGCCGTCGTACTCACCAC
scaffold993	CAGATCGATGAACTCCTCCTCA	GCTGCTTTTGTTGTTGTTGGAG
scaffold231	TTGAGGTGCAGCTTAACAGAGC	AATGGGTTTCAGCAGCTTCTTC
sfd584	TCATTTCTCCAACCAGCTGAAA	CAAATCTCCGGACCAGACTCTT
scaffold25	TGGAGCTTCTTCATCTGCTTTG	GGATTCAACCGACTTGGGATAA
Lu291	GGAAATTCCAAGTTCCCAGT	AGTTTCGCTATTCCGTCTGC
Lu672	GGATTTGACGCTGGGTGTAG	GGATTTGACGCTGGGTGTAG
Lu836	AAGATAGGGGGACGCAGAAT	AAGATAGGGGGACGCAGAAT
Lu2553	TGGGAGAAATTATCCACTACGC	TGGGAGAAATTATCCACTACGC
Lu2565	AACCAAGAGGCTTCATACGG	AACCAAGAGGCTTCATACGG
Lu2996	GGCAGACTCTCGCTGGTTAG	GGCAGACTCTCGCTGGTTAG

引物名称 Primer name	引物序列 Primer sequence （5’-3’）
m10	GGGATGCTGATGAGGAAG	GGAGGAGACAGAGGTGGA
LGM-61	GCCTGCAGTTTAGTCGTTGG	CGGAAAGAACAATTCCAGCTC
LU28	TCCCAGCGAGTTTGGTGAG	TGGAGGAACTAATTGTGGCAAG
LU3	GCTCGTGATCTCCTTCATCC	AAAACCACGTCCAGATGCTC
LU7	CATCCAACAAAGGGTGGTG	GGAACAAAGGGTAGCCATGA
LU8	TCCCGTAATATTCTATGTTCTTCC	TGAGTTGGACCTTACAAGACTCA
LU15	TGGACGACGATGAAGATGAA	CCGCCGGGTACACTACTACT
LU18	AGAGGCGGAGGGCATTAC	TTGGAGAGTTGGAATCGAGA
101	AGGAAGAGGTAGCCCAGTCC	AGACTCACGGTGAAGGCAAC
35L	ACGTCGAGGAGAAGGGAGAT	AATGTCCGTCTCCCACAAAC
SR3572	TCTCGTAGCTAGGGAGATGG	AAAGCCGTCGTACTCACCAC
scaffold993	CAGATCGATGAACTCCTCCTCA	GCTGCTTTTGTTGTTGTTGGAG
scaffold231	TTGAGGTGCAGCTTAACAGAGC	AATGGGTTTCAGCAGCTTCTTC
sfd584	TCATTTCTCCAACCAGCTGAAA	CAAATCTCCGGACCAGACTCTT
scaffold25	TGGAGCTTCTTCATCTGCTTTG	GGATTCAACCGACTTGGGATAA
Lu291	GGAAATTCCAAGTTCCCAGT	AGTTTCGCTATTCCGTCTGC
Lu672	GGATTTGACGCTGGGTGTAG	GGATTTGACGCTGGGTGTAG
Lu836	AAGATAGGGGGACGCAGAAT	AAGATAGGGGGACGCAGAAT
Lu2553	TGGGAGAAATTATCCACTACGC	TGGGAGAAATTATCCACTACGC
Lu2565	AACCAAGAGGCTTCATACGG	AACCAAGAGGCTTCATACGG
Lu2996	GGCAGACTCTCGCTGGTTAG	GGCAGACTCTCGCTGGTTAG

引物Primer	N_a	N_e	PIC	H_o	H_e	I	F_st	N_m
m10	3	1.704	0.369	0.457	0.415	0.730	0.030	8.093
LGM-61	2	1.353	0.227	0.309	0.262	0.430	0.014	17.634
LU28	2	1.837	0.352	0.702	0.458	0.614	0.005	52.071
LU3	2	1.826	0.350	0.692	0.455	0.627	0.011	23.148
LU7	2	1.972	0.439	0.117	0.495	0.644	0.051	4.626
LU8	3	2.305	0.522	0.400	0.569	0.645	0.010	25.916
LU15	3	1.987	0.441	0.628	0.499	0.648	0.012	20.478
LU18	2	1.732	0.333	0.606	0.425	0.654	0.046	5.150
101	2	1.929	0.366	0.809	0.484	0.675	0.008	30.151
35L	2	1.937	0.367	0.819	0.486	0.677	0.006	41.212
SR3572	2	1.996	0.375	0.511	0.502	0.692	0.007	34.529
scaffold993	2	1.665	0.416	0.552	0.402	0.589	0.016	15.365
scaffold231	2	1.996	0.375	0.958	0.502	0.692	0.002	151.690
sfd584	2	1.209	0.158	0.192	0.174	0.316	0.060	3.943
scaffold25	3	2.067	0.444	0.404	0.519	0.852	0.008	33.264
Lu291	2	1.162	0.148	0.060	0.140	0.267	0.045	5.351
Lu672	2	1.398	0.261	0.108	0.286	0.459	0.009	29.635
Lu836	2	1.252	0.187	0.223	0.203	0.396	0.010	25.828
Lu2553	2	1.858	0.355	0.170	0.464	0.855	0.007	34.298
Lu2565	2	1.824	0.408	0.200	0.454	0.860	0.032	7.609
Lu2996	2	1.586	0.301	0.489	0.372	0.556	0.030	8.038

引物Primer	N_a	N_e	PIC	H_o	H_e	I	F_st	N_m
m10	3	1.704	0.369	0.457	0.415	0.730	0.030	8.093
LGM-61	2	1.353	0.227	0.309	0.262	0.430	0.014	17.634
LU28	2	1.837	0.352	0.702	0.458	0.614	0.005	52.071
LU3	2	1.826	0.350	0.692	0.455	0.627	0.011	23.148
LU7	2	1.972	0.439	0.117	0.495	0.644	0.051	4.626
LU8	3	2.305	0.522	0.400	0.569	0.645	0.010	25.916
LU15	3	1.987	0.441	0.628	0.499	0.648	0.012	20.478
LU18	2	1.732	0.333	0.606	0.425	0.654	0.046	5.150
101	2	1.929	0.366	0.809	0.484	0.675	0.008	30.151
35L	2	1.937	0.367	0.819	0.486	0.677	0.006	41.212
SR3572	2	1.996	0.375	0.511	0.502	0.692	0.007	34.529
scaffold993	2	1.665	0.416	0.552	0.402	0.589	0.016	15.365
scaffold231	2	1.996	0.375	0.958	0.502	0.692	0.002	151.690
sfd584	2	1.209	0.158	0.192	0.174	0.316	0.060	3.943
scaffold25	3	2.067	0.444	0.404	0.519	0.852	0.008	33.264
Lu291	2	1.162	0.148	0.060	0.140	0.267	0.045	5.351
Lu672	2	1.398	0.261	0.108	0.286	0.459	0.009	29.635
Lu836	2	1.252	0.187	0.223	0.203	0.396	0.010	25.828
Lu2553	2	1.858	0.355	0.170	0.464	0.855	0.007	34.298
Lu2565	2	1.824	0.408	0.200	0.454	0.860	0.032	7.609
Lu2996	2	1.586	0.301	0.489	0.372	0.556	0.030	8.038

来源地Source	陇东LD	陇南LN	中部ZB
陇南LN	0.977
中部ZB	0.984	0.991
河西HE	0.983	0.985	0.986