Screening of Combination of Flumetralin and Fungicide to Control Cotton Fusarium wilt

doi:10.13304/j.nykjdb.2022.0884

Abstract

Abstract:

In order to screen the combination of flutenide and fungicide for effective controling of cotton Fusarium wilt and determine the optimal ratio of agents， Fusarium oxysporum， the pathogen causing cotton wilt， was taken as the research object， the growth rate method was used to measure the indoor toxicity of fungicide， Wadley’s synergistic ratio method was used to evaluate the synergistic effect of the compounds， and the indoor pot experiment was conducted to verify the effect of the compounds on cotton Fusarium wilt. The results showed that the effective inhibitory medium concentration （EC₅₀） of flufluenil， pyrimethil， Bacillus subtilis and flutamide were 0.219， 0.725， 6.013 and 55.971 mg·L^-1， respectively. Compound of flufenamide and fluoxonitrile showed synergistic effect when the compound ratio was 10∶1. The compound of flutenediamine and Bacillus subtilis showed synergic effects when the compound ratio was 10∶1， 3∶1， 1∶2 and 1∶3， and the synergic effect was the highest when the ratio was 1∶2. While the compound of flutamide and pyrimethanil showed no synergistic effect. Pot experiments showed that the disease index was the lowest when flutenediamine was mixed with Bacillus subtilis with the ratio of 1∶2， and the control effect was 77.11%， which was significantly better than other combinations. In conclusion， the combination between flutamide and Bacillus subtilis with the ratio of 1∶2 could be used for preventing cotton Fusarium wilt. Above results provided technical guidance for prevention and control of cotton Fusarium wilt.

Key words: cotton, Fusarium wilt, screening, flumetralin, fungicide, synergistic coefficient

摘要：

为筛选有效防治棉花枯萎病的氟节胺与杀菌剂组合，并明确最佳药剂配比，以棉花枯萎病致病菌尖孢镰刀菌（Fusarium oxysporum）为研究对象，采用菌丝生长速率法测定杀菌剂的毒力，采用Wadley的增效比率法评价复配剂的增效作用，通过室内盆栽试验验证其对棉花枯萎病的防治效果。结果显示，咯菌腈、嘧菌酯、枯草芽孢杆菌和氟节胺的有效抑制中浓度（EC₅₀）分别为0.219、0.725、6.013、55.971 mg·L^-1。氟节胺与咯菌腈在复配比为10∶1时具有增效作用；氟节胺与枯草芽孢杆菌在复配比为10∶1、3∶1、1∶2和1∶3时均具有增效作用，其中复配比为1∶2时增幅最大；氟节胺与嘧菌酯复配无增效作用。室内盆栽试验结果表明，氟节胺与枯草芽孢杆菌按1∶2复配时棉花的病情指数最低，防治效果达77.11%，显著优于其他复配组合。综上所述，当棉花发生枯萎病时可使用氟节胺与枯草芽孢杆菌按1∶2复配进行防治。以上研究结果为防治棉花枯萎病提供了技术指导。

关键词: 棉花, 枯萎病, 药剂筛选, 氟节胺, 杀菌剂, 增效系数

CLC Number:

S432.1

Deyou ZHENG, Dongyun ZUO, Qiaolian WANG, Limin LYU, Hailiang CHENG, Aixing GU, Guoli SONG. Screening of Combination of Flumetralin and Fungicide to Control Cotton Fusarium wilt[J]. Journal of Agricultural Science and Technology, 2024, 26(1): 119-124.

郑德有, 左东云, 王巧莲, 吕丽敏, 程海亮, 顾爱星, 宋国立. 氟节胺与杀菌剂复配防治棉花枯萎病的增效药剂筛选[J]. 中国农业科技导报, 2024, 26(1): 119-124.

Figures/Tables 5

References 24

1	SHAAPULATOV U M, BURIEV Z T, ULLOA M. Characterization of small RNAs and their targets from Fusarium oxysporum infectedand noninfected cotton root tissues [J]. Plant Mol. Biol. Rep., 2016, 34(3):698-706.
2	沈其益.棉花病害:基础研究与防治[M].北京:科学出版社,1992:128-151.
3	陈丽华,何鹏飞,袁德超.一种防治棉花黄萎病的生物复合种衣剂的研制[J].棉花学报,2018,30(3):282-291.
	CHEN L H, HE P F, YUAN D C, et al.. Development of a biological complex seed-coating agent to control Verticillium wilt [J]. Cotton Sci., 2018, 30(3):282-291.
4	袁传卫,姜兴印,殷万元,等.吡唑醚菌酯对土壤微生物呼吸作用及土壤酶活性的影响[J].农业环境科学学报,2015,34(5):897-903.
	YUAN C W, JIANG X Y, YIN W Y, et al.. Effects of pyraclostrobin on soil microbial respiration and enzymatic activities [J]. J. Agro-Environ. Sci., 2015, 34(5):897-903.
5	雷斌,周小云,刘梦丽,等.8.2%萎锈灵·咯菌腈包衣对棉花幼苗耐寒性的生理特征的影响[J].农药,2022,61(3):230-234.
	LEI B, ZHOU X Y, LIU M L, et al.. Effects of 8.2%carboxin-fludioxonil from seed coating formulation onphysiological characteristics of cold tolerance of cotton [J]. Agrochemicals, 2022, 61(3):230-234.
6	王继雯,岳丹丹,李冠杰,等.枯草芽孢杆菌菌剂对冬小麦生理生化指标的影响[J].河南科学,2020,38(3):397-403.
	WANG J W, YUE D D, LI G J, et al.. Effects of Bacillus subtilis on physiological and biochemical indexes of winter wheat [J]. Henan Sci., 2020, 38(3):397-403.
7	杨兴洪,陈翠容,施培.植物生长调节剂在棉花上的研究应用概况及展望[J].山东农业大学学报,1996,27(2):236-240.
	YANG X H, CHEN C R, SHI P. Advances and prospects for applications and studies of PGR on cotton [J]. J. Shandong Agric. Univ., 1996, 27(2):236-240.
8	李田甜,陈国栋,万素梅,等.叶面喷施不同配方植物生长调节剂对棉花苗期根系生长的影响[J].山东农业科学,2022,54(2):46-50.
	LI T T, CHEN G D, WAN S M, et al.. Effects of foliar application of plant growth regulators with different formulaon root growth of cotton at seedling stage [J]. Shandong Agric. Sci., 2022, 54(2):46-50.
9	PENNUCCI A, JACKSON N. Distinguishing phytotoxictty and pathogenicity by fungi in growth retardant treated turfgrass [J]. Phytopathology, 1986, 76(6):657-657.
10	霍成君,韩建国,毛培胜,等.矮壮素和多效唑对草地早熟禾草坪质量的影响[J].草地学报,2000,8(2):137-143.
	HUO C J, HAN J G, MAO P S, et al.. Effects of plant growth regulator on the turf quality of kentucky bluegrass [J]. Acta Agrestia Sin., 2000,8(2):137-143.
11	朱荷琴,冯自力,师勇强,等.利用植物疫苗及生长调节剂缩节胺控制棉花黄萎病[J].中国棉花,2010,37(8):10-12.
12	蒋家珍,姜世聚,邱立红,等.油菜素内酯与丙环唑互作对棉苗病害的影响及机理研究[J].湖南农业科学,2004(5):49-51.
	JIANG J Z, JIANG S J, QIU L H, et al.. Effects of brassinolide and propiconazol combinationon cotton seeding diseases [J]. Hunan Agric. Sci., 2004(5):49-51.
13	中国标准化研究院. 微生物源抗生素类次生代谢产物抗真菌活性测定菌丝生长速率法 [S].北京: 中国标准出版社,2020.
14	李海薇,侯仙,王梦瑶,等.海岛棉枯萎病病原菌分离及室内药剂筛选[J].中国农学通报,2019,35(9):68-74.
	LI H W, HOU X, WANG M Y, et al.. Pathogen isolation of Fusarium wilt of island cotton and indoor fungicides screening [J]. Chin. Agric. Sci. Bull., 2019, 35(9):68-74.
15	檀根甲,祝建平.杀菌剂生物测定计算方法及应用[J].安徽农学通报,1998(1):28-29.
16	WADLEY F M. Experimental Statistics in Entomology [M]. Washington, D. C.: Graduate School Press, 1967: 1-211.
17	姜佳,陈金鹏,魏江桥,等.咯菌腈与戊唑醇复配对小麦赤霉病菌的增效作用研究[J].中国农学通报,2022,38(6):116-120.
	JIANG J, CHEN J P, WEI J Q, et al.. Synergistic effect of fludioxonil and tebuconazole against Fusarium graminearum [J]. Chin. Agric. Sci. Bull., 2022, 38(6):116-120.
18	朱荷琴,冯自力,李志芳,等.蛭石沙土无底纸钵定量蘸菌液法鉴定棉花品种(系)的抗黄萎病性[J].中国棉花,2010,37(12):15-17.
19	周京龙,冯自力,魏锋,等.棉花内生细菌YUPP-10及其分泌蛋白CGTase对棉花枯萎病的防治作用及机理[J].中国农业科学,2021,54(17):3691-3701.
	ZHOU J L, FENG Z L, WEI F, et al.. Biocontrol effect and mechanism of cotton endophytic bacterium YUPP-10 and its secretory protein CGTase against Fusarium wilt in cotton [J]. Sci. Agric. Sin., 2021, 54(17):3691-3701.
20	中华人民共和国农业部. 棉花抗病虫性评价技术规范第4部分:枯萎病: [S].北京:中国标准出版社,2009.
21	李海薇,韩万里,王梦瑶,等.棉花枯萎病拮抗短小芽胞杆菌筛选鉴定及生防研究[J].中国生物防治学报,2018,34(3):440-448.
	LI H W, HAN W L, WANG M Y, et al.. Screening and identification of antagonistic bacteria Bacillus pumilus against cotton Fusarium wilt and biocontrol research [J]. J. Chin. Biol. Control, 2018, 34(3):440-448.
22	李玉洋,辛寒晓,范学明,等.尖孢镰刀菌拮抗菌SN06的分离、鉴定及其对棉花枯萎病的防治效果[J].山东农业科学,2017,49(3):117-122.
	LI Y Y, XIN H X, FAN X M, et al.. Isolation and identification of Fusarium oxysporum antagonistic strain SN06 and its control effect on cotton Fusarium wilt [J]. Shandong Agric. Sci., 2017, 49(3):117-122.
23	么文超,蒋勤军,韩烈保.植物生长调节剂与杀菌剂互作对早熟禾夏季斑的防治[J].草地学报,2009,17(3):371-376.
	YAO W C, JIANG Q J, HAN L B, et al.. Synergistic action of plant growth regulators and fungicides on control efficiency of summer patchin kentucky bluegrass [J]. Acta Agrestia Sin., 2009, 17(3):371-376.
24	王成菊,李常平,郑明奇,等.植物生长调节剂与杀菌剂互作对棉花立枯病的影响[J].农药,2003,42(12):36-38.
	WANG C J, LI C P, ZHENG M Q, et al.. The combination effect of plant growth regulators and fungicides to damping-off [J]. Agrochemicals, 2003,42(12):36-38.

杀菌剂 Fungicide	毒力回归方程 Virulence regression equation	EC₅₀/（mg·L^-1）	决定系数R²
咯菌腈Fludioxonil	y=-0.527+0.800x	0.219 d	0.976 ^*
嘧菌酯Azoxystrobin	y=0.101+0.723x	0.725 c	0.903 ^*
枯草芽孢杆菌Bacillus subtilis	y=-0.354+0.454x	6.013 b	0.975 ^*
氟节胺Flumetralin	y=-2.368+1.355x	55.971 a	0.934 ^*

杀菌剂 Fungicide	毒力回归方程 Virulence regression equation	EC₅₀/（mg·L^-1）	决定系数R²
咯菌腈Fludioxonil	y=-0.527+0.800x	0.219 d	0.976 ^*
嘧菌酯Azoxystrobin	y=0.101+0.723x	0.725 c	0.903 ^*
枯草芽孢杆菌Bacillus subtilis	y=-0.354+0.454x	6.013 b	0.975 ^*
氟节胺Flumetralin	y=-2.368+1.355x	55.971 a	0.934 ^*

氟节胺∶咯菌腈 Flumetralin∶fludioxonil	毒力回归方程 Virulence regression equation	决定系数R²	EC₅₀/（mg·L^-1）	增效系数 Synergistic coefficient
10∶1	y=-0.077+1.091x	0.941^*	1.177	1.970
8∶1	y=-0.334+1.547x	0.981^*	1.644	1.163
5∶1	y=-0.202+1.252x	0.952^*	1.450	0.889
3∶1	y=-0.005+1.070x	0.958^*	1.010	0.857
2∶1	y=-0.072+1.107x	0.989^*	1.161	0.561
1∶1	y=-0.094+0.907x	0.927^*	0.788	0.554
1∶2	y=-0.074+0.942x	0.992^*	1.199	0.273
1∶3	y=-0.115+0.841x	0.933^*	1.372	0.213
1∶5	y=-0.078+0.877x	0.936^*	0.815	0.322
1∶8	y=-0.127+0.946x	0.995^*	1.363	0.181
1∶10	y=-0.221+0.905x	0.931^*	1.755	0.137

氟节胺∶咯菌腈 Flumetralin∶fludioxonil	毒力回归方程 Virulence regression equation	决定系数R²	EC₅₀/（mg·L^-1）	增效系数 Synergistic coefficient
10∶1	y=-0.077+1.091x	0.941^*	1.177	1.970
8∶1	y=-0.334+1.547x	0.981^*	1.644	1.163
5∶1	y=-0.202+1.252x	0.952^*	1.450	0.889
3∶1	y=-0.005+1.070x	0.958^*	1.010	0.857
2∶1	y=-0.072+1.107x	0.989^*	1.161	0.561
1∶1	y=-0.094+0.907x	0.927^*	0.788	0.554
1∶2	y=-0.074+0.942x	0.992^*	1.199	0.273
1∶3	y=-0.115+0.841x	0.933^*	1.372	0.213
1∶5	y=-0.078+0.877x	0.936^*	0.815	0.322
1∶8	y=-0.127+0.946x	0.995^*	1.363	0.181
1∶10	y=-0.221+0.905x	0.931^*	1.755	0.137

氟节胺∶枯草芽孢杆菌 Flumetralin∶Bacillus subtilis	毒力回归方程 Virulence regression equation	决定系数R²	EC₅₀/（mg·L^-1）	增效系数 Synergistic coefficient
10∶1	y=-1.438+1.164x	0.927^*	17.211	1.944
8∶1	y=-1.670+1.236x	0.907^*	22.460	1.367
5∶1	y=-1.091+0.619x	0.964^*	57.748	0.434
3∶1	y=-0.672+0.603x	0.944^*	10.404	1.887
2∶1	y=-1.785+1.425x	0.892^*	17.876	0.903
1∶1	y=-1.683+1.548x	0.954^*	12.224	0.974
1∶2	y=-0.468+0.877x	0.928^*	3.422	2.755
1∶3	y=-0.873+1.290x	0.921^*	4.751	1.798
1∶5	y=-1.121+0.762x	0.899^*	29.660	0.263
1∶8	y=-1.803+1.214x	0.917^*	30.549	0.242
1∶10	y=-1.478+1.428x	0.942^*	10.839	0.668