Identification and Expression Analysis of Calmodulin-binding Transcription Activator （CAMTA） Family Genes in Tea Plants

doi:10.13304/j.nykjdb.2023.0591

Abstract

Abstract:

In order to understand calmodulin-binding transcription activator （CAMTA） family members in tea plants and explore their expression characteristics， the CsCAMTAs were identified in the whole genome of tea plants， and their physicochemical properties， gene structure， domain， conserved motif order， phylogenetic relationship and cis-acting elements were analyzed by bioinformatics method， and the expression of CsCAMTAs in different tissues， under different hormone treatments and bacterial stress were analyzed by qRT-PCR. The results showed that a total of 10 CsCAMTAs members were identified with 10 motifs， which distributed in 6 chromosomes and all localized in the nucleus. The sizes of different CsCAMTAs gene were different with the number of amino acids from 124 to 1 419， the isoelectric points of the coding protein were from 4.976 to 9.820， and only 1 was weak hydrophobic protein， the others were hydrophilic proteins. Phylogenetic analysis showed that the CsCAMTAs were clustered into 4 groups， with 4 homologous gene pairs， and the homologous gene pairs had high similarity in gene structure， conserved domain， number of conserved motifs and relative position. The promoter region of CsCAMTAs contained 34 cis-acting elements associated with light response， hormone induction， and biotic stress. The expression analysis results showed that the expressions of CsCAMTAs in different tissues were significantly different. CsCAMTA1， 3，4， 7， 8， 10 had higher expression level in mature leaves. Except for CsCAMTA5， which was not induced by methyl jasmonate， the others were induced by methyl jasmonate， abscisic acid， salicylic acid and tea wheel spot pathogen， which indicated that CsCAMTAs not only participated in the regulation of tea plants growth and development， but also participated in the response of tea plants to bacteria and hormones.

Key words: tea plants, calmodulin-binding transcriptional activator （CAMTA）, bioinformatics analysis, expression analysis

摘要：

为了解茶树钙调蛋白结合转录激活因子（calmodulin-binding transcription activator，CAMTA）家族成员的功能，探究其表达特性，利用生物信息学方法在茶树全基因组中鉴定CsCAMTAs基因，并对其理化性质、基因结构、结构域、保守基序、系统进化、顺式作用元件进行分析，利用qRT-PCR技术分析CsCAMTAs在不同组织、不同激素处理和病菌胁迫下的表达情况。结果表明，在茶树全基因组中共鉴定出10个CsCAMTAs成员，共包含10个motifs，分布在6条染色体，均定位于细胞核。不同CsCAMTAs基因差异较大，氨基酸数目为124~1 419个，编码蛋白等电点为4.976~9.820，除1个为弱疏水蛋白外，其他均为亲水蛋白。系统进化分析将茶树10个CsCAMTAs基因划分为4个类群，有4个同源基因对，同源基因对在基因结构、保守结构域、保守基序数量和相对位置等方面具有较高相似性。CsCAMTAs启动子区含有34个与光响应、激素诱导以及生物胁迫相关的顺式作用元件。表达分析结果显示，CsCAMTAs在不同组织的表达有明显差异，其中CsCAMTA1、3、4、7、8、10在老叶中的表达水平较高；除CsCAMTA5不受茉莉酸甲酯诱导外，其他CsCAMTAs基因均受茉莉酸甲酯、脱落酸、水杨酸及茶轮斑病菌诱导表达，由此说明CsCAMTAs不仅调控茶树生长发育，还参与茶树对病菌和激素的应答。

关键词: 茶树, 钙调蛋白结合转录激活因子（CAMTA）, 生物信息学分析, 表达分析

CLC Number:

S571.1

Jinxian LIU, Lijuan WANG, Jie LIU, Xianyu FU, Guangheng WU. Identification and Expression Analysis of Calmodulin-binding Transcription Activator （CAMTA） Family Genes in Tea Plants[J]. Journal of Agricultural Science and Technology, 2025, 27(3): 71-82.

刘金仙, 王丽娟, 刘杰, 傅仙玉, 武广珩. 茶树钙调蛋白结合转录激活因子（CAMTA）家族基因鉴定与表达分析[J]. 中国农业科技导报, 2025, 27(3): 71-82.

Figures/Tables 11

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基因 Gene	引物名称 Primer name	引物序列 Primer sequence （5’-3’）
CsCAMAT10	RT-CsCAMAT10-F	GCCATAGGAGCCTTAGCATA
CsCAMAT10	RT-CsCAMAT10-R	CCAGGGCAAACTCAACAACT
CsCAMAT9	RT-CsCAMAT9-F	TGATCCTTTGGTGCAGAACG
CsCAMAT9	RT-CsCAMAT9-R	CCAATCCAGCAACCATGTGA
CsCAMAT8	RT-CsCAMAT8-F	AGCATAATCTGGTCCGTTGG
CsCAMAT8	RT-CsCAMAT8-R	CGTTCTTCTGTTTGCTTTCG
CsCAMAT7	RT-CsCAMAT7-F	GCCATAACTGGAGGAAGAAG
CsCAMAT7	RT-CsCAMAT7-R	ATAGCTTCGCCTCTGGAAAT
CsCAMAT6	RT-CsCAMAT6-F	GCTGCTCGCATTCATCAAGT
CsCAMAT6	RT-CsCAMAT6-R	GAATCCACGCAAACCACTCC
CsCAMAT5	RT-CsCAMAT5-F	GTCCGCAGATGTTACTGGCTAC
CsCAMAT5	RT-CsCAMAT5-R	GCACTCAACTTGCTGGGATC
CsCAMAT4	RT-CsCAMAT4-F	AGGATGGCAAGACCGTCAAA
CsCAMAT4	RT-CsCAMAT4-R	GTTCAAGGGCAACCGCACCA
CsCAMAT3	RT-CsCAMAT3-F	GTTCTCCCGTCACTCCTGTC
CsCAMAT3	RT-CsCAMAT3-R	CCCTTCACGCTGATGATGTA
CsCAMAT2	RT-CsCAMAT2-F	TATGTTGTCTGGCTCCTCCC
CsCAMAT2	RT-CsCAMAT2-R	CGGCTGATTACATCCTCCTC
CsCAMAT1	RT-CsCAMAT1-F	GGAAATTGTGAAATGGGCAGTC
CsCAMAT1	RT-CsCAMAT1-R	TCCTCATCGTCATCGGCATC
CsGAPDH	RT-CsGAPDH-F	TTGGCATCGTTGAGGGTCT
CsGAPDH	RT-CsGAPDH-R	CAGTGGGAACACGGAAAGC

基因 Gene	引物名称 Primer name	引物序列 Primer sequence （5’-3’）
CsCAMAT10	RT-CsCAMAT10-F	GCCATAGGAGCCTTAGCATA
CsCAMAT10	RT-CsCAMAT10-R	CCAGGGCAAACTCAACAACT
CsCAMAT9	RT-CsCAMAT9-F	TGATCCTTTGGTGCAGAACG
CsCAMAT9	RT-CsCAMAT9-R	CCAATCCAGCAACCATGTGA
CsCAMAT8	RT-CsCAMAT8-F	AGCATAATCTGGTCCGTTGG
CsCAMAT8	RT-CsCAMAT8-R	CGTTCTTCTGTTTGCTTTCG
CsCAMAT7	RT-CsCAMAT7-F	GCCATAACTGGAGGAAGAAG
CsCAMAT7	RT-CsCAMAT7-R	ATAGCTTCGCCTCTGGAAAT
CsCAMAT6	RT-CsCAMAT6-F	GCTGCTCGCATTCATCAAGT
CsCAMAT6	RT-CsCAMAT6-R	GAATCCACGCAAACCACTCC
CsCAMAT5	RT-CsCAMAT5-F	GTCCGCAGATGTTACTGGCTAC
CsCAMAT5	RT-CsCAMAT5-R	GCACTCAACTTGCTGGGATC
CsCAMAT4	RT-CsCAMAT4-F	AGGATGGCAAGACCGTCAAA
CsCAMAT4	RT-CsCAMAT4-R	GTTCAAGGGCAACCGCACCA
CsCAMAT3	RT-CsCAMAT3-F	GTTCTCCCGTCACTCCTGTC
CsCAMAT3	RT-CsCAMAT3-R	CCCTTCACGCTGATGATGTA
CsCAMAT2	RT-CsCAMAT2-F	TATGTTGTCTGGCTCCTCCC
CsCAMAT2	RT-CsCAMAT2-R	CGGCTGATTACATCCTCCTC
CsCAMAT1	RT-CsCAMAT1-F	GGAAATTGTGAAATGGGCAGTC
CsCAMAT1	RT-CsCAMAT1-R	TCCTCATCGTCATCGGCATC
CsGAPDH	RT-CsGAPDH-F	TTGGCATCGTTGAGGGTCT
CsGAPDH	RT-CsGAPDH-R	CAGTGGGAACACGGAAAGC

基因名称 Gene name	基因ID Gene ID	氨基酸 Amino acid/aa	分子量 Molecular weight/kD	等电点 pI	亚细胞定位Subcellular prediction	总平均疏水性Grand average hydropathicity	信号肽预测 Singal petides
CsCAMTA1	TEA005634	124	14.29	9.811	细胞核Nucleus	-0.388	无No
CsCAMTA2	TEA006574	984	109.83	6.153	细胞核Nucleus	-0.518	无No
CsCAMTA3	TEA011186	953	107.62	6.828	细胞核Nucleus	-0.475	无No
CsCAMTA4	TEA011489	155	17.63	9.515	细胞核Nucleus	-0.484	无No
CsCAMTA5	TEA011490	864	97.26	6.028	细胞核Nucleus	-0.401	无No
CsCAMTA6	TEA012477	1 419	157.99	6.669	细胞核Nucleus	-0.346	无No
CsCAMTA7	TEA015813	139	16.33	8.722	细胞核Nucleus	-0.637	无No
CsCAMTA8	TEA015818	970	108.43	4.976	细胞核Nucleus	-0.587	无No
CsCAMTA9	TEA025813	1 028	114.80	6.326	细胞核Nucleus	-0.512	无No
CsCAMTA10	TEA028584	325	36.55	9.820	细胞核Nucleus	0.006	无No

基因名称 Gene name	基因ID Gene ID	氨基酸 Amino acid/aa	分子量 Molecular weight/kD	等电点 pI	亚细胞定位Subcellular prediction	总平均疏水性Grand average hydropathicity	信号肽预测 Singal petides
CsCAMTA1	TEA005634	124	14.29	9.811	细胞核Nucleus	-0.388	无No
CsCAMTA2	TEA006574	984	109.83	6.153	细胞核Nucleus	-0.518	无No
CsCAMTA3	TEA011186	953	107.62	6.828	细胞核Nucleus	-0.475	无No
CsCAMTA4	TEA011489	155	17.63	9.515	细胞核Nucleus	-0.484	无No
CsCAMTA5	TEA011490	864	97.26	6.028	细胞核Nucleus	-0.401	无No
CsCAMTA6	TEA012477	1 419	157.99	6.669	细胞核Nucleus	-0.346	无No
CsCAMTA7	TEA015813	139	16.33	8.722	细胞核Nucleus	-0.637	无No
CsCAMTA8	TEA015818	970	108.43	4.976	细胞核Nucleus	-0.587	无No
CsCAMTA9	TEA025813	1 028	114.80	6.326	细胞核Nucleus	-0.512	无No
CsCAMTA10	TEA028584	325	36.55	9.820	细胞核Nucleus	0.006	无No

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