苜蓿绿肥对塔里木盆地苹果园土壤细菌多样性和功能的影响

doi:10.13304/j.nykjdb.2022.0942

中国农业科技导报 ›› 2024, Vol. 26 ›› Issue (8): 223-233.DOI: 10.13304/j.nykjdb.2022.0942

• 生物制造资源生态 • 上一篇

苜蓿绿肥对塔里木盆地苹果园土壤细菌多样性和功能的影响

刘霏霏¹(), 何万荣¹, 孙强², 席琳乔¹(), 廖结安³, 韩路⁴

^1.塔里木大学，新疆生产建设兵团塔里木畜牧科技重点实验室，新疆阿拉尔 843300
^2.新疆维吾尔自治区草原总站，乌鲁木齐 830049
^3.塔里木大学机械电气化工程学院，新疆阿拉尔 843300
^4.塔里木大学农学院，新疆阿拉尔，843300

收稿日期:2022-11-01 接受日期:2023-02-21 出版日期:2024-08-15 发布日期:2024-08-12
通讯作者: 席琳乔
作者简介:刘霏霏 E-mail：13239995801@163.com；
基金资助:
新疆生产建设兵团科技攻关项目(2018AB037)

Effect of Alfalfa Green Manure on Diversity and Function of Soil Bacteria in Apple Orchards in Tarim Basin

Feifei LIU¹(), Wanrong HE¹, Qiang SUN², Linqiao XI¹(), Jiean LIAO³, Lu HAN⁴

^1.Key Laboratory of Animal Science and Technology，Xinjiang Production and Construction Corps； Tarim University，Xinjiang Alar 843300，China
^2.Xinjiang Grassland Station，Urumqi 830049，China
^3.College of Mechanical and Electrical Engineering，Tarim University，Xinjiang Alar 843300，China
^4.College of Agricultural，Tarim University，Xinjiang Alar 843300，China

Received:2022-11-01 Accepted:2023-02-21 Online:2024-08-15 Published:2024-08-12
Contact: Linqiao XI

摘要/Abstract

摘要：

为探讨南疆干旱荒漠区苹果园种植覆盖作物苜蓿对果园土壤细菌群落结构与功能的影响，以苹果园间作苜蓿绿肥为处理（GMA）、清耕（CT）为对照，分别对0—20、20—40、40—60 cm土壤微生物16S rDNA 基因组采用Illumina MiSeq高通量测序技术和PICRUSt基因预测分析，比较不同处理下果园不同耕层土壤细菌群落结构、多样性及代谢功能变化。结果表明，苹果园间作苜蓿绿肥显著影响园区土壤细菌群落多样性，细菌拷贝数、丰富度指数（Chao1、ACE指数）及多样性指数（Shannon指数）均显著提高。其中，GMA处理下变形菌门（Proteobacteria）、放线菌门（Actinobacteria）和拟杆菌门（Bacteroidetes）的相对丰度分别为10.15%、64.97%和82.31%，较CT显著提高。通过PICRUSt功能预测，间作苜蓿绿肥明显提高了果园0—20 cm土层细菌功能的相对丰度，其中在7和9月提升最为显著，主要提高了土壤细菌对异物的生物降解能力，增强了土壤碳代谢、氮代谢相关功能菌的丰度。相关性分析表明，土壤细菌优势菌门与土壤全氮、总碳、碱解氮和有效钾含量呈显著正相关，与pH呈显著负相关。综上所述，苹果园种植苜蓿绿肥能显著改善土壤微生物群落组成，提高土壤细菌丰富度、多样性和拷贝数，显著提高土壤微生物功能，对果园土壤熟化、促进物质代谢及培肥地力具有重要作用。

关键词: 苜蓿, 覆盖作物, 细菌, 高通量测序, 功能预测

Abstract:

To explore the effect of planting cover crop alfalfa on the structure and function of soil bacterial community in apple orchards in the arid desert area of southern Xinjiang， the apple orchard intercropping alfalfa green manure （GMA） was as treatment with clear tillage （CT） as control， and 16S rDNA gene Illumina MiSeq high-throughput sequencing technology and PICRUSt were used analyzed the structure and function of soil bacterial community in 0—20， 20—40 and 40—60 cm soil， respectively. The bacterial community structure， diversity and metabolic function were compared between GMA and CT treatments. The results showed that cover crop alfalfa could significantly affect the diversity of soil bacterial community in the orchards， and the bacterial copy number and richness index （Chao1 and ACE index）， diversity index （Shannon index） had been significantly improved. The relative abundances of Proteobacteria， Actinobacteria and Bacteroidetes in GMA treatment significantly increased than CT treatment， with 10.15%， 64.97% and 82.31%， respectively. According to PICRUST function prediction， cover crop alfalfa could significantly increase the relative abundance of bacterial functions in 0—20 cm soil layer of the orchard， with the more significant increase ranges in July and September， which could improve the ability of soil bacteria to biodegrade foreign matter and increase soil carbon and nitrogen metabolism. The correlation analysis between soil factors and microbial diversity indicated that the dominant bacteria plylums were positively correlated with total nitrogen， total carbon， available nitrogen and available potassium， and negatively correlated with pH. In conclusion， cover crop alfalfa in apple orchard could significantly change the composition of soil microbial communities， increase soil bacterial richness， diversity and copy number， significantly improved soil microbial functions， and played an important role in orchard soil maturation， promotion of material metabolism and fertility.

Key words: alfalfa, cover crop, bacteria, high-throughput sequencing, functional prediction

中图分类号:

S661.1

刘霏霏, 何万荣, 孙强, 席琳乔, 廖结安, 韩路. 苜蓿绿肥对塔里木盆地苹果园土壤细菌多样性和功能的影响[J]. 中国农业科技导报, 2024, 26(8): 223-233.

Feifei LIU, Wanrong HE, Qiang SUN, Linqiao XI, Jiean LIAO, Lu HAN. Effect of Alfalfa Green Manure on Diversity and Function of Soil Bacteria in Apple Orchards in Tarim Basin[J]. Journal of Agricultural Science and Technology, 2024, 26(8): 223-233.

图/表 9

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指标Index	5月May		7月July		9月September
指标Index	CT	GMA	CT	GMA	CT	GMA
总碳含量 TC content/（g·kg^-1）	2.97±0.31 a	3.10±0.30 a	2.72±0.40 a	2.90±0.39 a	2.78±0.24 a	2.88±0.25 a
全氮含量 TN content/（g·kg^-1）	0.85±0.06 ab	0.91±0.07 ab	0.80±0.05 b	0.91±0.04 a	0.81±0.06 ab	0.91±0.07 ab
碱解氮含量 AN content/（mg·kg^-1）	38.92±9.82 a	30.33±6.92 a	4.78±4.77 b	5.40±2.40 b	1.15±0.19 b	1.58±0.70 b
速效磷含量 AP content/（mg·kg^-1）	9.91±3.97 b	14.57±2.32 ab	24.20±7.87 a	20.65±3.58 ab	23.72±2.49 a	21.95±0.59 a
速效钾含量 AK content/（mg·kg^-1）	136.77±38.50 a	155.03±42.80 a	123.67±6.28 a	99.40±46.35 a	120.27±45.91 a	83.70±22.40 a
pH	8.18±0.08 a	7.91±0.05 c	8.03±0.01 b	7.94±0.05 c	7.91±0.05 c	7.81±0.02 d
总盐含量 TS content/%	0.35±0.15 b	0.86±0.18 a	0.86±0.18 a	0.93±0.16 a	0.67±0.19 ab	0.86±0.33 a

指标Index	5月May		7月July		9月September
指标Index	CT	GMA	CT	GMA	CT	GMA
总碳含量 TC content/（g·kg^-1）	2.97±0.31 a	3.10±0.30 a	2.72±0.40 a	2.90±0.39 a	2.78±0.24 a	2.88±0.25 a
全氮含量 TN content/（g·kg^-1）	0.85±0.06 ab	0.91±0.07 ab	0.80±0.05 b	0.91±0.04 a	0.81±0.06 ab	0.91±0.07 ab
碱解氮含量 AN content/（mg·kg^-1）	38.92±9.82 a	30.33±6.92 a	4.78±4.77 b	5.40±2.40 b	1.15±0.19 b	1.58±0.70 b
速效磷含量 AP content/（mg·kg^-1）	9.91±3.97 b	14.57±2.32 ab	24.20±7.87 a	20.65±3.58 ab	23.72±2.49 a	21.95±0.59 a
速效钾含量 AK content/（mg·kg^-1）	136.77±38.50 a	155.03±42.80 a	123.67±6.28 a	99.40±46.35 a	120.27±45.91 a	83.70±22.40 a
pH	8.18±0.08 a	7.91±0.05 c	8.03±0.01 b	7.94±0.05 c	7.91±0.05 c	7.81±0.02 d
总盐含量 TS content/%	0.35±0.15 b	0.86±0.18 a	0.86±0.18 a	0.93±0.16 a	0.67±0.19 ab	0.86±0.33 a

土层 Soil layer/cm	处理 Treatment	5月 May	7月 July	9月 September
0—20	CT	1.36±0.27 b	3.01±0.24 b	2.80±0.30 b
0—20	GMA	3.09±0.45 a	9.45±1.29 a	6.96±0.85 a
20—40	CT	0.98±0.16 bc	1.35±0.17 c	1.31±0.12 c
20—40	GMA	1.61±0.33 b	2.91±0.35 b	1.28±0.17 c
40—60	CT	0.76±0.16 c	0.92±0.21 c	1.00±0.14 c
40—60	GMA	1.03±0.23 bc	0.95±0.34 c	0.91±0.25 c

土层 Soil layer/cm	处理 Treatment	5月 May	7月 July	9月 September
0—20	CT	1.36±0.27 b	3.01±0.24 b	2.80±0.30 b
0—20	GMA	3.09±0.45 a	9.45±1.29 a	6.96±0.85 a
20—40	CT	0.98±0.16 bc	1.35±0.17 c	1.31±0.12 c
20—40	GMA	1.61±0.33 b	2.91±0.35 b	1.28±0.17 c
40—60	CT	0.76±0.16 c	0.92±0.21 c	1.00±0.14 c
40—60	GMA	1.03±0.23 bc	0.95±0.34 c	0.91±0.25 c

处理 Treatment	月份 Month	操作分类单元数 Number of OTUs	Chao1指数 Chao1 index	ACE指数 ACE index	Shannon指数 Shannon index	Simpson指数 Simpson index	Coverage指数 Coverage index
CT	5	5 585	7 076.7±30.0 ab	7 092.5±85.9 ab	6.981 7±0.089 6 ab	0.003 1±0.000 4 a	0.985 2±0.000 4 ab
	7	5 228	6 587.7±344.0 ab	6 547.8±304.4 ab	6.868 6±0.169 6 ab	0.005 3±0.004 5 a	0.986 7±0.000 7 ab
	9	5 203	6 494.3±55.0 b	6 492.4±58.4 b	6.917 4±0.092 0 ab	0.003 9±0.001 9 a	0.986 9±0.000 2 a
GMA	5	5 702	7 228.7±639.2 a	7 235.8±638.6 a	7.069 8±0.070 2 a	0.002 4±0.000 2 a	0.985 0±0.001 6 b
	7	5 526	7 037.7±493.8 ab	6 985.4±438.0 ab	7.071 7±0.062 0 a	0.002 4±0.000 2 a	0.985 6±0.001 0 ab
	9	5 185	6 624.7±36.0 ab	6 628.0±102.7 ab	6.848 6±0.071 5 b	0.003 4±0.000 6 a	0.986 2±0.000 2 ab

苜蓿绿肥对塔里木盆地苹果园土壤细菌多样性和功能的影响

Effect of Alfalfa Green Manure on Diversity and Function of Soil Bacteria in Apple Orchards in Tarim Basin

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