中国农业科技导报 ›› 2025, Vol. 27 ›› Issue (3): 71-82.DOI: 10.13304/j.nykjdb.2023.0591

• 生物技术 生命科学 • 上一篇    下一篇

茶树钙调蛋白结合转录激活因子(CAMTA)家族基因鉴定与表达分析

刘金仙1,2(), 王丽娟2(), 刘杰2, 傅仙玉1,2, 武广珩3   

  1. 1.中国乌龙茶产业协同创新中心,福建 武夷山 354300
    2.武夷学院茶与食品学院,福建 武夷山 354300
    3.福建省生态产业绿色技术重点实验室,福建 武夷山 354300
  • 收稿日期:2023-08-08 接受日期:2023-10-18 出版日期:2025-03-15 发布日期:2025-03-14
  • 通讯作者: 刘金仙
  • 作者简介:刘金仙 E-mail:liujinxian@163.com
    王丽娟 E-mail:w892694385@163.com第一联系人:刘金仙和王丽娟为共同第一作者。
  • 基金资助:
    福建省自然科学基金项目(2022J011200);福建省科技计划引导性项目(2023N0017);资源化学产业科技创新联合项目(N2021Z006)

Identification and Expression Analysis of Calmodulin-binding Transcription Activator (CAMTA) Family Genes in Tea Plants

Jinxian LIU1,2(), Lijuan WANG2(), Jie LIU2, Xianyu FU1,2, Guangheng WU3   

  1. 1.Collaborative Innovation Center of Chinese Oolong Tea Industry,Fujian Wuyishan 354300,China
    2.College of Tea and Food,Wuyi University,Fujian Wuyishan 354300,China
    3.Fujian Provincial Key Laboratory of Eco-industrial Green Technology,Fujian Wuyishan 354300,China
  • Received:2023-08-08 Accepted:2023-10-18 Online:2025-03-15 Published:2025-03-14
  • Contact: Jinxian LIU

摘要:

为了解茶树钙调蛋白结合转录激活因子(calmodulin-binding transcription activator,CAMTA家族成员的功能,探究其表达特性,利用生物信息学方法在茶树全基因组中鉴定CsCAMTAs基因,并对其理化性质、基因结构、结构域、保守基序、系统进化、顺式作用元件进行分析,利用qRT-PCR技术分析CsCAMTAs在不同组织、不同激素处理和病菌胁迫下的表达情况。结果表明,在茶树全基因组中共鉴定出10个CsCAMTAs成员,共包含10个motifs,分布在6条染色体,均定位于细胞核。不同CsCAMTAs基因差异较大,氨基酸数目为124~1 419个,编码蛋白等电点为4.976~9.820,除1个为弱疏水蛋白外,其他均为亲水蛋白。系统进化分析将茶树10个CsCAMTAs基因划分为4个类群,有4个同源基因对,同源基因对在基因结构、保守结构域、保守基序数量和相对位置等方面具有较高相似性。CsCAMTAs启动子区含有34个与光响应、激素诱导以及生物胁迫相关的顺式作用元件。表达分析结果显示,CsCAMTAs在不同组织的表达有明显差异,其中CsCAMTA1、3、4、7、8、10在老叶中的表达水平较高;除CsCAMTA5不受茉莉酸甲酯诱导外,其他CsCAMTAs基因均受茉莉酸甲酯、脱落酸、水杨酸及茶轮斑病菌诱导表达,由此说明CsCAMTAs不仅调控茶树生长发育,还参与茶树对病菌和激素的应答。

关键词: 茶树, 钙调蛋白结合转录激活因子(CAMTA), 生物信息学分析, 表达分析

Abstract:

In order to understand calmodulin-binding transcription activator (CAMTA) family members in tea plants and explore their expression characteristics, the CsCAMTAs were identified in the whole genome of tea plants, and their physicochemical properties, gene structure, domain, conserved motif order, phylogenetic relationship and cis-acting elements were analyzed by bioinformatics method, and the expression of CsCAMTAs in different tissues, under different hormone treatments and bacterial stress were analyzed by qRT-PCR. The results showed that a total of 10 CsCAMTAs members were identified with 10 motifs, which distributed in 6 chromosomes and all localized in the nucleus. The sizes of different CsCAMTAs gene were different with the number of amino acids from 124 to 1 419, the isoelectric points of the coding protein were from 4.976 to 9.820, and only 1 was weak hydrophobic protein, the others were hydrophilic proteins. Phylogenetic analysis showed that the CsCAMTAs were clustered into 4 groups, with 4 homologous gene pairs, and the homologous gene pairs had high similarity in gene structure, conserved domain, number of conserved motifs and relative position. The promoter region of CsCAMTAs contained 34 cis-acting elements associated with light response, hormone induction, and biotic stress. The expression analysis results showed that the expressions of CsCAMTAs in different tissues were significantly different. CsCAMTA13,47810 had higher expression level in mature leaves. Except for CsCAMTA5, which was not induced by methyl jasmonate, the others were induced by methyl jasmonate, abscisic acid, salicylic acid and tea wheel spot pathogen, which indicated that CsCAMTAs not only participated in the regulation of tea plants growth and development, but also participated in the response of tea plants to bacteria and hormones.

Key words: tea plants, calmodulin-binding transcriptional activator (CAMTA), bioinformatics analysis, expression analysis

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