Study on Extraction Process and Antibacterial Activity of Lignans from Cinnamomum camphora Leaves

doi:10.13304/j.nykjdb.2023.0281

Abstract

Abstract:

To study the optimum reflux extraction process and antifungal activity of total lignans from Cinnamomum camphora leaves， the leaves of C. camphora were as material， and the contents of sesamin and 9-hydroxysesamin in C. camphora leaves were determined. The reflux extraction process of total lignans from C. camphora was optimized by single factor combined with Box-Behnken response surface method， and the antifungal activity of total lignans was evaluated by toxic plate method. The results showed that the optimum conditions of reflux extraction were as follows： extraction temperature 86 ℃， ratio of solid to liquid 1∶8.39 （g·mL^-1）， extraction time 43 min and anhydrous ethanol as extraction solvent. Under these conditions， the extraction rate of lignans was 0.380 4%. The influence of factors on the extraction rate was extraction temperature>extraction time>solid-liquid ratio. The antifungal activity test showed that the antifungal effects of lignans on Gliocladium catenulatum， Valsa mali Miyabeet Yamada， Fusarium oxysporumf and Pythium aphanidermatum were the best with 2 mg·mL^-1， and the antifungal rates were more than 50%. The EC₅₀ values were 1.238， 1.280， 1.486 and 0.419 mg·mL^-1， respectively. Above results provided theoretical references for the subsequent development and utilization of C. camphora leaves and the research and development of plant-derived pesticides.

Key words: Cinnamomum camphora, lignan, response surface method, antibacterial activity, toxicity

摘要：

为研究樟叶总木脂素的最佳回流提取工艺及抑菌活性，以樟叶为研究对象，测定其芝麻素和9-羟基芝麻素含量，通过单因素联合Box-Behnken响应面法对樟叶总木脂素回流提取工艺进行优化，并采用带毒平板法对总木脂素的抑菌活性进行评价。结果表明，回流提取最佳工艺条件为提取温度86 ℃、料液比1∶8.39（g·mL^-1）、提取时间43 min、提取溶剂为无水乙醇，在此条件下木脂素提取率达0.380 4%。各因素对提取率的影响表现为提取温度>提取时间>料液比。抑菌活性试验表明，木脂素含量为2 mg·mL^-1时对链孢粘帚霉菌（Gliocladium catenulatum）、苹果黑腐皮壳菌（Valsa mali Miyabeet Yamada）、西瓜尖孢镰孢菌（Fusarium oxysporumf）和瓜果腐霉菌（Pythium aphanidermatum）的抑菌效果最佳，抑菌率均达到50%以上；有效中质量浓度（EC₅₀）分别为1.238、1.280、1.486和0.419 mg·mL^-1。以上研究结果为樟叶开发利用及植物源农药研究及开发提供了理论参考。

关键词: 樟, 木脂素, 响应面法, 抑菌活性, 毒力

CLC Number:

S792.23

Lanxiong ZHANG, Wei ZHENG, Yuan’an CHEN, Jing SHEN, Shuangquan ZOU, Jianrong WU, Lin NI. Study on Extraction Process and Antibacterial Activity of Lignans from Cinnamomum camphora Leaves[J]. Journal of Agricultural Science and Technology, 2024, 26(5): 138-147.

张岚雄, 郑威, 陈源桉, 沈婧, 邹双全, 伍建榕, 倪林. 樟叶总木脂素提取工艺及其抑菌活性研究[J]. 中国农业科技导报, 2024, 26(5): 138-147.

Figures/Tables 12

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菌株Strain	名称Name	类别Taxonomy
F-3	西瓜尖孢镰孢菌Fusarium oxysporumf	半知菌类Deuteromycetes
G-1	链孢粘帚霉菌Gliocladium catenulatum	半知菌类Deuteromycetes
V	苹果黑腐皮壳菌Valsa mali Miyabe et Yamada	子囊菌门Ascomycota
P-3	瓜果腐霉菌Pythium aphanidermatum	卵菌门Oomycetes

菌株Strain	名称Name	类别Taxonomy
F-3	西瓜尖孢镰孢菌Fusarium oxysporumf	半知菌类Deuteromycetes
G-1	链孢粘帚霉菌Gliocladium catenulatum	半知菌类Deuteromycetes
V	苹果黑腐皮壳菌Valsa mali Miyabe et Yamada	子囊菌门Ascomycota
P-3	瓜果腐霉菌Pythium aphanidermatum	卵菌门Oomycetes

水平level	因素Factor
水平level	A：提取温度 Extraction temperature/℃	B：料液比 Ratio of solid to liquid/（g·mL^-1）	C：提取时间 Extraction time/min
-1	80	1∶6	30
0	85	1∶8	40
1	90	1∶10	50

水平level	因素Factor
水平level	A：提取温度 Extraction temperature/℃	B：料液比 Ratio of solid to liquid/（g·mL^-1）	C：提取时间 Extraction time/min
-1	80	1∶6	30
0	85	1∶8	40
1	90	1∶10	50

方差来源 Source of variance	平方和 Sum of squares	自由度 df	均方 Mean square	F值 F value	P值 P value
模型 Model	1.136×10^-3	9	1.262×10^-4	52.72	<0.000 1
A	1.271×10^-4	1	1.271×10^-4	53.06	0.000 2
B	5.544×10^-5	1	5.544×10^-5	23.15	0.001 9
C	1.090×10^-4	1	1.090×10^-4	45.53	0.000 3
AB	2.721×10^-5	1	2.721×10^-5	11.36	0.011 9
AC	3.978×10^-6	1	3.978×10^-6	1.66	0.238 4
BC	5.067×10^-6	1	5.067×10^-6	2.12	0.189 1
A²	4.009×10^-4	1	4.009×10^-4	167.42	<0.000 1
B²	2.196×10^-4	1	2.196×10^-4	91.72	<0.000 1
C²	1.096×10^-4	1	1.096×10^-4	45.79	0.000 3
残差 Residual error	1.676×10^-5	7	2.395×10^-6
失拟项 Lack of fit	9.321×10^-6	3	3.107×10^-6	1.67	0.309 2
纯误差 Pure error	7.440×10^-6	4	1.860×10^-6
总和 Summation	1.153×10^-3	16