Abstract:

JAZ protein and MYC2-like transcription factor， which act as key regulators of the jasmonate （JA） signal transduction pathway， play important roles on plant growth and development and specialized metabolisms. Using the amino acid sequences of 13 JAZ proteins and 11 MYC2-like proteins from Arabidopsis thaliana as reference sequences， the LaJAZ and LaMYC2-like genes in the Lavandula angustifolia genome were retrieved and subjected to bioinformatics analysis. The results showed that a total of 26 LaJAZ and 26 LaMYC2-like genes were identified in whole genome of Lavandula angustifolia， which were unevenly distributed on 22 chromosomes. The promoter cis-acting elements of LaJAZ and LaMYC2-like genes involved plant responses to light， jasmonate， abscisic acid， defense and stress reactions. qRT-PCR showed that the selected 6 LaJAZ genes had higher express level in deep purple sepals， while the selected LaMYC2-like genes had higher express level in light purple sepals. In addition， 15 LaJAZ and LaMYC2-like genes were induced by methyl jasmonate （MeJA）. Above results laid the foundation for the functional analysis of LaJAZ and LaMYC2-like genes in Lavandula angustifolia and their response mechanism to JA signaling.

Key words: Lavandula angustifolia, JAZ family, MYC2-like family, bioinformatics, expression pattern, MeJA

摘要：

JAZ蛋白和MYC2-like转录因子是茉莉酸（jasmonate，JA）类物质信号转导途径的关键调节因子，在植物的生长发育和次级代谢中发挥重要作用。以拟南芥13个JAZ蛋白和11个MYC2-like蛋白的氨基酸序列为参考序列，检索薰衣草基因组中的LaJAZ和LaMYC2-like基因家族成员，并对其进行生物信息学分析。结果表明，在薰衣草基因组中共鉴定到26个LaJAZ和26个LaMYC2-like基因，这些基因不均匀的分布在22条染色体上。LaJAZ和LaMYC2-like基因启动子的顺式作用元件涉及植物对光、茉莉酸、脱落酸、防御和胁迫反应的响应。qRT-PCR显示，选择的6个LaJAZ基因在深紫色萼片中表达较高，而LaMYC2-like基因在浅紫色萼片中表达较高。此外，有15个LaJAZ和LaMYC2-like基因受茉莉酸甲酯（methyl jasmonate，MeJA）诱导。以上研究结果为薰衣草LaJAZ和LaMYC2-like基因的功能分析及其对JA信号的响应机理研究奠定了基础。

关键词: 薰衣草, JAZ家族, MYC2-like家族, 生物信息学, 表达模式, MeJA