Journal of Agricultural Science and Technology ›› 2024, Vol. 26 ›› Issue (2): 46-55.DOI: 10.13304/j.nykjdb.2022.0818

• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles     Next Articles

Development and Effect Evaluation of KASP Markers for Fiber Strength in Gossypium hirsutum L.

Lihua LI(), Zhengwen SUN, Huifeng KE, Qishen GU, Liqiang WU, Yan ZHANG, Guiyin ZHANG, Xingfen WANG()   

  1. State Key Laboratory of North China Crop Improvement and Regulation,Key Laboratory for Crop Germplasm Resources of Hebei,College of Agronomy,Hebei Agricultural University,Hebei Baoding 071000,China
  • Received:2022-09-25 Accepted:2022-10-18 Online:2024-02-15 Published:2024-02-04
  • Contact: Xingfen WANG

陆地棉纤维强度KASP-SNP标记的开发及效应评价

李丽花(), 孙正文, 柯会锋, 谷淇深, 吴立强, 张艳, 张桂寅, 王省芬()   

  1. 河北农业大学农学院,华北作物改良与调控国家重点实验室,河北省作物种质资源重点实验室,河北 保定 071000
  • 通讯作者: 王省芬
  • 作者简介:李丽花 E-mail:13473229312@163.com
  • 基金资助:
    国家现代农业产业技术体系建设项目(CARS-15-03);河北省重点研发计划项目(21326314D)

Abstract:

Fiber strength (FS) is an important indicator of fiber quality traits, and developing molecular markers associated with target traits would improve the selection accuracy and accelerate the process for breeding. The selected KASP markers were genotyped among 376 varieties (lines), and then the polymorphism and the efficacy for higher strength were analyzed. 3 SNP markers showed polymorphic in the population, and the selection rate of FS-15 for high-strength fiber was 63.2% while FS-16 and FS-29 were 60.0% and 56.1%, respectively. Furthermore, the evaluation efficacy of different haplotype combination (Hap1, Hap2, Hap3 and Hap4) suggested that the accessions harboring Hap3 (TTA) showed higher fiber strength than those harboring other haplotypes. The selection rate of Hap3 for higher strength was 72.7%. In addition, Hap3 had no adverse effect on fiber length, boll weigh, seed index and lint index. The above results indicated these KASP markers and Hap3 could be used for selection of higher strength materials by providing genotypic information and did not adversely affect the fiber length, boll weight, seed index and lint index.

Key words: cotton, fiber strength, KASP-SNP markers, haplotype combination, effect evaluation

摘要:

纤维强度(fiber strength,FS)是判断棉花纤维品质优劣的重要指标之一,开发可用于目标性状选择的分子标记将会大大提高选择准确性并加快育种进程。设计KASP引物,对国内外的376份品种(系)进行基因型鉴定,筛选多态性标记并验证其对高强纤维材料选择的有效性。3个SNP位点在材料中均具有多态性,标记FS-15对高强纤维材料的选择率为63.2%,FS-16和FS-29分别为60.0%和56.1%。对单倍型组合(Hap1、Hap2、Hap3和Hap4)的有效性评价表明,携带Hap3(TTA)的材料纤维强度显著高于其他组合,对高强纤维材料选择率达72.7%。此外,Hap3对纤维长度、铃重、籽指和衣指无不利影响。以上结果表明,开发的KASP标记及Hap3可用于高纤维强度材料的辅助选择,同时未对纤维长度、铃重、籽指和衣指等性状产生不利影响。

关键词: 棉花, 纤维强度, KASP-SNP标记, 单倍型组合, 效应评价

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