Effects of Biocontrol Bacteria on Soil Properties and Bacterial Community Structure of Ginseng Continuous Cropping

doi:10.13304/j.nykjdb.2023.0907

Abstract

Abstract:

In order to investigate the effect of biocontrol bacterial on the microecology of ginseng continuous cropping soil， a biocontrol bacterial treatment experiment was conducted in the field. Chemical analysis and high-throughput sequencing technology were used to study the changes in soil physicochemical properties， soil enzyme activities， and bacterial community. The results showed that the application of biocontrol bacteria significantly decreased soil electric conductivity， and increased soil pH， the contents of organic matter， available nitrogen， available phosphorus and the activities of soil urease， soil sucrase， and soil acid phosphatase， with an increase of 9.00% in soil organic matter content and an increase of 19.42% in soil sucrase activity. Bacterial taxonomy analysis divided soil bacterial communities into 39 phyla， 124 classes， 279 orders， 424 families， and 774 genera. Biocontrol bacteria significantly increased the ACE index and Chao index of soil bacteria. Correlation analysis showed that pH， organic matter， available nitrogen， sucrase， urease were significantly positively correlated with Arthrobacter， and significantly negatively correlated with Bradyrhizobium and Microlunatus； available phosphorus content was significantly positively correlated with Candidatus_ Xiphinemabacter， RB41， and significantly negatively correlated with Candidatus_Udaeobacter， Arthrobacter； and there was significantly negative correlation between acid phosphatase and Mycobacterium. In summary， biocontrol bacteria stimulated the growth and reproduction of beneficial microorganisms in soil， increased the fertility and enzyme activity of the soil， and improved the soil nutritional status. Therefore， biocontrol bacteria should be expected to become an effective way to repair degraded soil.

Key words: biocontrol bacteria, ginseng, physical and chemical properties, enzyme activities, bacterial structure

摘要：

为探究生防细菌对人参连作土壤微生态的影响，在田间进行生防细菌处理试验。采用化学分析及高通量测序技术研究土壤理化性质、酶活性和细菌群落的变化。结果表明，施用生防细菌后，土壤电导率显著降低；土壤pH、有机质、水解氮和速效磷含量及土壤脲酶、蔗糖酶、酸性磷酸酶活性显著提高，其中土壤有机质含量增加9.00%；土壤蔗糖酶活性增加19.42%。细菌分类学分析将土壤细菌群落划分为39门、124纲、279目、424科、774属，生防细菌处理显著提高了土壤细菌的ACE指数和Chao指数。相关性分析表明，土壤pH、有机质、水解氮、蔗糖酶、脲酶与节杆菌属（Arthrobacter）呈显著正相关，与慢生根瘤菌属（Bradyrhizobium）、小月菌属（Microlunatus）呈显著负相关；土壤速效磷含量与Candidatus_Xiphinematobacter、RB41 呈显著正相关，与Candidatus_Udaeobacter、节杆菌属呈显著负相关；土壤酸性磷酸酶与分支杆菌属（Mycobacterium）呈显著负相关。综上所述，生防细菌刺激了土壤有益微生物的生长和繁殖，提高了人参连作土壤的肥力和土壤酶活性，改善了土壤营养状况，因此生防细菌有望成为修复退化土壤的有效途径。

关键词: 生防细菌, 人参, 理化性质, 酶活性, 细菌结构

CLC Number:

S154

Ergang WANG, Pengyuan LYU, Yi ZHOU, Yu ZHAN, Guixiang HE, Lixiang WANG, Xinyue MIAO, Changbao CHEN, Qiong LI. Effects of Biocontrol Bacteria on Soil Properties and Bacterial Community Structure of Ginseng Continuous Cropping[J]. Journal of Agricultural Science and Technology, 2025, 27(4): 140-148.

王二刚, 吕朋元, 周一, 战宇, 何贵祥, 王丽翔, 苗馨月, 陈长宝, 李琼. 生防细菌对人参连作土壤性质及细菌群落结构的影响[J]. 中国农业科技导报, 2025, 27(4): 140-148.

Figures/Tables 7

Table 1 Soil physical and chemical properties under different treatments

处理 Treatment	pH	电导率EC/（μS·cm^-1）	有机质OM/（g∙kg^-1）	碱解氮AN/（mg∙kg^-1）	有效磷AP/（mg∙kg^-1）
CK	6.38±0.03 c	118.00±3.78 a	33.76±1.40 c	315.25±4.22 c	6.27±0.18 b
SZ	6.62±0.02 b	103.85±1.36 b	36.80±0.93 b	325.28±4.75 b	6.89±0.14 a
XT	6.72±0.01 a	85.20±2.21 c	43.37±1.40 a	346.13±5.71 a	5.85±0.11 c

Fig. 1 Soil enzyme activities under different treatmentsNote： Different lowercase letters indicate significant differences between different treatments at P<0.05 level.

Table 2 Soil bacterial sequence and Alpha diversity index under different treatments

处理

Treatment

序列数

Raw read

OUT数量

Number of OTU

Shannon指数

Shannon index

Ace指数

Ace index

Chao指数

Chao index

Coverage

覆盖度

Fig. 2 Composition of soil bacterial communities under different treatmentsA： Phyla level； B： Genus level

Fig. 3 PCo analysis of soil bacterial communities under different treatments

Fig. 4 Discriminant analysis of multi-level species differences in bacterial LefSe under different treatmentsA： LEfSe multi-level species tree diagram； B： LDA discrimination bar chart

Fig. 5 Correlation analysis between soil bacterial genus and environmental factors under different treatmentsNote：*， ** and *** indicate significant correlations at P<0.05， P<0.01 and P<0.001 levels， respectively.

References 31

1	国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2020:8-9.
2	KUMAR J P, PARK E, FAQEERZADA M A, et al.. Analysis of RGB plant images to identify root rot disease in Korean ginseng plants using deep learning [J/OL]. Appl. Sci., 2022, 12(5):2489 [2023-11-20]. .
3	JI L, TIAN L, NASIR F, et al.. Impacts of replanting American ginseng on fungal assembly and abundance in response to disease outbreaks [J]. Arch. Microbiol. Sci., 2021, 203(5):2157-2170.
4	侯雨萱,于林,李阳,等.16种杀菌剂对3种水稻病原细菌的室内抑菌效果研究[J].中国稻米,2023,29(6):28-32.
	HOU Y X, YU L, LI Y, et al.. Indoor bacteriostatic effects of 16 fungicides against three pathogenic bacteria from rice [J]. China Rice, 2023, 29(6):28-32.
5	李焕,刘鸣韬,田雪亮,等.豫北地区设施蔬菜休闲期土壤杀菌剂残留特征[J].湖北农业科学,2018,57(4):56-59.
	LI H, LIU M T, TIAN X L, et al.. Characteristic of fungicide residue in greenhouse soil during fallow period in Northern [J]. Hubei Agric. Sci., 2018, 57(4):56-59.
6	BRODEUR J, ABRAM P, HEIMPEL G, et al.. Trends in biological control: public interest, international networking and research direction [J]. BioControl, 2018, 63(1):11-26.
7	DONG X, FANG L, YE Z, et al.. Screening of biocontrol bacteria against soft rot disease of Colocasia esculenta (L.) schott and its field application [J/OL]. PLoS One, 2021, 16(7):e0254070 [2023-11-20]. .
8	涂镜,魏宝阳,付威,等.生防菌对土壤微生物群落结构影响的研究进展[J].湖南农业科学,2023(6):96-100.
	TU J, WEI B Y, FU W, et al.. Research progress on the effect of biocontrol microorganisms on soil microbial community structure [J]. Hunan Agric. Sci., 2023(6):96-100.
9	程雪梅,李迪帅,蒋征,等.生防菌剂对浙贝母产量、质量及根际土壤环境的影响[J]. 中药材,2023,46(2):284-292.
	CHENG X M, LI D S, JIANG Z, et al.. Effects of biocontrol agents on yield, quality and rhizosphere soil environment of Fritillaria thunbergii [J]. J. Chin. Med. Mater., 2023, 46(2):284-292.
10	沈婷婷,张琇,杨国平,等.生防菌剂对西瓜根际土壤微生物群落和尖孢镰刀菌属的影响[J].西北农林科技大学学报(自然科学版),2023,51(7):115-125.
	SHEN T T, ZHANG X, YANG G P, et al.. Effects of biocontrol agents on watermelon rhizosphere soil microbial community and Fusarium oxysporum F. sp. [J]. J. Northwest A&F Univ. (Nat. Sci.), 2023, 51(7):115-125.
11	HUSSAIN H, KASINADHUNI N, ARIOLI T. The effect of seaweed extract on tomato plant growth, productivity and soil [J]. J. Appl. Phycol., 2021, 33(2):1305-1314.
12	CHEN P, WANG Y, QI Z L, et al.. Phase changes of continuous cropping obstacles in strawberry (Fragaria×ananassa Duch.) production [J/OL]. Appl. Soil Ecol., 2020, 155:103626 [2023-11-20]. .
13	GOLDFORD J, LU N, BAJIC D, et al.. Emergent simplicity in microbial community assembly [J]. Science, 2018, 361(6401):469-474.
14	鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000:1-495.
15	陈忠男,王志刚,徐伟慧.生防菌在农业中的应用及其机制研究进展[J].高师理科学刊,2022,42(6):89-94.
	CHEN Z N, WANG Z G, XU W H. Research progress of application and mechanism of biocontrol bacteria in agriculture [J]. J. Sci. Teachnol. Coll. Univ., 2022, 42(6):89-94.
16	YOU J F, LIU X, ZHANG B, et al.. Seasonal changes in soil acidity and related properties in ginseng artificial bed soils under a plastic shade [J]. J. Ginseng Res., 2015, 39(1):81-88.
17	简在友,王文全,孟丽,等.人参连作土壤元素含量分析[J].土壤通报,2011,42(2):369-371.
	JIAN Z Y, WANG W Q, MENG L, et al.. Analysis of element contents in soil for continuous cropping ginseng [J]. Chin. J. Soil Sci., 2011, 42(2):369-371.
18	SUN H, WANG Q X, LIU N, et al.. Effects of different leaf litters on the physicochemical properties and bacterial communities in Panax ginseng-growing soil [J]. Appl. Soil Ecol., 2017, 111:17-24.
19	RAWALD W. Activity of soil enzymes as a component of the soil biological activity, specially with regard to the judgement about soil fertility, and aspects of the soil enzymatic research [J]. Zentralblatt für Bakteriologie, Parasitenkunde, 1970, 125(4):363-384.
20	XU H W, QU Q, CHEN Y H, et al.. Responses of soil enzyme activity and soil organic carbon stability over time after cropland abandonment in different vegetation zones of the Loess Plateau of China [J/OL]. CATENA, 2021, 196:104812 [2023-11-20]. .
21	HE W X, MA A S, WU Y J, et al.. Effect of arsenic on soil urease activity [J]. Chin. J. Appl. Ecol., 2004, 15(5):895-898.
22	ANTONIOU S, GEORG E. Impact of soil management and two botanical insecticides on urease and invertase activity [J]. J. Environ. Sci. Health, 2003, 38(4):479-488.
23	高吉坤,孙雨薇,曹旭,等.生防枯草芽孢杆菌B29对黄瓜根际功能细菌及土壤酶活性的影响[J].安徽农业科学, 2015,43(10):107-110.
	GAO J K, SUN Y W, CAO X, et al.. Effects of Bacillus subtilis B29 on rhizosphere functional bacteria and soil enzymes of cucumber [J]. J. Anhui Agric. Sci., 2015, 43(10):107-110.
24	DEL VECCHIO H A, YING S, PARK J, et al.. The cell wall-targeted purple acid phosphatase AtPAP25 is critical for acclimation of Arabidopsis thaliana to nutritional phosphorus deprivation [J]. Plant J., 2014, 80(4):569-581.
25	余贤美,安淼,王海荣,等.枯草芽孢杆菌Bs-15对板栗土壤微生物种群数量及功能多样性的影响[J].生态环境学报,2014,23(4):598-602.
	YU X M, AN M, WANG H R, et al.. Influence of Bacillus subtilis Bs-15 on the microbial population and functional diversity of microbial communities in the chestnut soil [J]. Ecol. Environ. Sci., 2014, 23(4):598-602.
26	KIESER T, BIBB M J, BUTTNER M J, et al.. Practical Streptomyces genetics [M]. John Innes Foundation, Norwich, United Kingdom, 2000: 1-201.
27	DONG W, LIU E, YAN C, et al.. Impact of no tillage vs. conventional tillage on the soil bacterial community structure in a winter wheat cropping succession in northern China [J]. Eur. J. Soil. Biol., 2017, 80:35-42.
28	王娟娟,朱紫娟,钱晓晴,等.全年稻麦秸秆还田对稻田土壤细菌群落结构的影响[J].中国土壤与肥料, 2022(4):57-65.
	WANG J J, ZHU Z J, QIAN X Q, et al.. Effects of year-round rice-wheat straw return on soil bacterial community structure in paddy fields [J]. Soil Fert. Sci. China, 2022(4):57-65.
29	刘彩霞,董玉红,焦如珍.森林土壤中酸杆菌门多样性研究进展[J].世界林业研究, 2016,29(6):17-22.
	LIU C X, DONG Y H, JIAO R Z. Research progress in acidobacteria diversity in forest soil [J]. World For. Res., 2016, 29(6):17-22.
30	HEDLUND B P, GOSINK J J, STALEY J T. Verrucomicrobia div. nov., a new division of the bacteria containing three new species of Prosthecobacter [J]. Antonie Van Leeuwenhoek, 1997, 72(1):29-38.
31	王泽铭,李传虹,马巧丽,等.湿度盐度pH协同驱动锡林河景观疣微菌群空间异质性[J].微生物学报,2021,61(6):1728-1742.
	WANG Z M, LI C H, MA Q L, et al.. Moisture, salinity and pH co-driving spatial heterogeneity of Verrucomicrobial populations in Xilin river landscape [J]. Acta Microbiol. Sin., 2021, 61(6):1728-1742.

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