Genome-wide Identification and Tissue Expression Pattern Analysis of HSP70 Gene Family in Thinopyrum elongatum

doi:10.13304/j.nykjdb.2024.0409

Abstract

Abstract:

Heat shock protein 70 （HSP70） constitutes a highly conserved and potent protein family. However， the investigation of HSP70 in Thinopyrum elongatum has not been documented. To identify and study HSP70 gene family in Thinopyrum elongatum， TeHSP70 genes were identified based on the systematic application of bioinformatics methods and their expression patterns under salt stress and in different tissues were systematically analyzed. The results showed that a total of 29 TeHSP70 genes were identified in Thinopyrum elongatum， which was categorized into 3 subfamilies and evenly distributed across 7 chromosomes. There were 26 TeHSP70 genes localized in cytoplasm， and all 29 TeHSP70 genes included alpha-helix， extended strand， beta-turn and random coil. The TeHSP70s genes exhibited highly conserved motifs and diverse gene structures， and included multiple hormonal and stress-responsive cis-acting elements in upstream. The collinearity analysis revealed the presence of 3 collinear gene pairs， as well as tandem repeat and segment repeat events， within 29 TeHSP70 genes， and there were numerous homologous gene pairs among Thinopyrum elongatum， rice and wheat， whereas there was a scarcity of such pairs between Thinopyrum elongatum and Arabidopsis. Protein interaction network analysis revealed that there were 11 TeHSP70 genes with a complex and closely protein interaction network. Analysis of expression patterns showed that， with increasing of salt stress （0~300 mmol·L^-1）， 7 TeHSP70 genes exhibited continuous up-regulation and 6 TeHSP70 genes showed continuous down-regulation， while the remaining 16 members either did not show any expression or displayed inconsistent changes. Similarly， 25 TeHSP70 genes were expressed across all tissues， and 4 TeHSP70 genes were only expressed in some tissues. Above results provided effective references for further analysis of HSP70 genes function of Thinopyrum elongatum.

Key words: Thinopyrum elongatum, HSP70, salt stress, expression pattern

摘要：

热激蛋白HSP70（heat shock protein 70）家族是一类结构高度保守和功能强大的蛋白家族，然而有关其在二倍体长穗偃麦草（Thinopyrum elongatum）中的研究尚未报道。为研究二倍体长穗偃麦草HSP70基因家族（TeHSP70），基于生物信息学方法对二倍体长穗偃麦草TeHSP70基因进行鉴定，并系统分析其在盐胁迫和多组织中的表达模式。结果表明，在二倍体长穗偃麦草中共鉴定到29个TeHSP70基因，可分为3个亚族，均匀地分布在7条染色体上；其中有26个TeHSP70基因定位于细胞质，且都包含α-螺旋、延伸链、β-折叠和无规则卷曲结构。29个TeHSP70基因在基序上高度保守，但基因结构存在差异，此外在各基因上游均包含多种激素和胁迫响应的顺式作用元件。共线性分析显示，29个TeHSP70基因间存在3对串联重复和片段重复事件；同时与水稻和小麦有着多对同源基因，而与拟南芥同源基因较少。蛋白质互作网络分析显示，有11个TeHSP70蛋白间存在复杂且密切的蛋白质互作网络。表达分析显示，随着盐胁迫的增加（0~300 mmol·L^-1）有7个TeHSP70基因持续上调，有6个TeHSP70基因持续下调，其余16个TeHSP70基因未表达或变化特征不连续；有25个TeHSP70基因在10个不同组织中均有表达，有4个TeHSP70s基因仅在部分组织中表达。以上研究结果为深入解析二倍体长穗偃麦草HSP70功能提供了有效的借鉴和参考。

关键词: 二倍体长穗偃麦草, HSP70, 盐胁迫, 表达模式

CLC Number:

S512.6

Jilin SUN, Jiaqi ZHANG, Fansen MENG, Silong SUN. Genome-wide Identification and Tissue Expression Pattern Analysis of HSP70 Gene Family in Thinopyrum elongatum[J]. Journal of Agricultural Science and Technology, 2025, 27(6): 28-38.

孙吉林, 张家琦, 孟凡森, 孙思龙. 二倍体长穗偃麦草HSP70基因家族鉴定及表达模式分析[J]. 中国农业科技导报, 2025, 27(6): 28-38.

Figures/Tables 10

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基因名称 Gene name	基因ID Gene ID	蛋白质长度Protein length/aa	分子质量Molecular weight/kD	等电点pI	不稳定系数 Instability index	脂肪系数Aliphatic index	总平均亲水性 Grand average of hydropathicity
TeHSP70-01	GWHGABKY001532	887	97.70	5.45	44.50	82.33	-0.458
TeHSP70-02	GWHGABKY001533	835	92.56	5.07	45.59	75.13	-0.513
TeHSP70-03	GWHGABKY001646	740	82.25	5.28	42.34	79.97	-0.380
TeHSP70-04	GWHGABKY001898	734	81.38	5.43	50.39	88.26	-0.333
TeHSP70-05	GWHGABKY003413	686	75.38	5.23	38.34	77.07	-0.503
TeHSP70-06	GWHGABKY011778	683	74.88	5.31	36.86	80.85	-0.427
TeHSP70-07	GWHGABKY012581	680	74.83	5.18	33.92	85.50	-0.381
TeHSP70-08	GWHGABKY012586	690	73.56	5.01	29.29	86.19	-0.270
TeHSP70-09	GWHGABKY016707	665	73.27	5.06	25.54	86.50	-0.479
TeHSP70-10	GWHGABKY017407	683	73.26	5.60	41.92	84.17	-0.325
TeHSP70-11	GWHGABKY022426	684	73.14	5.04	30.07	87.09	-0.310
TeHSP70-12	GWHGABKY022433	665	72.93	5.04	23.51	86.78	-0.371
TeHSP70-13	GWHGABKY022437	678	72.86	5.29	36.04	84.04	-0.310
TeHSP70-14	GWHGABKY022852	678	72.84	5.23	35.63	84.04	-0.303
TeHSP70-15	GWHGABKY024486	684	72.77	5.78	35.28	87.49	-0.268
TeHSP70-16	GWHGABKY024546	677	72.55	5.36	38.85	85.48	-0.292
TeHSP70-17	GWHGABKY024596	657	71.80	5.17	36.09	79.15	-0.446
TeHSP70-18	GWHGABKY025756	653	71.65	5.21	34.54	79.62	-0.458
TeHSP70-19	GWHGABKY026068	667	71.63	5.06	27.89	88.22	-0.296
TeHSP70-20	GWHGABKY027854	640	71.35	5.44	31.47	99.83	-0.088
TeHSP70-21	GWHGABKY030282	660	71.31	5.07	27.51	88.53	-0.319
TeHSP70-22	GWHGABKY032027	648	71.19	5.08	34.79	83.4	-0.394
TeHSP70-23	GWHGABKY034860	648	71.02	5.13	33.73	81.16	-0.415
TeHSP70-24	GWHGABKY035578	651	70.85	7.82	47.67	92.78	-0.118
TeHSP70-25	GWHGABKY035973	620	69.08	6.25	41.66	81.1	-0.402
TeHSP70-26	GWHGABKY042123	617	67.68	8.84	33.40	87.07	-0.173
TeHSP70-27	GWHGABKY042494	582	64.25	5.08	34.38	83.14	-0.417
TeHSP70-28	GWHGABKY043121	583	63.91	5.10	35.91	84.19	-0.390
TeHSP70-29	GWHGABKY043154	544	60.80	7.58	39.85	83.46	-0.388

基因名称 Gene name	基因ID Gene ID	蛋白质长度Protein length/aa	分子质量Molecular weight/kD	等电点pI	不稳定系数 Instability index	脂肪系数Aliphatic index	总平均亲水性 Grand average of hydropathicity
TeHSP70-01	GWHGABKY001532	887	97.70	5.45	44.50	82.33	-0.458
TeHSP70-02	GWHGABKY001533	835	92.56	5.07	45.59	75.13	-0.513
TeHSP70-03	GWHGABKY001646	740	82.25	5.28	42.34	79.97	-0.380
TeHSP70-04	GWHGABKY001898	734	81.38	5.43	50.39	88.26	-0.333
TeHSP70-05	GWHGABKY003413	686	75.38	5.23	38.34	77.07	-0.503
TeHSP70-06	GWHGABKY011778	683	74.88	5.31	36.86	80.85	-0.427
TeHSP70-07	GWHGABKY012581	680	74.83	5.18	33.92	85.50	-0.381
TeHSP70-08	GWHGABKY012586	690	73.56	5.01	29.29	86.19	-0.270
TeHSP70-09	GWHGABKY016707	665	73.27	5.06	25.54	86.50	-0.479
TeHSP70-10	GWHGABKY017407	683	73.26	5.60	41.92	84.17	-0.325
TeHSP70-11	GWHGABKY022426	684	73.14	5.04	30.07	87.09	-0.310
TeHSP70-12	GWHGABKY022433	665	72.93	5.04	23.51	86.78	-0.371
TeHSP70-13	GWHGABKY022437	678	72.86	5.29	36.04	84.04	-0.310
TeHSP70-14	GWHGABKY022852	678	72.84	5.23	35.63	84.04	-0.303
TeHSP70-15	GWHGABKY024486	684	72.77	5.78	35.28	87.49	-0.268
TeHSP70-16	GWHGABKY024546	677	72.55	5.36	38.85	85.48	-0.292
TeHSP70-17	GWHGABKY024596	657	71.80	5.17	36.09	79.15	-0.446
TeHSP70-18	GWHGABKY025756	653	71.65	5.21	34.54	79.62	-0.458
TeHSP70-19	GWHGABKY026068	667	71.63	5.06	27.89	88.22	-0.296
TeHSP70-20	GWHGABKY027854	640	71.35	5.44	31.47	99.83	-0.088
TeHSP70-21	GWHGABKY030282	660	71.31	5.07	27.51	88.53	-0.319
TeHSP70-22	GWHGABKY032027	648	71.19	5.08	34.79	83.4	-0.394
TeHSP70-23	GWHGABKY034860	648	71.02	5.13	33.73	81.16	-0.415
TeHSP70-24	GWHGABKY035578	651	70.85	7.82	47.67	92.78	-0.118
TeHSP70-25	GWHGABKY035973	620	69.08	6.25	41.66	81.1	-0.402
TeHSP70-26	GWHGABKY042123	617	67.68	8.84	33.40	87.07	-0.173
TeHSP70-27	GWHGABKY042494	582	64.25	5.08	34.38	83.14	-0.417
TeHSP70-28	GWHGABKY043121	583	63.91	5.10	35.91	84.19	-0.390
TeHSP70-29	GWHGABKY043154	544	60.80	7.58	39.85	83.46	-0.388

蛋白名称 Protein name	亚细胞定位 Subcelluar localization	α-螺旋 Alpha-helix/%	延伸链 Extended strand/%	β-折叠 Beta-turn/%	无规则卷曲 Random coil/%
TeHSP70-01	周质Periplasmic	43.29	13.19	2.59	40.92
TeHSP70-02	细胞质Cytoplasmic	43.11	14.37	2.75	39.76
TeHSP70-03	细胞质Cytoplasmic	45.41	14.46	2.70	37.43
TeHSP70-04	细胞质Cytoplasmic	48.91	15.12	3.13	32.83
TeHSP70-05	细胞质Cytoplasmic	42.71	17.20	6.27	33.82
TeHSP70-06	细胞质Cytoplasmic	42.90	17.72	6.59	32.80
TeHSP70-07	细胞质Cytoplasmic	40.74	18.53	6.62	34.12
TeHSP70-08	外膜Outermembrane	41.30	19.13	6.23	33.33
TeHSP70-09	细胞质Cytoplasmic	45.11	18.05	6.62	30.23
TeHSP70-10	细胞质Cytoplasmic	43.48	19.62	6.73	30.16
TeHSP70-11	细胞质Cytoplasmic	38.45	20.03	7.75	33.77
TeHSP70-12	细胞质Cytoplasmic	43.46	18.50	6.92	31.13
TeHSP70-13	细胞质Cytoplasmic	38.20	23.16	8.11	30.53
TeHSP70-14	细胞质Cytoplasmic	44.10	19.76	7.82	28.32
TeHSP70-15	外膜Outermembrane	44.88	18.86	6.58	29.68
TeHSP70-16	细胞质Cytoplasmic	44.90	19.65	7.98	27.47
TeHSP70-17	细胞质Cytoplasmic	41.55	18.57	6.39	33.49
TeHSP70-18	细胞质Cytoplasmic	42.57	18.68	6.28	32.47
TeHSP70-19	细胞质Cytoplasmic	44.08	18.59	8.40	28.94
TeHSP70-20	细胞质Cytoplasmic	42.34	22.66	6.56	28.44
TeHSP70-21	细胞质Cytoplasmic	43.03	18.64	8.03	30.30
TeHSP70-22	细胞质Cytoplasmic	41.67	19.44	6.79	32.10
TeHSP70-23	细胞质Cytoplasmic	41.20	16.98	6.48	35.34
TeHSP70-24	细胞质Cytoplasmic	31.18	21.35	6.14	41.32
TeHSP70-25	细胞质Cytoplasmic	43.39	19.03	7.10	30.48
TeHSP70-26	细胞质Cytoplasmic	40.36	19.77	6.97	32.90
TeHSP70-27	细胞质Cytoplasmic	45.70	16.84	6.87	30.58
TeHSP70-28	细胞质Cytoplasmic	44.08	16.12	6.69	33.10
TeHSP70-29	细胞质Cytoplasmic	46.32	18.20	6.99	28.49

蛋白名称 Protein name	亚细胞定位 Subcelluar localization	α-螺旋 Alpha-helix/%	延伸链 Extended strand/%	β-折叠 Beta-turn/%	无规则卷曲 Random coil/%
TeHSP70-01	周质Periplasmic	43.29	13.19	2.59	40.92
TeHSP70-02	细胞质Cytoplasmic	43.11	14.37	2.75	39.76
TeHSP70-03	细胞质Cytoplasmic	45.41	14.46	2.70	37.43
TeHSP70-04	细胞质Cytoplasmic	48.91	15.12	3.13	32.83
TeHSP70-05	细胞质Cytoplasmic	42.71	17.20	6.27	33.82
TeHSP70-06	细胞质Cytoplasmic	42.90	17.72	6.59	32.80
TeHSP70-07	细胞质Cytoplasmic	40.74	18.53	6.62	34.12
TeHSP70-08	外膜Outermembrane	41.30	19.13	6.23	33.33
TeHSP70-09	细胞质Cytoplasmic	45.11	18.05	6.62	30.23
TeHSP70-10	细胞质Cytoplasmic	43.48	19.62	6.73	30.16
TeHSP70-11	细胞质Cytoplasmic	38.45	20.03	7.75	33.77
TeHSP70-12	细胞质Cytoplasmic	43.46	18.50	6.92	31.13
TeHSP70-13	细胞质Cytoplasmic	38.20	23.16	8.11	30.53
TeHSP70-14	细胞质Cytoplasmic	44.10	19.76	7.82	28.32
TeHSP70-15	外膜Outermembrane	44.88	18.86	6.58	29.68
TeHSP70-16	细胞质Cytoplasmic	44.90	19.65	7.98	27.47
TeHSP70-17	细胞质Cytoplasmic	41.55	18.57	6.39	33.49
TeHSP70-18	细胞质Cytoplasmic	42.57	18.68	6.28	32.47
TeHSP70-19	细胞质Cytoplasmic	44.08	18.59	8.40	28.94
TeHSP70-20	细胞质Cytoplasmic	42.34	22.66	6.56	28.44
TeHSP70-21	细胞质Cytoplasmic	43.03	18.64	8.03	30.30
TeHSP70-22	细胞质Cytoplasmic	41.67	19.44	6.79	32.10
TeHSP70-23	细胞质Cytoplasmic	41.20	16.98	6.48	35.34
TeHSP70-24	细胞质Cytoplasmic	31.18	21.35	6.14	41.32
TeHSP70-25	细胞质Cytoplasmic	43.39	19.03	7.10	30.48
TeHSP70-26	细胞质Cytoplasmic	40.36	19.77	6.97	32.90
TeHSP70-27	细胞质Cytoplasmic	45.70	16.84	6.87	30.58
TeHSP70-28	细胞质Cytoplasmic	44.08	16.12	6.69	33.10
TeHSP70-29	细胞质Cytoplasmic	46.32	18.20	6.99	28.49

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