Abstract:

As a plant-specific transcription factor， growth regulating factor （GRF） plays an important role in plant growth and development. To identify the members of the rice GRF gene family， protein physicochemical properties， sequence comparison， secondary and tertiary structure prediction， subcellular localisation， gene structure， cis-acting elements， chromosomal localisation， evolutionary tree construction and covariance were analyzed using the bioinformatics methods. The results showed that 12 GRF genes were identified. The length of rice GRF proteins was 211~456 aa， the molecular weight was 22.3~49.3 kD， the isoelectric point was 4.78~9.85， and the subcellular localisation was mainly located in the nucleus. The rice GRF genes contained 2~5 exons， and the promoter elements were mainly related to hormone regulation， stress response and light response. 12 GRF genes were unevenly distributed on chromosomes 2， 3， 4， 6， 7， 11 and 12. GRF genes in rice were closely related to maize， and had low homology with tomato and Arabidopsis. There were no tandem duplication events in the rice GRF genes， and large segmental duplications were the main expansion modes. Above results provided a theoretical basis for the in-depth study of rice GRF gene function.

Key words: rice, growth regulating factor, GRF gene family, bioinformatics

摘要：

生长调节因子（growth regulating factor，GRF）作为一种植物特有的转录因子，在植物生长发育过程中发挥重要作用。为鉴定水稻GRF基因家族成员，通过生物信息学方法对其蛋白的理化性质、二级结构、三级结构、亚细胞定位、基因结构、顺式作用元件、染色体定位、进化关系和共线性进行分析。结果表明，共鉴定出12个水稻GRF基因，其蛋白长度为211~456 aa，分子量为22.3~49.3 kD，等电点为4.78~9.85，亚细胞定位主要定位于细胞核；水稻GRF基因含有2~5个外显子，启动子顺式作用元件主要与激素调控、胁迫响应、光响应相关；12个GRF基因不均匀地分布在2、3、4、6、7、11、12号染色体上；水稻GRF基因与玉米亲缘关系较近，与番茄、拟南芥同源性较低；水稻GRF基因不存在串联重复事件，大片段重复是其主要扩张方式。以上研究结果为深入研究水稻GRF基因功能提供了理论依据。

关键词: 水稻, 生长调节因子, GRF基因家族, 生物信息学