桃自根砧对NaCl生理生化响应及耐盐阈值分析

doi:10.13304/j.nykjdb.2023.0271

摘要/Abstract

摘要：

为研究NaCl胁迫下桃自根砧生长及生理生化指标变化，确定桃自根砧耐盐阈值，选择苗龄为1年的樱桃李（Prunus cerasifera Ehrhart）1号（李1）、樱桃李3号（李3）、樱桃李5号（李5）、RA （Prunus cerasifera Ehrhart）和GF677（Prunus dulcis Mill.×Prunus persica）桃砧木扦插苗为试材进行NaCl处理（质量体积分数0.2%、0.4%和0.6%NaCl）试验。结果表明，随着基质中NaCl含量的增加，5种桃砧木扦插苗的超氧化物歧化酶（superoxide dismutase，SOD）活性和丙二醛（malondialdehyde，MDA）含量均呈上升趋势；过氧化物酶（peroxidase，POD）活性在李1、李3中先升后降，在李5、GF677中则相反，在RA中呈上升趋势；过氧化氢酶（catalase，CAT）活性在李1、李3、RA中呈上升趋势，在李5中呈下降趋势，在GF677则先升后降。脯氨酸（proline，Pro）含量在李1和RA中呈上升趋势，在李3和李5中先降后升，在GF677中先升后降。可溶性糖含量在李1、RA中先升后降，在李3和李5中呈上升趋势，在GF677中先降后升。相关性分析表明，Na⁺水平与基质中NaCl含量成正相关，而K⁺水平则与其呈负相关关系。李1的Na⁺/K⁺最大，GF677最小。在NaCl胁迫下，5种自根砧叶绿素合成明显受到抑制，荧光动力学参数以及光能吸收、捕获和传递参数均降低，快速叶绿素荧光诱导曲线（OJIP）发生变化，其中J、I、P 相降低，且ΔK小于0。以净光合速率为指标，李1、李3、李5、RA和GF677的耐盐性阈值分别为0.37%、0.43%、0.38%、0.46%和0.49% NaCl。综合隶属函数、耐盐系数及耐盐阈值，砧木耐盐性从高到低表现为GF677>RA>李3>李5>李1。综上所述，5种桃自根砧主要通过活性氧清除和渗透调节机制来适应盐胁迫，耐盐性高的砧木能较好地稳定光合反应中心，缓解盐胁迫对其光合系统的伤害。以上结果为揭示耐盐机理及桃耐盐砧木品种的筛选提供理论支撑。

关键词: 桃自根砧, 盐胁迫, 荧光特性, 生理生化

Abstract:

In order to explore the growth and physiological and biochemical responses of the peach self-rooted rootstock under NaCl stress and determine the salinity tolerance threshold， the cutting seedlings of one-year-old 5 rootstocks were treated with different NaCl contents （0.2%， 0.4% and 0.6% NaCl）. The results showed that， with the increase of NaCl content， the activity of superoxide dismutase （SOD） and content of malondialdehyde （MDA） increased. With the increase of NaCl content， the activity of peroxidase （POD） increased first and then decreased in Li 1 and Li 3， while decreased first and then increased in Li 5 and GF677， and showed increasing trend in RA. With the increase of NaCl content， the activity of catalase （CAT） showed increasing trend in Li 1， Li 3 and RA， showed decreasing trend in Li 5， and increased first and then decreased in GF677. The content of Pro increased in Li and RA， decreased first and then increased in Li 3 and Li 5， and increased first and then decreased in GF677. The content of soluble sugar increased in Li 3 and Li 5， increased first and then decreased in Li 1 and RA， and decreased first and then increased in GF677. The correlation analysis showed that Na⁺ content was positively related to NaCl content in media， while K⁺ content was negative correlation with NaCl content. The Na⁺/K⁺ was the highest in Li 1， while was the lowest in GF677. Under NaCl stress， the chlorophyll synthesis of 5 peach rootstocks was significantly inhibited. Fluorescence kinetic parameters and light energy absorption， capture and transfer parameters were reduced， and the curve of OJIP changed with the decrease of J， I， P phases and ΔK<0. Considering net photosynthetic rate （P_n） as a tolerance index， the salt tolerance thresholds of Li 1， Li 3， Li 5， RA and GF677 were 0.37%， 0.43%， 0.38%， 0.46% and 0.49% NaCl， respectively. According to comprehensive membership function， salt tolerance coefficient index and salt tolerance thresholds resistance， the salt tolerance showed GF677>RA>Li 3> Li 5>Li 1.In summary， self rooting stocks of peach adapted to salt stress mainly through active oxygen scavenging and osmotic adjustment mechanisms， rootstocks with high salt tolerance could better stabilize the photosynthetic reaction center and alleviate the damage of salt stress to their photosynthetic system， which provided theoretical support for revealing the mechanism of salt tolerance and screening the varieties of peach rootstock with high salt tolerant.

Key words: peach rootstock, salt stress, fluorescence characteristic, physiological biochemistry

中图分类号:

S662.1

张帆, 王鸿, 张雪冰, 陈建军. 桃自根砧对NaCl生理生化响应及耐盐阈值分析[J]. 中国农业科技导报, 2023, 25(11): 70-79.

Fan ZHANG, Hong WANG, Xuebing ZHANG, Jianjun CHEN. Physiological and Biochemical Responses of Peach Self-rooted Rootstock to NaCl and Analysis of Salt Tolerance Threshold[J]. Journal of Agricultural Science and Technology, 2023, 25(11): 70-79.

图/表 10

参考文献 17

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品种Variety	NaCl含量 NaCl content/%	F_v/F_m	PI_ABS	PI_total	φ_Eo	φ_Ro
李1 Li 1	0.2	0.829±0.011 abc	1.959±0.331 b	0.798±0.20 cdef	1.221±0.168 ab	0.401±0.035 efg
	0.4	0.671±0.032 e	0.183±0.002 c	0.195±0.001 ef	0.898±0.068 bc	0.459±0.040 ef
	0.6	0.632±0.011 f	0.008±0.001 c	0.011±0.000 f	0.284±0.005 d	0.464±0.035 e
李3 Li 3	0.2	0.832±0.004 abc	3.425±0.476 ab	1.765±0.376 abc	0.975±0.052 bc	0.448±0.041 efg
	0.4	0.831±0.005 abc	3.130±0.010 ab	1.704±0.120 abcd	1.196±0.041 b	0.346±0.002 g
	0.6	0.825±0.015 abc	2.741±1.156 ab	1.087±0.007 cde	0.632±0.020 c	0.503±0.035 de
李5 Li 5	0.2	0.810±0.040 bc	1.995±0.366 b	1.582±0.657 bcd	0.883±0.078 bc	0.351±0.023 fg
	0.4	0.805±0.003 c	2.196±2.128 b	0.957±0.999 cdef	1.149±0.125 b	0.570±0.113 cd
	0.6	0.723±0.006 d	2.694±0.621 ab	0.701±0.139 def	0.927±0.575 bc	0.406±0.047 efg
RA	0.2	0.838±0.001 ab	3.417±0.043 ab	1.532±0.154 bcd	1.222±0.196 ab	0.668±0.078 c
	0.4	0.834±0.008 ab	3.241±0.69 ab	1.508±0.460 bcd	1.096±0.052 b	0.624±0.129 c
	0.6	0.832±0.006 ab	3.125±0.84 ab	1.471±0.510 bcd	0.897±0.268 bc	0.622±0.023 c
GF677	0.2	0.845±0.002 a	4.364±1.06 a	2.611±1.200 a	1.539±0.015 a	1.557±0.001 a
	0.4	0.843±0.007 a	4.018±0.498 a	2.125±0.499 ab	1.159±0.010 b	1.075±0.007 b
	0.6	0.841±0.003 a	4.023±0.414 a	1.784±0.376 abc	1.111±0.129 b	0.616±0.083 c

品种Variety	NaCl含量 NaCl content/%	F_v/F_m	PI_ABS	PI_total	φ_Eo	φ_Ro
李1 Li 1	0.2	0.829±0.011 abc	1.959±0.331 b	0.798±0.20 cdef	1.221±0.168 ab	0.401±0.035 efg
	0.4	0.671±0.032 e	0.183±0.002 c	0.195±0.001 ef	0.898±0.068 bc	0.459±0.040 ef
	0.6	0.632±0.011 f	0.008±0.001 c	0.011±0.000 f	0.284±0.005 d	0.464±0.035 e
李3 Li 3	0.2	0.832±0.004 abc	3.425±0.476 ab	1.765±0.376 abc	0.975±0.052 bc	0.448±0.041 efg
	0.4	0.831±0.005 abc	3.130±0.010 ab	1.704±0.120 abcd	1.196±0.041 b	0.346±0.002 g
	0.6	0.825±0.015 abc	2.741±1.156 ab	1.087±0.007 cde	0.632±0.020 c	0.503±0.035 de
李5 Li 5	0.2	0.810±0.040 bc	1.995±0.366 b	1.582±0.657 bcd	0.883±0.078 bc	0.351±0.023 fg
	0.4	0.805±0.003 c	2.196±2.128 b	0.957±0.999 cdef	1.149±0.125 b	0.570±0.113 cd
	0.6	0.723±0.006 d	2.694±0.621 ab	0.701±0.139 def	0.927±0.575 bc	0.406±0.047 efg
RA	0.2	0.838±0.001 ab	3.417±0.043 ab	1.532±0.154 bcd	1.222±0.196 ab	0.668±0.078 c
	0.4	0.834±0.008 ab	3.241±0.69 ab	1.508±0.460 bcd	1.096±0.052 b	0.624±0.129 c
	0.6	0.832±0.006 ab	3.125±0.84 ab	1.471±0.510 bcd	0.897±0.268 bc	0.622±0.023 c
GF677	0.2	0.845±0.002 a	4.364±1.06 a	2.611±1.200 a	1.539±0.015 a	1.557±0.001 a
	0.4	0.843±0.007 a	4.018±0.498 a	2.125±0.499 ab	1.159±0.010 b	1.075±0.007 b
	0.6	0.841±0.003 a	4.023±0.414 a	1.784±0.376 abc	1.111±0.129 b	0.616±0.083 c

品种Variety	NaCl含量 NaCl content/%	ABC/CS_m	TR_o/CS_m	ET_O/CS_m	RC/CS_m
李1 Li 1	0.2	1 969.00±26.664 ab	1 479.00±158.072 ab	746.00±94.000 ab	275.33±10.682 abc
	0.4	1 725.33±5.131 b	1 433.33±1.527 ab	696.67±185.403 ab	201.33±74.865 cde
	0.6	1 705.33±5.231 b	1 410.67±431.430 ab	678.67±397.945 ab	155.33±1.763 e
李3 Li 3	0.2	1 846.67±89.001 ab	1 674.906±128 ab	882.67±64.663 ab	276.50±6.639 abc
	0.4	1 961.00±1.000 ab	1 583.33±0.577 ab	841.00±61.991 ab	247.33±0.333 abcd
	0.6	1 846.50±100.500 ab	1 524.50±110.500 ab	831.00±73.000 ab	215.00±23.094 bcde
李5 Li 5	0.2	2 000.00±85.422 ab	1 654.67±1.527 ab	772. 00±59.000 ab	209.00±38.214 bcde
	0.4	1 816.33±51.286 ab	1 510.00±43.863 ab	707.00±35.552 ab	183.33±4.484 de
	0.6	1 778.33±106.800 ab	1 398.67±216.705 abc	591.33±366.444 b	148.00±0.577 e
RA	0.2	1 903.00±1.000 ab	1 677.00±87.298 ab	890.67±7.094 ab	290.67±27.088 abc
	0.4	1 755.67±188.797 ab	1 658.33±34.789 ab	823.00±2.000 ab	280.33±20.177 abc
	0.6	1 896.00±140.000 ab	1 574.00±136.000 ab	791. 00±2.000 ab	275.33±35.025 bcd
GF677	0.2	2 028.67±6.806 a	1 693.33±7.2342 a	961.00±1.000 a	329.33±14.858 a
	0.4	1 972.00±48.692 ab	1 562.50±77.500 ab	920.33±94.659 a	321.33±28.309 a
	0.6	1 862.50±78.500 ab	1 658.33±39.106 ab	866. 33±4.932 ab	296.50±17.031 ab

品种Variety	NaCl含量 NaCl content/%	ABC/CS_m	TR_o/CS_m	ET_O/CS_m	RC/CS_m
李1 Li 1	0.2	1 969.00±26.664 ab	1 479.00±158.072 ab	746.00±94.000 ab	275.33±10.682 abc
	0.4	1 725.33±5.131 b	1 433.33±1.527 ab	696.67±185.403 ab	201.33±74.865 cde
	0.6	1 705.33±5.231 b	1 410.67±431.430 ab	678.67±397.945 ab	155.33±1.763 e
李3 Li 3	0.2	1 846.67±89.001 ab	1 674.906±128 ab	882.67±64.663 ab	276.50±6.639 abc
	0.4	1 961.00±1.000 ab	1 583.33±0.577 ab	841.00±61.991 ab	247.33±0.333 abcd
	0.6	1 846.50±100.500 ab	1 524.50±110.500 ab	831.00±73.000 ab	215.00±23.094 bcde
李5 Li 5	0.2	2 000.00±85.422 ab	1 654.67±1.527 ab	772. 00±59.000 ab	209.00±38.214 bcde
	0.4	1 816.33±51.286 ab	1 510.00±43.863 ab	707.00±35.552 ab	183.33±4.484 de
	0.6	1 778.33±106.800 ab	1 398.67±216.705 abc	591.33±366.444 b	148.00±0.577 e
RA	0.2	1 903.00±1.000 ab	1 677.00±87.298 ab	890.67±7.094 ab	290.67±27.088 abc
	0.4	1 755.67±188.797 ab	1 658.33±34.789 ab	823.00±2.000 ab	280.33±20.177 abc
	0.6	1 896.00±140.000 ab	1 574.00±136.000 ab	791. 00±2.000 ab	275.33±35.025 bcd
GF677	0.2	2 028.67±6.806 a	1 693.33±7.2342 a	961.00±1.000 a	329.33±14.858 a
	0.4	1 972.00±48.692 ab	1 562.50±77.500 ab	920.33±94.659 a	321.33±28.309 a
	0.6	1 862.50±78.500 ab	1 658.33±39.106 ab	866. 33±4.932 ab	296.50±17.031 ab

品种Variety	叶绿素Chlorophyll	丙二醛MDA	脯氨酸Pro	可溶性糖Soluble sugar	超氧化物歧化酶SOD	过氧化物酶POD	过氧化氢酶CAT	Na⁺	K⁺	均值Mean	排序Ranking
李1 Li 1	0.84	0.57	0.54	0.38	0.17	0.17	0.25	0.50	0.32	0.42	5
李3 Li 3	0.91	0.39	0.61	0.34	0.44	0.44	0.50	0.68	0.27	0.51	3
李5 Li 5	0.67	0.50	0.24	0.44	0.45	0.45	0.67	0.58	0.42	0.49	4
RA	0.12	0.90	0.59	0.72	0.54	0.54	0.65	0.69	0.5	0.58	2
GF677	0.91	0.51	0.62	0.41	0.73	0.73	0.92	0.33	0.66	0.66	1