Proteinome and Metabolome Combined to Analyze the Response Mechanism of Oat to Saline-alkali Stress

doi:10.13304/j.nykjdb.2024.0118

Abstract

Abstract:

Salt-alkali stress is one of the main abiotic stresses limits plant growth. Planting oats in saline-alkali land allows the oat stems to absorb salt from the soil and store it in oat straw to repair the saline-alkali land. In order to clarify the effects of salt-alkali stress on oat proteome and metabolome， four-week-old oats were treated with 6， 12， 24 and 48 h salt-alkali stress， and the physiological and biochemical properties of oats after salt-alkali stress were measured， and its proteome and metabolome were analyzed. The results showed that， with the prolongation of salt-alkali stress time， malondialdehyde content of oat increased overall， the proline content and the activities of superoxide dismutase， peroxidase， catalase generally showed an upward trend. Proteomic and metabolomic sequencing identified a total of 7 603 differential proteins， including 3 217 up-regulated， 4 386 down-regulated and 855 differential metabolites. They were mainly involved in pathways such as sugar metabolism， amino acid metabolism， photosynthesis and phenylpropanoid biosynthesis. Among them， lycopeneβ-cyclase， cyclooxygenase and zeaxanthin were key enzymes related to photosynthesis. In summary， salt-alkali stress enhanced the accumulation of metabolites such as sugars， nucleosides and amino acids. Ribosomes， phenylpropanoid biosynthesis， glyoxylic acid and dicarboxylic acid metabolism， and carbon fixation in photosynthetic organisms of leaves were the main pathways responsed salt-alkali stress. Above resltus provided effective informations for the restoration of saline-alkali soil and the reconstruction of ecosystems， and laid a theoretical foundation for the genetic improvement and cultivation of oat stress resistance.

Key words: oat, proteomics, metabolomics, joint analysis, saline-alkali stress

摘要：

盐碱胁迫是限制植物生长的主要非生物胁迫之一。在盐碱地种植燕麦，可以使燕麦茎吸收土壤中的盐，并储存在燕麦秸秆中，以修复盐碱地。为明确盐碱胁迫对燕麦蛋白组及代谢组的影响，对4周龄燕麦进行6、12、24、48 h盐碱胁迫，测定不同胁迫后燕麦的生理生化指标，并对其蛋白组和代谢组进行分析。结果表明，随着盐碱胁迫时间的延长，燕麦的丙二醛含量总体上升，脯氨酸含量及超氧化物歧化酶、过氧化物酶、过氧化氢酶活性总体呈上升趋势。蛋白组和代谢组分析共鉴定到7 603个差异蛋白，其中上调3 217个、下调4 386个；差异代谢物855个。它们主要参与糖代谢、氨基酸代谢、光合作用和苯丙烷类生物合成等途径，包括番茄红素β-环化酶、环氧化酶和玉米黄质等与光合作用相关的关键酶。综上，盐碱胁迫增强了燕麦糖类、核苷酸、氨基酸等代谢物的积累，叶片中核糖体、苯丙烷类生物合成、乙醛酸和二羧酸代谢、光合生物中的碳固定是燕麦响应盐碱胁迫的主要途径。以上研究结果为盐碱土壤的恢复以及生态系统的重建提供了有效信息，为燕麦抗逆遗传改良和栽培奠定了理论基础。

关键词: 燕麦, 蛋白质组, 代谢组, 联合分析, 盐碱胁迫

CLC Number:

S512.6

Lei LING, Huixin JIANG, Mingjing LI, Yajie YIN, Naiyu CHEN, Xiaoju ZHAO. Proteinome and Metabolome Combined to Analyze the Response Mechanism of Oat to Saline-alkali Stress[J]. Journal of Agricultural Science and Technology, 2025, 27(5): 61-71.

凌磊, 蒋慧欣, 李铭婧, 殷亚杰, 陈乃钰, 赵晓菊. 盐碱胁迫下燕麦蛋白组及代谢组联合分析[J]. 中国农业科技导报, 2025, 27(5): 61-71.

Figures/Tables 6

Fig. 1 Biochemical indicators after salinity stress

Fig. 2 Oat proteome sequencing results after saline stressA： Heat map of sample correlation，_1~_3 indicate 3 repetitions； B： PCA analysis； C： Venn diagram of differential proteins； D： Statistics of differential proteins

Fig. 3 Differential protein KEGG enrichment analysis

Fig. 4 Metabolome analysis of oat under different saline stress treatmentsA： Heat map of correlation，_1~_6 indicate 6 repetitions； B： PCA analysis； C： Venn diagram of differential metabolites； D： KEGG enrichment

Fig. 5 Loplots of differential protein and differential metabolite OPLSNote：Pq［1］ indicates covariance between principal component and metabolite （protein）；pq［2］ indicates corretation coefficient between principal component and metabolite （protein）.

Fig. 6 Protein and metabolism are jointly enriched in the top ten pathways

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