Physiochemical Properties and Microbial Community Structure in Rhizosphere Soil of Dracocephalum tanguticum

doi:10.13304/j.nykjdb.2022.0741

Abstract

Abstract:

In order to study the physiochemical properties and microbial community structure in rhizosphere soil of Dracocephalum tanguticum， the microbial communities in rhizosphere soil from Gongbujiangda （GB）， Kanuo （KR） and Luolong （LL） in Tibet were as materials. The microbial community in rhizosphere soil of D. tanguticum was analyzed by illumine high-throughput sequencing， and the physiochemical properties were determined and their correlation with core microbial communities were studied. The results showed that there were significant differences among different regions in the physiochemical properties. A total of 3 900 bacterial OTUs and 1 990 fungal OTUs were obtained in rhizosphere soil of D. tanguticum from 3 regions. The microbial diversity index in the rhizosphere soil of D. tanguticum grown in different regions was significant difference. Actinobacteria was the dominant bacterial phyla in all samples of D. tanguticum. Basidiomycota was the dominant fungal phyla in GB sample， Ascomycota was the dominant fungal phyla in KR and LL samples. The dominant genus was difference among different samples. Principal component analysis showed that the compositions of bacterial and fungal community were different among different samples. The analysis of core microbial communities showed that there were 257 core bacterial genera and 102 fungal genera. The correlation analysis showed that the changes of microbial community in rhizosphere soil were correlated with the physiochemical properties， and the contents of total potassium， available potassium， total phosphorus， total nitrogen and available nitrogen were the key determinants affecting the composition of microbial community in rhizosphere soil of D. tanguticum. Overall， the microbial community structure in rhizosphere soil of D. tanguticum grown in the different regions were significant differences， and the microbial community structure of rhizosphere soil was strongly linked to the physiochemical properties of rhizosphere soil. Above results provided theoretical bases for artificial cultivation and screening beneficial microorganisms of D. tanguticum.

Key words: Dracocephalum tanguticum, soil physiochemical properties, high-throughput sequencing, microbial community, core microbial, correlation analysis

摘要：

为研究甘青青兰根际土壤理化性质与根际土壤细菌和真菌群落结构组成特征，以西藏自治区工布江达县（GB）、卡诺区（KR）和洛隆县（LL）的甘青青兰根际土壤微生物为研究对象，采用Illumina MiSeq高通量测序技术对根际土壤细菌和真菌群落结构组成进行分析，同时测定根际土壤的理化性质，并与根际土壤核心微生物菌群进行相关性分析。结果表明，不同地区甘青青兰根际土壤理化性质差异显著。甘青青兰根际土壤共获得3 900个细菌OTUs和1 990个真菌OTUs，不同地区间土壤微生物多样性存在明显差异。在门水平，GB、KR和LL样品的优势细菌门均为放线菌门（Actinobacteria），GB样品的优势真菌门是担子菌门（Basidiomycota），KR和LL样品的优势真菌门是子囊菌门（Ascomycota）；在属水平，不同地区甘青青兰根际土壤优势微生物菌群存在明显差异。主成分分析显示，不同地区甘青青兰根际土壤细菌和真菌群落结构组成差异较大。核心微生物菌群分析表明，甘青青兰根际土壤核心细菌菌群有257个属；核心真菌菌群有102个属。相关性分析表明，核心微生物菌群的改变与根际土壤理化因子间存在不同程度的相关性，且土壤全钾、全磷、全氮、速效钾和速效氮含量是影响甘青青兰根际土壤微生物群落结构组成的重要因子。综上所述，不同地区甘青青兰根际土壤微生物群落结构组成存在显著差异，且根际土壤微生物群落结构的变化与土壤理化因子密切相关，为甘青青兰人工种植及筛选有益微生物菌群提供了理论依据。

关键词: 甘青青兰, 土壤理化性质, 高通量测序, 微生物群落, 核心微生物, 相关性分析

CLC Number:

S154.3

Erhao ZHANG, Panpan LIU, Ping HE, Yue JIAN, Yuting XU, Chengxin CHEN, Yazhou LU, Xiaozhong LAN, Sangmu SUOLANG. Physiochemical Properties and Microbial Community Structure in Rhizosphere Soil of Dracocephalum tanguticum[J]. Journal of Agricultural Science and Technology, 2024, 26(3): 201-213.

张二豪, 刘盼盼, 何萍, 简阅, 徐雨婷, 陈诚欣, 禄亚洲, 兰小中, 索朗桑姆. 甘青青兰根际土壤理化性质及微生物群落结构特征分析[J]. 中国农业科技导报, 2024, 26(3): 201-213.

Figures/Tables 13

Table 1 Information of sampling site

样品采集点 Sampling site

经度 Longitude

纬度

Latitude

Fig. 1 Physical and chemical properties of rhizosphere soilNote： Different lowercase letters indicate significant differences between different samples at P<0.05 level.

Table 2 OTUs and related sequence indexes in rhizosphere soil of D. tanguticum

样品 Sample	有效序列数Effective sequence number		平均长度Average length/bp		测序覆盖度Sequencing coverage/%		OTUs数量Number of OTUs
样品 Sample	细菌 Bacteria	真菌Fungi	细菌 Bacteria	真菌Fungi	细菌 Bacteria	真菌Fungi	细菌 Bacteria	真菌Fungi
GB	15 316	42 048	376	284	97.78	99.83	1 149	489
KR	16 714	51 312	376	243	97.11	99.67	1 473	883
LL	16 733	50 384	376	252	97.46	99.85	1 278	618

Fig. 2 Venn diagrams of microbial OTUs detected in the rhizosphere soil of D. tanguticum.

Fig. 3 α diversity indexes of microbial community in rhizosphere soil of D. tanguticum.Note： *， ** and *** represent significant differences between different samples at P<0.05， P<0.01 and P<0.001 levels， respectively.

Table 3 Total numbers of bacterial and fungal taxa detected in the rhizosphere soil of D. tanguticum.

样品Sample	门Phylum		纲Class		目Order		科Family		属Genus
样品Sample	细菌 Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi	细菌Bacteria	真菌Fungi
GB	26	10	62	28	133	66	197	132	360	190
KR	26	9	68	31	159	83	237	163	415	276
LL	23	8	55	29	125	62	193	137	332	225

Fig. 4 Composition of microbial community in the rhizosphere soil of D. tanguticum at phylum level

Fig. 5 Composition of microbial community in the rhizosphere soil of D. tanguticum at genus level

Fig. 6 Cluster analysis of microbial community in the rhizosphere soil of D. tanguticum

Fig. 7 Principal component analysis of microbial community in the rhizosphere soil of D. tanguticum

Fig. 8 Venn diagram of key microbial in the rhizosphere soil of D. tanguticum. at genus level

Fig. 9 Correlation between physical and chemical properties and core microbiota in the rhizosphere soil.Note： *， ** and *** represent significant correlations at P<0.05， P<0.01 and P<0.001 levels， respectively.

Fig. 10 Correlation between physical， chemical properties and microbial diversity index in rhizosphere soilNote： *， ** and *** represent significant correlations at P<0.05， P<0.01 and P<0.001 levels， respectively.

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