Abstract:

Bakanae disease， a common and highly harmful fungal disease of rice， seriously impacts rice yield. To clarify the up-regulated differential proteins of high-resistance varieties in response to the bakanae pathogen， the TMT （tandem mass tag） quantitative proteomics technology was employed to compare the proteomes before and after infection with the bakanae pathogen， and the significantly differentially expressed proteins with P≤0.05 and a fold change （FC）>1.2 or <0.83 were screened. The results showed that 124 up-regulated and 75 down-regulated differentially expressed proteins were screened. The 124 proteins with up-regulated expression were subjected to gene ontology （GO） enrichment analysis， and got 88 enrichment items， among which 4 items were significantly enriched， including 2 biological processes （response to stress and response to oxidative stress）， 2 molecular functions （peroxidase activity and heme binding）， which should be beneficial to remove peroxides in rice bud tissue and had a protective effect on cells. A total of 45 pathways were obtained by （kyoto encyclopedia of genes and genomes KEGG） enrichment analysis， and 3 pathways were significantly enriched for upregulated differential proteins including phenylpropanoid biosynthesis， the biosynthesis of secondary metabolites and phenylalanine metabolism， respectively， which should be beneficial toimprove disease resistance of rice. There were 3 differential proteins （Os01t0327400-01， Os12t0112000-01， Os01t0963000-04） were common among 4 items of GO significant enrichment and 3 pathways of KEGG significant enrichment， which could be the key proteins in the bakanae disease resistance. Above results provided scientific theoretical basis for the research of disease resistance.

Key words: rice, bakanae disease, Gibberella fujikuroi species complex, proteome, disease resistance

摘要：

恶苗病是危害性强的水稻常见真菌性病害，严重影响水稻产量。为明确高抗品种响应恶苗病菌的上调差异蛋白质，采用 TMT （tandem mass tag）定量蛋白质组学技术比较感染恶苗病菌前后的蛋白质组，筛选P≤0.05且差异倍数（fold change，FC）>1.2或<0.83的显著差异表达蛋白质。结果表明，共筛选出124个上调差异表达蛋白质和75个下调差异表达蛋白质。将上调表达的124个蛋白质进行GO（gene ontology）富集分析，得到88个富集条目，其中显著性富集条目4个，包括生物过程2个、分子功能2个，分别为对应激的反应、对氧化应激的反应及过氧化物酶活性、血红素结合，有利于清除水稻芽组织内过氧化物，对细胞起保护作用。KEGG（kyoto encyclopedia of genes and genomes）富集分析共得到45条通路，其中上调差异蛋白质显著富集通路3条，分别为苯丙素生物合成、次生代谢物的生物合成和苯丙氨酸代谢，有利于提高水稻抗病性。在显著性GO富集的4个条目和KEGG显著性富集的3条通路中，共有的上调差异蛋白质有3个（Os01t0327400-01、Os12t0112000-01、Os01t0963000-04），推测这3个上调差异蛋白质是抗恶苗病的关键蛋白。以上研究结果为抗病研究工作提供科学的基础理论依据。

关键词: 水稻, 恶苗病, 藤仓赤霉复合种, 蛋白质组, 抗病性