Effects of Shading Cultivation on Leaf Uniformity of Cigar-wrapper Tobacco （Nicotiana tabacum）

doi:10.13304/j.nykjdb.2022.0706

Abstract

Abstract:

In order to explore the influence of shading cultivation on the leaf uniformity of cigar-wrapper tobacco， a cultivar‘Chuxue 26’ （CX-26） was used as material to conduct different shading treatments in the field， and the leaf thickness， leaf flatness， leaf density， main and branch vein thickness， proportion of leaf vein to leaf dry weight， and SPAD value of the upper and middle leaves at maturity were analyzed. The results showed that the leaf thickness， leaf density and SPAD value of upper leaves decreased gradually with the increase of shading degree. When the light transmittance was 70%， the leaf thickness and leaf density decreased by 35.5% and 33.3%， respectively，compared with the treatment without shading， while they increased gradually in middle leaves. Shade cultivation significantly increased the leaf flatness of upper leaves， but decreased the leaf flatness of middle leaves. When the light transmittance was 70%， the leaf flatness of the upper leaves reached the maximum， which was 0.98. In addition， shading cultivation increased the proportion of the main branches in the upper leaves to the leaf dry matter， and the influence on the proportion of the main branches in the middle leaves to the leaf dry matter showed a downward trend with the increase of shading degree， but no significant difference was observed. Moreover， shading cultivation had no effect on the thickness of the main branches of the middle leaves， but it made the main branches of the upper leaves thinner. Above results provided a theoretical basis for shading cultivation techniques of domestic cigar tobacco.

Key words: cigar tobacco, shading cultivation, light transmittance, leaf uniformity

摘要：

为探究遮阴栽培对雪茄茄衣品种叶面均匀性的影响，以雪茄茄衣品种‘楚雪26号’（CX-26）为研究对象，在大田试验条件下进行不同程度的遮阴处理，分析其成熟期上部叶和中部叶的叶面厚度、叶面平展度、叶片密度、主支脉粗细、主支脉占叶片干物质量比例以及SPAD值等的差异。结果表明，随着遮阴程度的增加，上部叶的叶面厚度、叶片密度以及叶片SPAD值均逐渐下降；当透光率为70%时，叶面厚度、叶片密度与不遮阴处理相比分别下降35.5%和33.3%，而中部叶中各性状则逐渐上升。遮阴栽培显著增加上部叶的叶面平展度，但降低了中部叶的叶面平展度；在透光率为70%时，上部叶的叶面平展度达到最大，为0.98；遮阴栽培增加上部叶主支脉占叶片干物质量比例，对中部叶主支脉占叶片干物质量比例的影响随遮阴程度的增加呈下降趋势，但差异不显著。遮阴栽培对中部叶主支脉的粗细程度无影响，但使上部叶的主支脉变细。以上研究结果为国产雪茄茄衣烟叶遮阴栽培技术提供理论基础。

关键词: 雪茄烟, 遮阴栽培, 透光率, 叶面均匀性

CLC Number:

S572

Hao GUO, Ronglei TAN, Jinpeng YANG, Jun YU, Wenchang HUANG, Jiuhong YANG, Baoming QIAO, Ruiwei YANG, Fangsen XU, Chunlei YANG, Guangda DING. Effects of Shading Cultivation on Leaf Uniformity of Cigar-wrapper Tobacco （Nicotiana tabacum）[J]. Journal of Agricultural Science and Technology, 2024, 26(2): 216-225.

郭昊, 谭荣雷, 杨锦鹏, 余君, 黄文昌, 杨久红, 乔保明, 杨瑞玮, 徐芳森, 杨春雷, 丁广大. 遮阴栽培对雪茄茄衣烟叶叶面均匀性的影响[J]. 中国农业科技导报, 2024, 26(2): 216-225.

Figures/Tables 8

Fig. 1 Schematic diagram of sampling parts of cigar tobacco leavesNote： ①~⑧ represent 8 circular sampling points used to measure the thickness and density of the leaf， and 3 dotted lines represent 3 measuring lines for analyzing the flatness of the leaf.

Fig. 2 Leaf thickness of cigar tobacco‘CX-26 ’under different shading treatmentsNote： *， ** and *** indicate significant differences at P<0.05， P<0.01 and P<0.001 levels， respectively. Different lowercase letters among treatments indicate significant differences at P<0.05 level.

Fig. 3 Leaf flatness of cigar tobacco‘CX-26’under different shading treatmentsNote： * and ** indicate significant differences at P<0.05 and P<0.01 levels， respectively. Different lowercase letters between different treatments of same leaf position indicate significant differences at P<0.05 level.

Fig. 4 Leaf density of cigar tobacco‘CX-26’under different shading treatmentsNote： * and *** indicate significant differences at P<0.05 and P<0.001 levels， respectively. Different lowercase letters between different treatments of same leaf position indicate significant differences at P<0.05 level.

Fig. 5 Thickness of main and branch veins of cigar tobacco‘CX-26’under different shading treatmentsNote：* indicates significant differences at P<0.05 level. Different lowercase letters between different treatments of same leaf position indicate significant differences at P<0.05 level.

Fig. 6 Proportion of leaf vein to leaf dry matter of cigar tobacco‘CX-26’under different shading treatmentsNote： * indicates significant differences at P<0.05 level. Different lowercase letters between different treatments of same leaf position indicate significant differences at P<0.05 level.

Fig. 7 Leaf SPAD values of cigar tobacco‘CX-26’under different shading treatmentsNote： *， ** and *** indicate significant differences at P<0.05， P<0.01 and P<0.001 levels， respectively. Different lowercase letters among treatments indicate significant differences at P<0.05 level. The error bar represents the standard deviation， n=6.

Fig. 8 Correlation between leaf uniformity related indexes of cigar tobacco‘CX-26’A：Upper leaf； B：Middle leaf. LT—Leaf thickness； LD—Leaf density； TMV—Thickness of mian veins； TBV—Thickness of branch veins； PDW—Proportion of leaf vein to leaf dry matter； LF— Leaf flatness； SPAD—SPAD value. *， ** and *** indicate significant correlations at P<0.05， P<0.01 and P<0.001 levels， respetively.

References 25

1	李爱军, 秦艳青, 代惠娟, 等. 国产雪茄烟叶科学发展刍议[J]. 中国烟草学报, 2012, 18 (1): 112-114.
	LI A J, QIN Y Q, DAI H J, et al.. On scientific development of China’s cigar leaf [J]. Acta Tab. Sin., 2012, 18(1): 112-114.
2	邓弋戈, 时向东. 雪茄茄衣品种生态适应性及栽培技术研究进展[J]. 江西农业学报, 2021, 33 (1): 60-66.
	DENG Y Y, SHI X D. Research progress in ecological adaptability and cultivation techniques of cigar wrappers [J]. Acta Agric. Jiangxi, 2021, 33 (1): 60-66.
3	秦艳青, 李爱军, 范静苑, 等. 优质雪茄茄衣生产技术探讨[J]. 江西农业学报, 2012, 24 (7): 101-103.
	QIN Y Q, LI A J, FAN J Y, et al.. Discussion on production technology of high-quality cigar wrapper [J]. Acta Agric. Jiangxi, 2012, 24 (7): 101-103.
4	蔡斌, 耿召良, 高华军, 等. 国产雪茄原料生产技术研究现状[J]. 中国烟草学报, 2019, 25 (6): 110-119.
	CAI B, GENG Z L, GAO H J, et al.. Research progress of production technologies of cigar tobaccos in China [J]. Acta Tab. Sin., 2019, 25 (6): 110-119.
5	顾少龙, 史宏志. 光照对烤烟生长发育及质量形成的影响研究进展[J]. 河南农业科学, 2010(5): 120-124.
6	易克, 徐向丽, 卢向阳, 等. 光对烟草生长发育、生理代谢活动及品质形成影响的研究进展[J]. 化学与生物工程, 2013, 30(5): 11-16.
	YI K, XU X L, LU X Y, et al.. Research progress on the effects of light on growth, physiological metabolic activity and quality formation of tobacco [J]. Chem. Bioeng., 2013, 30 (5): 11-16.
7	FAN X X, XU Z G, LIU X Y, et al.. Effects of light intensity on the growth and leaf development of young tomato plants grown under a combination of red and blue light [J]. Sci. Hortic., 2013, 153: 50-55.
8	吴晓颖, 高华军, 王晓琳, 等. 光照强度对雪茄烟叶片组织结构及内源激素含量的影响[J]. 中国烟草科学, 2021, 42(2): 37-42.
	WU X Y, GAO H J, WANG X L, et al.. Effects of light intensity on growth and development and endogenous hormone content of cigar leaves [J]. Chin. Tob. Sci., 2021, 42(2): 37-42.
9	杨兴洪, 陈翠容, 施培. 遮阴对棉花茎叶解剖结构的影响[J]. 山东农业大学学报 (自然科学版), 2000, 31(4): 373-377.
	YANG X H, CHEN C R, SHI P. Effects of shading on the anatomical structure of cotton leaf and stem [J]. J. Shandong Agric. Univ. (Nat. Sci.), 2000, 31(4): 373-377.
10	裴保华, 彭伟秀, 张东林. 富贵草耐阴性的研究[J]. 河北林学院学报, 1994, 9(3): 205-209.
	PEI B H, PENG W X, ZHANG D L. Studies on shade tolerance of Pachysandra terminalls [J]. J. Hebei For. Coll., 1994, 9(3): 205-209.
11	时向东, 文志强, 刘艳芳, 等. 遮阴对雪茄外包皮烟生长和光合特性的影响[J]. 西北植物学报, 2006, 26(8): 1718-1721.
	SHI X D, WEN Z Q, LIU Y F, et al.. Effects of shading on growth and photosynthetic capabilities of tobacco leaves of cigar-wrapper use [J]. Acta Bot. Bor-Occid. Sin., 2006, 26(8): 1718-1721.
12	时向东, 汪文杰, 顾会战, 等. 遮阴对雪茄外包烟叶光合和水分利用效率日变化的影响[J]. 河南科学, 2006, 24 (5): 672-675.
	SHI X D, WANG W J, GU H Z, et al.. Effects of shading on diurnal variation of leaf photosynthesis and water use efficiency for cigar rapper tobacco [J]. Henan Sci., 2006, 24(5): 672-675.
13	王义伟, 张方学. 不同产地香料烟烟叶化学成分与外观形态特征偏相关分析[J]. 湖北农业科学, 2013, 52(4): 3328-3332.
	WANG Y W, ZHANG F X. Partial correlation analysis on appearance morphological characteristics and chemical composition of oriental tobacco from different producing areas [J]. Hubei Agric. Sci., 2013, 52(4): 3328-3332.
14	蔡宪杰, 王信民, 尹启生. 成熟度与烟叶质量的量化关系研究[J]. 中国烟草学报, 2005, 11(4): 42-46.
	CAI X J, WANG X M, YIN Q S. Study on the quantitative relationship bet ween maturity and quality of tobacco leaf [J]. Acta Tab. Sin., 2005, 11(4): 42-46.
15	张苧元. 烟草叶片脉络性状及影响因素的研究[D]. 长沙: 湖南农业大学, 2009.
	ZHANG N Y. Study on characteristics of tobacco leaf veins and its impact factors [D]. Changsha: Hunan Agricultural University, 2009.
16	刘芳, 陈国胜, 张斌, 等. 基于SPAD值的烤烟上部叶成熟度分类烘烤对品质的影响[J]. 贵州农业科学, 2022, 50(10): 13-20.
	LIU F, CHEN G S, ZHANG B, et al.. Effects of classified curing according to maturity on quality of upper tobacco leaves based on SPAD value [J]. Guizhou Agric. Sci., 2022, 50(10): 13-20.
17	江力, 曹树青, 戴新宾, 等. 光强对烟草光合作用的影响[J]. 中国烟草学报, 2000, 6(4): 17-20.
	JIANG L, CAO S Q, DAI X B, et al.. Effect of different light intensity on photosynthesis of tobacco [J]. Acta Tab. Sin., 2000, 6(4): 17-20.
18	马一琼, 顾会战, 许自成, 等. 烟草光照因素的研究进展[J]. 江西农业学报, 2017, 29 (7): 98-103.
	MA Y Q, GU H Z, XU Z C, et al.. Research progress in light factors in tobacco [J]. Acta Agric. Jiangxi, 2017, 29(7): 98-103.
19	周方舟, 程昌新, 周冀衡, 等. 光照和紫外强度对烤烟生长及叶片组织结构的影响[J]. 天津农业科学, 2017, 23(4): 14-18.
	ZHOU F Z, CHENG C X, ZHOU J H, et al.. Effects of light and ultraviolet intensity on the growth and leaf structure of flue-cured tobacco [J]. Tianjin Agric. Sci., 2017, 23(4): 14-18.
20	宋丹妮, 林勇, 邓世媛, 等. 光照强度对不同品种烤烟生长发育与生理特性的影响[J]. 西南农业学报, 2016, 29(1): 174-179.
	SONG D N, LIN Y, DENG S Y, et al.. Effects of light intensity on growth and development and physiological characteristics of different flue-cured tobacco varieties [J]. Southwest China J. Agric. Sci., 2016, 29(1): 174-179.
21	刘国顺. 烟草栽培学[M]. 北京: 中国农业出版社, 2003: 294-299.
22	闫克玉, 赵铭钦. 烟草原料学[M]. 北京: 科学出版社, 2008: 306-313.
23	朱大恒, 张闪闪, 莫骏坚, 等. 光强对湘南烟草生育后期光合及生理特性的影响[J]. 江苏农业科学, 2018, 46(8): 81-84.
24	任晓春, 高华军, 张本强, 等. 不同雪茄烟品种生长发育对光强的响应差异[J]. 中国烟草科学, 2022, 43(3): 96-102.
	REN X C, GAO H J, ZHANG B Q, et al.. The different response of growth and development to light intensity in two cigar varieties [J]. Chin. Tob. Sci., 2022, 43(3): 96-102.
25	MILLA R, REICH P B. The scaling of leaf area and mass: the cost of light interception increases with leaf size [J]. Proc. Royal Soc. B: Biol. Sci., 2007, 274: 2109-2114.

[1]	Xingfu WU, Fangchan JIAO, Xuejun CHEN, Zhiyu FANG, Guanghai ZHANG, Yihan ZHANG, Yongping LI. Phenotypic Genetic Diversity Analysis of Leaf Shrinkage and Economic Traits of Flue-cured Tobacco for Germplasm Resources [J]. Journal of Agricultural Science and Technology, 2023, 25(10): 54-64.
[2]	Jingjuan GAO, Chenyu ZHU, Yuqin KE, Chaoyuan ZHENG, Chunying LI, Wenqing LI. Effects of Organic Fertilizer Application Period on Carbon and Nitrogen Metabolism in Flue-cured Tobacco Under Tobacco-Rice Rotation [J]. Journal of Agricultural Science and Technology, 2023, 25(9): 157-165.
[3]	Yongtao HU, Daibin WANG, Yiyin CHEN, Chao YANG, Linlin ZHENG, Hongzhi SHI, Jianan WANG. Research on Contribution of Different Maturation with Fresh Tobacco Quality to Flue-cured Tobacco Quality [J]. Journal of Agricultural Science and Technology, 2023, 25(8): 157-164.
[4]	Xiaoran WANG, Xiaoyu LI, Hui SUN, Haidong YU, Yongchun SHI. Transcriptome Analysis of Tobacco Leaves Under Boron Stress [J]. Journal of Agricultural Science and Technology, 2023, 25(8): 53-64.
[5]	Xingsheng YIN, Lingfeng BAO, Yongyu PU, Jiali SUN, Qing ZHANG, Haiping LI, Mingying YANG, Yueping LIN, Huaixin WANG, Yonghong HE, Peiwen YANG. Effects of Chemical Fertilizer Reduction Combined with Bio-organic Fertilization on Tobacco Soil Characteristics and Tobacco Bacterial Wilt Control [J]. Journal of Agricultural Science and Technology, 2023, 25(7): 122-131.
[6]	Wen ZHOU, Xiaoheng GUO, Rui XU, Xiaoli WANG, Huiwei NIU, Dan HAN, Huifang SHAO. Effects of Intercropping Pinellia ternata on Growth， Yield and Quality of Flue-cured Tobacco [J]. Journal of Agricultural Science and Technology, 2023, 25(7): 161-169.
[7]	Yingxue ZHU, Qi WANG, Xianfa MA, Yusheng JIAO, Jinxu GAO, Weijia MAO, Jia FU, Xuedong SUN, Ye YUAN. Leaf Color Characteristic Value and Nitrogen Diagnosis Model of Tobacco During Growth Period [J]. Journal of Agricultural Science and Technology, 2023, 25(7): 54-62.
[8]	Yongyan LIU, Zhengxiong SONG, Jiawei JIN, Jing WANG, Min XU, Junxue ZHOU, Zhanmin LI, Shimin ZHAO, Yunpeng FU, Xiaoyan DAI. Effects of Molybdenum and Zinc Nutrition on Physiological Characteristics and Quality of Flue-cured Tobacco [J]. Journal of Agricultural Science and Technology, 2023, 25(6): 216-224.
[9]	Xinyan LI, Mingyue ZHANG, Fangling WANG, Huijuan JIANG, Jun WANG, Qiu ZHONG, Mingqin ZHAO. Effect of Different Water Retention Agents on Mineral Elements Dynamic Changes During the Growth and Development of Cigar Leaves [J]. Journal of Agricultural Science and Technology, 2023, 25(5): 55-65.
[10]	Wenjun ZHAO, Jizhou YANG, Mei YIN, Jianfeng CHEN, Kaizheng XUE, Baowen HU, Libo FU, Wei WANG, Zhiyuan WANG, Yanxian YANG, Hua CHEN. Effects of Combined Application of Green Manure with Reduced Nitrogen Fertilizer on Yield and Quality of Flue-cured Tobacco [J]. Journal of Agricultural Science and Technology, 2023, 25(4): 189-196.
[11]	Yunfei LIU, Fengjie WEI, Maolin XIA, Zhaojin YU, Hao XIA, Chunyu YI, Jianbo CHANG, Xiaoming JI. Alleviative Effect of New Composite Hydrogels on Cadmium Stress Tobacco Seedlings [J]. Journal of Agricultural Science and Technology, 2023, 25(3): 188-197.
[12]	Geng LI, Yuanyuan ZHAO, Yuyuan CHENG, Jiang WU, Weidong DUAN, Guangting YIN, Qian LI, Chen CHEN, Fei ZHENG, Yuan LIU, Hongzhi SHI. Effects of Different Organic-inorganic Nitrogen Ratios on Soil Carbon and Nitrogen and Upper Leaf Quality in Nanyang Tobacco Area [J]. Journal of Agricultural Science and Technology, 2023, 25(1): 175-186.
[13]	Zhuwen LIU, Longfei YANG, Maolin LIU, Guotao JIA, Qian YAO, Yiqiong MA, Ting CUI, Xinling YANG, Yang CHEN, Liangkun CHENG. Effects of Different Soil Amendments on Soil Nutrients and Inherent Quality of Flue-cured Tobacco [J]. Journal of Agricultural Science and Technology, 2022, 24(11): 190-198.
[14]	Yuqin XIAO, Xiao LEI, Mingjin ZHANG, Yuangai ZHANG, Shan TANG, Hongfei JI, Chuan WANG, Cuiling MA, Yanqiu JING. Effects of Three Bacillus Agents on Seedling Growth of Flue-cured Tobacco [J]. Journal of Agricultural Science and Technology, 2022, 24(5): 85-92.
[15]	Tianwei PENG, Huiya XIE, Sijun LI, Yixuan LIU, Kaifeng SHUAI, Yuanyuan PENG, Qing WANG, Diqin LI. Effects of Sodium Dinitrate with Bacillus Sbutilis Complex on Growth and Physiological Indexes of Tobacco Seedlings [J]. Journal of Agricultural Science and Technology, 2022, 24(4): 154-161.