Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (8): 53-64.DOI: 10.13304/j.nykjdb.2021.0954
• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles Next Articles
Xiaoran WANG(), Xiaoyu LI, Hui SUN, Haidong YU, Yongchun SHI(
)
Received:
2020-11-08
Accepted:
2022-02-07
Online:
2023-08-20
Published:
2023-09-07
Contact:
Yongchun SHI
通讯作者:
石永春
作者简介:
王潇然 E-mail:xiaoranwang@henau.edu.cn;
基金资助:
CLC Number:
Xiaoran WANG, Xiaoyu LI, Hui SUN, Haidong YU, Yongchun SHI. Transcriptome Analysis of Tobacco Leaves Under Boron Stress[J]. Journal of Agricultural Science and Technology, 2023, 25(8): 53-64.
王潇然, 李笑语, 孙慧, 于海东, 石永春. 硼胁迫下烟草叶片转录组分析[J]. 中国农业科技导报, 2023, 25(8): 53-64.
基因编号 Gene ID | 基因描述 Gene description | 正向引物 Forward primer (5’-3’) | 反向引物 Reverse primer (5’-3’) | 产物长度Product size/bp |
---|---|---|---|---|
EU938079 | 肌动蛋白Actin | GGAAACATCGTCCTTAGTGGTG | AATCCAGACACTGAACTTGCGT | 141 |
LOC104239595 | 蛋白TIFY8 Protein TIFY 8 | TAATGTCCATCCCAACAAGGC | TTTAGGCGAACTATTTTCTCCAG | 127 |
LOC107823411 | 碱性 β-1,3-葡聚糖酶 Basic β-1,3-glucanase | ACTGGCACATCTTACCTAACCTC | CCAACAATGGGATCAACAAAC | 194 |
LOC107826794 | 内切几丁质酶B Endochitinase B | CTGCTGGCTTAGAGAACAAGGTAG | GCTCTTCCACAAGGTCCGTAG | 141 |
LOC104119660 | 乙酰辅酶A氧化酶2 Acyl-coenzyme A oxidase 2 | CTTGGAGGATTTGGTGCTTG | GTTTTAGGGCAGCACGAGAG | 142 |
LOC104094164 | 乌头酸水合酶 Aconitate hydratase | GCAAAGTATCTTATGGAGCGTG | CCCAGTCGGTATGTGAATCG | 168 |
LOC104087505 | FK506结合蛋白类似物异构体X2 FK506-binding protein 5-like isoform X2 | CAAAGGAAGGCAGACGGTG | GCGTCAAGAATCCCTGCTC | 125 |
LOC104242999 | 细胞色素P450 CYP72A219类似物 Cytochrome P450 CYP72A219 like | CATTGCCAGCAGGGATACAG | GCCATTTTTGCCTCTAACATTG | 211 |
LOC104236587 | α,α-海藻糖-磷酸合成酶11异构体 X2 α,α-trehalose-phosphate synthase 11 isoform X2 | GTCTTGGCTTCAGAGTTGTCG | CACTCGGGGTTTTGTCCAC | 156 |
Table 1 Primer sequences
基因编号 Gene ID | 基因描述 Gene description | 正向引物 Forward primer (5’-3’) | 反向引物 Reverse primer (5’-3’) | 产物长度Product size/bp |
---|---|---|---|---|
EU938079 | 肌动蛋白Actin | GGAAACATCGTCCTTAGTGGTG | AATCCAGACACTGAACTTGCGT | 141 |
LOC104239595 | 蛋白TIFY8 Protein TIFY 8 | TAATGTCCATCCCAACAAGGC | TTTAGGCGAACTATTTTCTCCAG | 127 |
LOC107823411 | 碱性 β-1,3-葡聚糖酶 Basic β-1,3-glucanase | ACTGGCACATCTTACCTAACCTC | CCAACAATGGGATCAACAAAC | 194 |
LOC107826794 | 内切几丁质酶B Endochitinase B | CTGCTGGCTTAGAGAACAAGGTAG | GCTCTTCCACAAGGTCCGTAG | 141 |
LOC104119660 | 乙酰辅酶A氧化酶2 Acyl-coenzyme A oxidase 2 | CTTGGAGGATTTGGTGCTTG | GTTTTAGGGCAGCACGAGAG | 142 |
LOC104094164 | 乌头酸水合酶 Aconitate hydratase | GCAAAGTATCTTATGGAGCGTG | CCCAGTCGGTATGTGAATCG | 168 |
LOC104087505 | FK506结合蛋白类似物异构体X2 FK506-binding protein 5-like isoform X2 | CAAAGGAAGGCAGACGGTG | GCGTCAAGAATCCCTGCTC | 125 |
LOC104242999 | 细胞色素P450 CYP72A219类似物 Cytochrome P450 CYP72A219 like | CATTGCCAGCAGGGATACAG | GCCATTTTTGCCTCTAACATTG | 211 |
LOC104236587 | α,α-海藻糖-磷酸合成酶11异构体 X2 α,α-trehalose-phosphate synthase 11 isoform X2 | GTCTTGGCTTCAGAGTTGTCG | CACTCGGGGTTTTGTCCAC | 156 |
Fig. 2 Morphological indexes of tobacco leaves in different treatmentsNote: Different uppercase and lowercase letters of same trait indicate significant differences between different treatments at P<0.01 and P<0.05 levels, respectively.
器官Organ | T0 | T2 |
---|---|---|
叶Leaf | 0.899±0.065 Aa | 6.523±0.135 Aa |
茎Stem | 0.869±0.026 Aa | 1.176±0.107 Ab |
根Root | 0.570±0.059 Bb | 0.886±0.011 Ab |
Table 2 Boron contents in different tissues of tobacco under different treatments
器官Organ | T0 | T2 |
---|---|---|
叶Leaf | 0.899±0.065 Aa | 6.523±0.135 Aa |
茎Stem | 0.869±0.026 Aa | 1.176±0.107 Ab |
根Root | 0.570±0.059 Bb | 0.886±0.011 Ab |
性状Trait | T0 | T2 |
---|---|---|
可溶性糖含量Soluble sugar content | 8.165±0.167 Aa | 4.711±0.109 Bb |
葡萄糖含量Glucose content | 0.864±0.032 Aa | 0.871±0.051 Aa |
果糖含量Fructose content | 1.526±0.043 Aa | 1.755±0.028 Bb |
蔗糖含量Sucrose content | 4.726±0.031 Aa | 1.815±0.019 Bb |
淀粉含量Starch content | 46.551±0.143 Aa | 22.653±0.127 Bb |
叶绿素a含量Chlorophyll a content | 1.746±0.028 Aa | 1.632±0.019 Aa |
叶绿素b含量Chlorophyll b content | 0.800±0.009 Aa | 0.741±0.013 Aa |
类胡萝卜素含量Carotenoids content | 0.295±0.025 Aa | 0.265±0.008 Aa |
纤维素含量Cellulose content | 20.685±0.236 Aa | 13.24±0.433 Bb |
果胶含量Pectin content | 46.313±0.563 Aa | 30.500±1.250 Bb |
脯氨酸含量Proline content | 15.477±0.349 Aa | 24.488±0.988 Bb |
蛋白质含量Protein content | 47.836±2.506 Aa | 67.540±1.481 Bb |
过氧化氢含量H2O2 content | 0.450±0.120 Aa | 2.339±0.096 Bb |
Table 3 Physiological indexes of tobacco leaves under different treatments
性状Trait | T0 | T2 |
---|---|---|
可溶性糖含量Soluble sugar content | 8.165±0.167 Aa | 4.711±0.109 Bb |
葡萄糖含量Glucose content | 0.864±0.032 Aa | 0.871±0.051 Aa |
果糖含量Fructose content | 1.526±0.043 Aa | 1.755±0.028 Bb |
蔗糖含量Sucrose content | 4.726±0.031 Aa | 1.815±0.019 Bb |
淀粉含量Starch content | 46.551±0.143 Aa | 22.653±0.127 Bb |
叶绿素a含量Chlorophyll a content | 1.746±0.028 Aa | 1.632±0.019 Aa |
叶绿素b含量Chlorophyll b content | 0.800±0.009 Aa | 0.741±0.013 Aa |
类胡萝卜素含量Carotenoids content | 0.295±0.025 Aa | 0.265±0.008 Aa |
纤维素含量Cellulose content | 20.685±0.236 Aa | 13.24±0.433 Bb |
果胶含量Pectin content | 46.313±0.563 Aa | 30.500±1.250 Bb |
脯氨酸含量Proline content | 15.477±0.349 Aa | 24.488±0.988 Bb |
蛋白质含量Protein content | 47.836±2.506 Aa | 67.540±1.481 Bb |
过氧化氢含量H2O2 content | 0.450±0.120 Aa | 2.339±0.096 Bb |
酶Enzyme | T0 | T2 |
---|---|---|
超氧化物歧化酶SOD | 543.478±13.587 Aa | 260.870±5.435 Bb |
过氧化物酶POD | 338.750±9.656 Bb | 555.000±21.530 Aa |
蔗糖合成酶SS | 14.356±0.422 Aa | 16.044±0.826 Aa |
蔗糖磷酸合成酶SPS | 12.244±0.377 Ab | 14.778±0.491 Aa |
Table 4 Enzyme activities in tobacco leaves of different treatments
酶Enzyme | T0 | T2 |
---|---|---|
超氧化物歧化酶SOD | 543.478±13.587 Aa | 260.870±5.435 Bb |
过氧化物酶POD | 338.750±9.656 Bb | 555.000±21.530 Aa |
蔗糖合成酶SS | 14.356±0.422 Aa | 16.044±0.826 Aa |
蔗糖磷酸合成酶SPS | 12.244±0.377 Ab | 14.778±0.491 Aa |
Fig. 3 KEGG enrichment analysis of DEGs responding to boron toxicityNote: The value on the right side of the column represents the percentage to total genes.
基因编号 Gene ID | 基因描述 Gene description | log2比值 log2 ratio(T2/T0.05) | P值 P value |
---|---|---|---|
LOC104086800 | 内切葡聚糖酶11类似物Endoglucanase 11 like | 2.114 7 | 1.26e-05 |
LOC104216009 | 内切葡聚糖酶18类似物Endoglucanase 18 like | 1.150 7 | 1.65e-04 |
LOC104116779 | 内切葡聚糖酶6类似物Endoglucanase 6 like | -1.051 9 | 6.67e-05 |
LOC104215840 | 内切葡聚糖酶6类似物Endoglucanase 6 like | -1.212 0 | 4.32e-06 |
LOC104118953 | 果胶酯酶类似物Pectinesterase like | 1.921 4 | 1.46e-04 |
LOC104239567 | 叶绿体β-淀粉酶3 β-amylase 3, chloroplastic-like | 1.791 0 | 3.04e-09 |
LOC104236587 | 可能α,α-海藻糖磷酸合成酶11异构体X1 Probable α, α-trehalose -phosphate synthase 11 isoform X1 | 1.309 1 | 7.70e-05 |
LOC104210063 | 可能β-D-木糖苷酶2 Probable β-D-xylosidase 2 | 1.265 7 | 1.96e-04 |
LOC104228982 | 可能β-D-木糖苷酶5 Probable β-D-xylosidase 5 | -1.617 2 | 1.08e-04 |
LOC107800135 | 海藻糖酶Trehalase | -1.156 2 | 3.47e-05 |
LOC104110715 | β-葡糖苷酶 13类似物异构体 X1 β-glucosidase 13-like isoform X1 | -1.651 2 | 1.04e-07 |
LOC104218490 | 巢菜苷水解酶类似物Vicianin hydrolase-like | -1.687 4 | 2.04e-04 |
LOC104246036 | UDP-葡萄糖 6-脱氢酶4-类似物 UDP-glucose 6-dehydrogenase 4-like | -1.945 5 | 1.36e-05 |
LOC104087120 | 可能半乳糖醛糖基转移酶类似物1 Probable galacturonosyltransferase-like 1 | -2.140 6 | 4.82e-06 |
LOC104117285 | 可能为果胶酯酶/果胶酯酶抑制剂51异构体X1 Probable pectinesterase/pectinesterase inhibitor 51 isoform X1 | -2.217 8 | 6.46e-05 |
Table 5 Genes related to starch and sucrose metabolic pathways responding to boron toxicity
基因编号 Gene ID | 基因描述 Gene description | log2比值 log2 ratio(T2/T0.05) | P值 P value |
---|---|---|---|
LOC104086800 | 内切葡聚糖酶11类似物Endoglucanase 11 like | 2.114 7 | 1.26e-05 |
LOC104216009 | 内切葡聚糖酶18类似物Endoglucanase 18 like | 1.150 7 | 1.65e-04 |
LOC104116779 | 内切葡聚糖酶6类似物Endoglucanase 6 like | -1.051 9 | 6.67e-05 |
LOC104215840 | 内切葡聚糖酶6类似物Endoglucanase 6 like | -1.212 0 | 4.32e-06 |
LOC104118953 | 果胶酯酶类似物Pectinesterase like | 1.921 4 | 1.46e-04 |
LOC104239567 | 叶绿体β-淀粉酶3 β-amylase 3, chloroplastic-like | 1.791 0 | 3.04e-09 |
LOC104236587 | 可能α,α-海藻糖磷酸合成酶11异构体X1 Probable α, α-trehalose -phosphate synthase 11 isoform X1 | 1.309 1 | 7.70e-05 |
LOC104210063 | 可能β-D-木糖苷酶2 Probable β-D-xylosidase 2 | 1.265 7 | 1.96e-04 |
LOC104228982 | 可能β-D-木糖苷酶5 Probable β-D-xylosidase 5 | -1.617 2 | 1.08e-04 |
LOC107800135 | 海藻糖酶Trehalase | -1.156 2 | 3.47e-05 |
LOC104110715 | β-葡糖苷酶 13类似物异构体 X1 β-glucosidase 13-like isoform X1 | -1.651 2 | 1.04e-07 |
LOC104218490 | 巢菜苷水解酶类似物Vicianin hydrolase-like | -1.687 4 | 2.04e-04 |
LOC104246036 | UDP-葡萄糖 6-脱氢酶4-类似物 UDP-glucose 6-dehydrogenase 4-like | -1.945 5 | 1.36e-05 |
LOC104087120 | 可能半乳糖醛糖基转移酶类似物1 Probable galacturonosyltransferase-like 1 | -2.140 6 | 4.82e-06 |
LOC104117285 | 可能为果胶酯酶/果胶酯酶抑制剂51异构体X1 Probable pectinesterase/pectinesterase inhibitor 51 isoform X1 | -2.217 8 | 6.46e-05 |
基因编号 Gene ID | 基因描述 Gene description | log2 (T2/T0.05) | P值 P value |
---|---|---|---|
LOC104110787 | 长链酰基辅酶A合成酶2 Long chain acyl-CoA synthetase 2 | -1.176 5 | 2.08e-04 |
LOC104119660 | 酰基辅酶A氧化酶2, 过氧化物酶体 Acyl-coenzyme A oxidase 2, peroxisomal | 2.081 6 | 7.46e-12 |
LOC104214086 | 3-酮乙基-辅酶A合成酶6类似物 3-ketoacyl-CoA synthase 6 like | -1.005 9 | 1.71e-04 |
LOC104094697 | 3-酮乙基-辅酶A合成酶6 3-ketoacyl-CoA synthase 6 | -1.152 3 | 1.92e-05 |
LOC104228778 | 3-酮乙基-辅酶A合成酶19类似物 3-ketoacyl-CoA synthase 19-like | -1.956 0 | 3.65e-06 |
LOC104218838 | 3-酮乙基-辅酶A合成酶19类似物 3-ketoacyl-CoA synthase 19-like | -2.841 2 | 4.01e-04 |
LOC104114213 | 超长链烯脂酰辅酶A还原酶类似物 Very long chain enoyl CoA reductase like | 1.901 3 | 4.06e-05 |
LOC104089343 | 乙酰辅酶A羧化酶1类似物 Acetyl-CoA carboxylase 1-like | -1.050 0 | 6.13e-05 |
LOC104245391 | 3-酮二氢鞘氨醇还原酶类似物异构体X2 3-ketodihydrosphingosine reductase-like isoform X2 | -1.656 1 | 2.44e-07 |
Table 6 Fatty acid metabolic related genes that responding to boron toxicity
基因编号 Gene ID | 基因描述 Gene description | log2 (T2/T0.05) | P值 P value |
---|---|---|---|
LOC104110787 | 长链酰基辅酶A合成酶2 Long chain acyl-CoA synthetase 2 | -1.176 5 | 2.08e-04 |
LOC104119660 | 酰基辅酶A氧化酶2, 过氧化物酶体 Acyl-coenzyme A oxidase 2, peroxisomal | 2.081 6 | 7.46e-12 |
LOC104214086 | 3-酮乙基-辅酶A合成酶6类似物 3-ketoacyl-CoA synthase 6 like | -1.005 9 | 1.71e-04 |
LOC104094697 | 3-酮乙基-辅酶A合成酶6 3-ketoacyl-CoA synthase 6 | -1.152 3 | 1.92e-05 |
LOC104228778 | 3-酮乙基-辅酶A合成酶19类似物 3-ketoacyl-CoA synthase 19-like | -1.956 0 | 3.65e-06 |
LOC104218838 | 3-酮乙基-辅酶A合成酶19类似物 3-ketoacyl-CoA synthase 19-like | -2.841 2 | 4.01e-04 |
LOC104114213 | 超长链烯脂酰辅酶A还原酶类似物 Very long chain enoyl CoA reductase like | 1.901 3 | 4.06e-05 |
LOC104089343 | 乙酰辅酶A羧化酶1类似物 Acetyl-CoA carboxylase 1-like | -1.050 0 | 6.13e-05 |
LOC104245391 | 3-酮二氢鞘氨醇还原酶类似物异构体X2 3-ketodihydrosphingosine reductase-like isoform X2 | -1.656 1 | 2.44e-07 |
基因编号 Gene ID | 基因描述 Gene description | log2比值 log2 ratio(T2/T0.05) | P值 P value |
---|---|---|---|
LOC104235161 | 致病相关蛋白1碱性形式类似物 Basic form of pathogenesis related protein 1 like (PR1) | 3.453 6 | 3.79e-06 |
LOC104116631 | 推测抗病蛋白At4g33300 Putative disease resistance protein At4g33300 | 1.447 4 | 2.75e-08 |
LOC104234903 | 推测抗病蛋白RPP13类似物蛋白1异构体X1 Putative disease resistance RPP13-like protein 1 isoform X1 | -1.043 4 | 2.22e-04 |
LOC104233182 | 推测抗病蛋白RPM1类似物 Putative disease resistance protein RPM1 like | 1.529 6 | 6.53e-05 |
LOC104216132 | 富含半胱氨酸受体样蛋白激酶3 Cysteine rich receptor like protein kinase 3 | 1.230 8 | 3.05e-04 |
LOC104217774 | G型植物凝血素S受体类似物丝氨酸/苏氨酸蛋白激酶At4g27290异构体X1 G-type lectin S-receptor-like serine/threonine protein kinase At4g27290 isoform X1 | 5.164 6 | 1.92e-10 |
LOC104241125 | G型植物凝血素S受体类似物丝氨酸/苏氨酸蛋白激酶At4g27290异构体X1 G-type lectin S-receptor-like serine/threonine protein kinase At4g27290 isoform X1 | 1.690 4 | 3.08e-04 |
LOC104246103 | G型植物凝血素S受体类似物丝氨酸/苏氨酸蛋白激酶At4g27290异构体X1 G-type lectin S-receptor-like serine/threonine protein kinase At4g27290 isoform X1 | 1.365 2 | 3.65e-04 |
LOC104096948 | G型植物凝血素S受体类似物丝氨酸/苏氨酸蛋白激酶At5g24080异构体X1 G-type lectin S-receptor-like serine/threonine protein kinase At5g24080 isoform X1 | -2.414 8 | 3.69e-08 |
LOC104103311 | 富含脯氨酸受体样蛋白激酶PERK9 Proline-rich receptor-like protein kinase PERK9 | 2.716 3 | 8.75e-10 |
LOC104236563 | 磷酸烯醇丙酮酸羧化酶激酶1类似物 Phosphoenolpyruvate carboxylase kinase 1-like | 1.289 7 | 4.53e-05 |
LOC104091723 | 磷酸烯醇丙酮酸羧化酶激酶1类似物 Phosphoenolpyruvate carboxylase kinase 1-like | 1.470 1 | 5.79e-08 |
LOC104223256 | PTI1类似物酪氨酸蛋白激酶At3g15890类似物 PTI1 like tyrosine protein kinase At3g15890 like | 1.076 4 | 2.61e-04 |
LOC104084644 | 推测受体蛋白激酶ZmPK1类似物 Putative receptor protein kinase ZmPK1 like | 2.982 9 | 1.73e-05 |
LOC104216857 | 推测丝氨酸/苏氨酸蛋白激酶异构体X2 Putative serine/threonine protein kinase isoform X2 | 1.676 3 | 1.93e-08 |
LOC104112558 | 聚半乳糖醛酸酶抑制剂类似物 Polygalacturonase inhibitor like | 4.603 1 | 1.57e-04 |
LOC104239595 | 蛋白TIFY8 Protein TIFY 8 | 1.851 0 | 3.50e-04 |
LOC104111058 | 呼吸爆发氧化酶同源蛋白E类似物 Respiratory burst oxidase homolog protein E like | -2.108 6 | 1.06e-04 |
LOC104093573 | 转录因子PIF1类似物 Transcription factor PIF1 like | 1.445 1 | 1.57e-05 |
Table 7 Genes responding to boron toxicity
基因编号 Gene ID | 基因描述 Gene description | log2比值 log2 ratio(T2/T0.05) | P值 P value |
---|---|---|---|
LOC104235161 | 致病相关蛋白1碱性形式类似物 Basic form of pathogenesis related protein 1 like (PR1) | 3.453 6 | 3.79e-06 |
LOC104116631 | 推测抗病蛋白At4g33300 Putative disease resistance protein At4g33300 | 1.447 4 | 2.75e-08 |
LOC104234903 | 推测抗病蛋白RPP13类似物蛋白1异构体X1 Putative disease resistance RPP13-like protein 1 isoform X1 | -1.043 4 | 2.22e-04 |
LOC104233182 | 推测抗病蛋白RPM1类似物 Putative disease resistance protein RPM1 like | 1.529 6 | 6.53e-05 |
LOC104216132 | 富含半胱氨酸受体样蛋白激酶3 Cysteine rich receptor like protein kinase 3 | 1.230 8 | 3.05e-04 |
LOC104217774 | G型植物凝血素S受体类似物丝氨酸/苏氨酸蛋白激酶At4g27290异构体X1 G-type lectin S-receptor-like serine/threonine protein kinase At4g27290 isoform X1 | 5.164 6 | 1.92e-10 |
LOC104241125 | G型植物凝血素S受体类似物丝氨酸/苏氨酸蛋白激酶At4g27290异构体X1 G-type lectin S-receptor-like serine/threonine protein kinase At4g27290 isoform X1 | 1.690 4 | 3.08e-04 |
LOC104246103 | G型植物凝血素S受体类似物丝氨酸/苏氨酸蛋白激酶At4g27290异构体X1 G-type lectin S-receptor-like serine/threonine protein kinase At4g27290 isoform X1 | 1.365 2 | 3.65e-04 |
LOC104096948 | G型植物凝血素S受体类似物丝氨酸/苏氨酸蛋白激酶At5g24080异构体X1 G-type lectin S-receptor-like serine/threonine protein kinase At5g24080 isoform X1 | -2.414 8 | 3.69e-08 |
LOC104103311 | 富含脯氨酸受体样蛋白激酶PERK9 Proline-rich receptor-like protein kinase PERK9 | 2.716 3 | 8.75e-10 |
LOC104236563 | 磷酸烯醇丙酮酸羧化酶激酶1类似物 Phosphoenolpyruvate carboxylase kinase 1-like | 1.289 7 | 4.53e-05 |
LOC104091723 | 磷酸烯醇丙酮酸羧化酶激酶1类似物 Phosphoenolpyruvate carboxylase kinase 1-like | 1.470 1 | 5.79e-08 |
LOC104223256 | PTI1类似物酪氨酸蛋白激酶At3g15890类似物 PTI1 like tyrosine protein kinase At3g15890 like | 1.076 4 | 2.61e-04 |
LOC104084644 | 推测受体蛋白激酶ZmPK1类似物 Putative receptor protein kinase ZmPK1 like | 2.982 9 | 1.73e-05 |
LOC104216857 | 推测丝氨酸/苏氨酸蛋白激酶异构体X2 Putative serine/threonine protein kinase isoform X2 | 1.676 3 | 1.93e-08 |
LOC104112558 | 聚半乳糖醛酸酶抑制剂类似物 Polygalacturonase inhibitor like | 4.603 1 | 1.57e-04 |
LOC104239595 | 蛋白TIFY8 Protein TIFY 8 | 1.851 0 | 3.50e-04 |
LOC104111058 | 呼吸爆发氧化酶同源蛋白E类似物 Respiratory burst oxidase homolog protein E like | -2.108 6 | 1.06e-04 |
LOC104093573 | 转录因子PIF1类似物 Transcription factor PIF1 like | 1.445 1 | 1.57e-05 |
基因编号 Gene ID | 基因描述 Gene description | 激素 Hormone | log2比值 log2 ratio(T2/T0.05) | P值 P value |
---|---|---|---|---|
LOC104096661 | 乙烯应答转录因子ERF003类似物异构体X1 Ethylene responsive transcription factor ERF003 like isoform X1 | 乙烯Ethylene | 4.123 8 | 3.01e-09 |
LOC107784797 | 乙烯受体同源物Ethylene receptor homolog | 乙烯Ethylene | 1.941 7 | 3.78e-05 |
LOC104239595 | 蛋白TIFY8 Protein TIFY 8 | 茉莉酸JA | 1.850 9 | 0.000 350 |
LOC104100899 | 生长激素响应蛋白IAA26类似物 Auxin-responsive protein IAA26-like | 生长素Auxin | 1.602 6 | 4.37e-06 |
LOC104243881 | 生长激素响应因子9类似物(ARF9) Auxin response factor 9 like (ARF9) | 生长素Auxin | 1.025 0 | 0.000 401 |
LOC104085449 | 转运抑制响应蛋白1(TIR1) Protein transport inhibitor response 1(TIR1) | 生长素Auxin | -1.529 0 | 0.000 392 |
LOC104093573 | 转录因子PIF1类似物 Transcription factor PIF1 like | 脱落酸、赤霉素ABA, GA | 1.445 0 | 1.57e-05 |
LOC104086506 | 蛋白质磷酸酶2C 37类似物 Protein phosphatase 2C 37-like | 脱落酸ABA | -1.980 2 | 0.000 198 |
LOC107782457 | TGACG 序列特异性DNA结合蛋白TGA2.1 TGACG-sequence-specific DNA binding protein TGA2.1 | 水杨酸SA | 1.237 7 | 1.41e-05 |
LOC107782457 | TGACG 序列特异性DNA结合蛋白TGA2.1 TGACG-sequence-specific DNA binding protein TGA2.1 | 水杨酸SA | 1.130 9 | 2.60e-05 |
LOC104235161 | 致病相关蛋白1碱性形态类似物(PR1) Basic form of pathogenesis related protein 1 like (PR1) | 水杨酸SA | 3.453 6 | 3.79e-06 |
LOC104114105 | BES1/BZR1同源蛋白4类似物 BES1/BZR1 homolog protein 4-like | 油菜素内酯Brassinosteroid | -2.293 1 | 2.75e-05 |
Table 8 Genes related to hormone signal transduction in plants responding to boron toxicity
基因编号 Gene ID | 基因描述 Gene description | 激素 Hormone | log2比值 log2 ratio(T2/T0.05) | P值 P value |
---|---|---|---|---|
LOC104096661 | 乙烯应答转录因子ERF003类似物异构体X1 Ethylene responsive transcription factor ERF003 like isoform X1 | 乙烯Ethylene | 4.123 8 | 3.01e-09 |
LOC107784797 | 乙烯受体同源物Ethylene receptor homolog | 乙烯Ethylene | 1.941 7 | 3.78e-05 |
LOC104239595 | 蛋白TIFY8 Protein TIFY 8 | 茉莉酸JA | 1.850 9 | 0.000 350 |
LOC104100899 | 生长激素响应蛋白IAA26类似物 Auxin-responsive protein IAA26-like | 生长素Auxin | 1.602 6 | 4.37e-06 |
LOC104243881 | 生长激素响应因子9类似物(ARF9) Auxin response factor 9 like (ARF9) | 生长素Auxin | 1.025 0 | 0.000 401 |
LOC104085449 | 转运抑制响应蛋白1(TIR1) Protein transport inhibitor response 1(TIR1) | 生长素Auxin | -1.529 0 | 0.000 392 |
LOC104093573 | 转录因子PIF1类似物 Transcription factor PIF1 like | 脱落酸、赤霉素ABA, GA | 1.445 0 | 1.57e-05 |
LOC104086506 | 蛋白质磷酸酶2C 37类似物 Protein phosphatase 2C 37-like | 脱落酸ABA | -1.980 2 | 0.000 198 |
LOC107782457 | TGACG 序列特异性DNA结合蛋白TGA2.1 TGACG-sequence-specific DNA binding protein TGA2.1 | 水杨酸SA | 1.237 7 | 1.41e-05 |
LOC107782457 | TGACG 序列特异性DNA结合蛋白TGA2.1 TGACG-sequence-specific DNA binding protein TGA2.1 | 水杨酸SA | 1.130 9 | 2.60e-05 |
LOC104235161 | 致病相关蛋白1碱性形态类似物(PR1) Basic form of pathogenesis related protein 1 like (PR1) | 水杨酸SA | 3.453 6 | 3.79e-06 |
LOC104114105 | BES1/BZR1同源蛋白4类似物 BES1/BZR1 homolog protein 4-like | 油菜素内酯Brassinosteroid | -2.293 1 | 2.75e-05 |
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