Comprehensive Evaluation and Screening of Drought Resistance of 86 Millet Germplasm Resources During Germination Period

doi:10.13304/j.nykjdb.2024.0515

Abstract

Abstract:

The seed germination stage is the most susceptible to external environmental factors. In order to screen millet germplasm resources with strong drought resistance at germination stage， 86 millet germplasms from Inner Mongolia were as materials. 2 treatments were set including PEG-6000 with mass volume fraction of 0% and 20%， 7 traits were determined including germination potential （GP）， germination rate （GR）， germination index （GI）， root length （RL）， bud length （BL）， root bud ratio （RBR） and fresh weight （FW）. The drought resistance of different millet germplasms at germination stage was evaluated by comprehensive evaluation method. The results showed that the variation coefficients of 7 traits were 4.00%~37.44%， and the drought resistance coefficients were 20.06%~33.84%. Correlation analysis showed that there were correlations among the drought resistance coefficients of traits with the highest correlation （0.92） between the germination index and the germination rate. Principal component analysis got 5 principal components with the cumulative contribution rate of 99.25%. The cluster analysis showed that 86 millet germplasms were divided into 5 groups. And 12 millet germplasms were screened with high drought tolerance， including CMG01， CMG04， CMG05， CMG20， CMG30， CMG37， CGK9， GG2， JMK10， CMG58， CMG60， CMG62. The prediction model for evaluating drought resistance of millet germination was developed using stepwise regression analysis， Y=-0.225+0.838x₃+0.170x₄+0.219x₅+0.116x₆， which x₃， x₄， x₅， x₆ were GI， RL， BL and FW， respectively， and they could be as indicators of drought resistance during the germination period of millet grains. Above results provided the germplasm basis and theoretical reference for breeding new varieties and the drought resistance mechanism of millet.

Key words: foxtail millet, seedling stage, drought stress tolerance, the comprehensive assessment

摘要：

种子萌发阶段是对外界环境最敏感阶段。为筛选萌发期具有较强抗旱性的谷子种质资源，以内蒙古地区86份谷子种质为研究对象，分别设置质量体积分数0%和20% PEG-6000干旱胁迫处理，测定不同处理下各种质萌发期的发芽势、发芽率、萌发指数、根长、芽长、芽根比、鲜重共7个性状，并采用综合评价法对不同谷子种质萌发期的抗旱性进行评价。结果表明，干旱胁迫影响谷子种子萌发，7个性状的变异系数为4.00%~37.44%，抗旱系数的变异系数为20.06%~33.84%；各指标的抗旱系数间均呈现出一定的相关性，其中萌发指数与发芽率的相关性最强，相关系数为0.92。主成分分析提取出5个主成分，累计贡献率达99.25%。聚类分析将86份谷子种质划分为5个类群，筛选出高抗旱种质12份，分别为CMG01、CMG04、CMG05、CMG20、CMG30、CMG37、赤谷K9、公谷2号、金苗K10、CMG58、CMG60、CMG62。通过逐步回归分析建立谷子萌发期抗旱性评价预测模型为：Y=-0.225+0.838x₃+0.170x₄+0.219x₅+0.116x₆，x₃、x₄、x₅、x₆分别为萌发指数、根长、芽长、鲜重，这4个性状可作为谷子萌发期抗旱性的鉴定指标。以上研究结果为谷子抗旱新品种培育和抗旱机理研究提供种质基础和理论参考。

关键词: 谷子, 萌发期, 抗旱性, 综合评价

CLC Number:

S515

Xinwei XUE, Dan LIU, Shi ZHANG, Wenyu HAN, Ankang MU, Zhikun YU, Fan YANG, Yahui WEN, Jialin ZHANG, Yongping ZHANG, Xianrui WANG. Comprehensive Evaluation and Screening of Drought Resistance of 86 Millet Germplasm Resources During Germination Period[J]. Journal of Agricultural Science and Technology, 2025, 27(6): 39-51.

薛新伟, 刘丹, 张姼, 韩雯毓, 穆安康, 于智坤, 杨帆, 温雅辉, 张家林, 张永平, 王显瑞. 86份谷子种质资源萌发期抗旱性综合评价及筛选[J]. 中国农业科技导报, 2025, 27(6): 39-51.

Figures/Tables 13

References 34

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处理 Treatment	参数 Parameter	发芽势 GP/%	发芽率 GR/%	萌发指数 GI/%	根长 RL/cm	芽长 BL/cm	根芽比 RBR	鲜重 FW/mg
对照CK	最大值Max	1.00	1.00	1.00	9.40	7.00	1.30	19.97
	最小值Min	0.33	0.78	0.54	3.27	2.57	0.37	5.00
	均值Ave	0.91 a	0.96 a	0.91 a	6.55 a	3.98 a	0.63 a	13.07 a
	标准差SD	0.10	0.04	0.08	1.30	0.79	0.17	3.29
	变异系数CV/%	11.30	4.00	9.24	19.78	19.72	26.74	25.13
干旱 Drought	最大值Max	0.73	0.83	0.68	6.23	3.43	1.08	16.03
	最小值Min	0.05	0.13	0.08	1.10	0.73	0.23	0.97
	均值Ave	0.43 b	0.57 b	0.42 b	3.60 b	1.74 b	0.50 b	8.48 b
	标准差SD	0.14	0.15	0.12	0.92	0.56	0.17	3.18
	变异系数CV/%	33.86	25.68	28.74	25.49	32.14	33.05	37.44

处理 Treatment	参数 Parameter	发芽势 GP/%	发芽率 GR/%	萌发指数 GI/%	根长 RL/cm	芽长 BL/cm	根芽比 RBR	鲜重 FW/mg
对照CK	最大值Max	1.00	1.00	1.00	9.40	7.00	1.30	19.97
	最小值Min	0.33	0.78	0.54	3.27	2.57	0.37	5.00
	均值Ave	0.91 a	0.96 a	0.91 a	6.55 a	3.98 a	0.63 a	13.07 a
	标准差SD	0.10	0.04	0.08	1.30	0.79	0.17	3.29
	变异系数CV/%	11.30	4.00	9.24	19.78	19.72	26.74	25.13
干旱 Drought	最大值Max	0.73	0.83	0.68	6.23	3.43	1.08	16.03
	最小值Min	0.05	0.13	0.08	1.10	0.73	0.23	0.97
	均值Ave	0.43 b	0.57 b	0.42 b	3.60 b	1.74 b	0.50 b	8.48 b
	标准差SD	0.14	0.15	0.12	0.92	0.56	0.17	3.18
	变异系数CV/%	33.86	25.68	28.74	25.49	32.14	33.05	37.44

参数 Parameter	发芽势 GP	发芽率 GR	萌发指数 GI	根长 RL	芽长 BL	芽根比 RBR	鲜重 FW
最大值 Max	0.92	0.89	0.83	0.85	0.67	1.27	1.06
最小值 Min	0.05	0.14	0.10	0.25	0.19	0.38	0.19
均值 Ave	0.47	0.59	0.47	0.56	0.44	0.80	0.65
标准差 SD	0.16	0.15	0.14	0.13	0.12	0.16	0.19
变异系数 CV/%	33.84	25.83	30.27	22.81	26.44	20.06	29.09

参数 Parameter	发芽势 GP	发芽率 GR	萌发指数 GI	根长 RL	芽长 BL	芽根比 RBR	鲜重 FW
最大值 Max	0.92	0.89	0.83	0.85	0.67	1.27	1.06
最小值 Min	0.05	0.14	0.10	0.25	0.19	0.38	0.19
均值 Ave	0.47	0.59	0.47	0.56	0.44	0.80	0.65
标准差 SD	0.16	0.15	0.14	0.13	0.12	0.16	0.19
变异系数 CV/%	33.84	25.83	30.27	22.81	26.44	20.06	29.09

性状 Trait	发芽势 GP	发芽率 GR	萌发指数 GI	根长 RL	芽长 BL	根芽比 RBR
发芽率GR	0.736^**
萌发指数GI	0.916^**	0.885^**
根长RL	0.556^**	0.633^**	0.616^**
芽长BL	0.386^**	0.621^**	0.488^**	0.690^**
根芽比RBR	-0.092	0.099	-0.033	0.216^*	0.536^**
鲜重FW	0.522^**	0.698^**	0.599^**	0.613^**	0.711^**	0.263^*