Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (8): 216-224.DOI: 10.13304/j.nykjdb.2022.0317


Application of Multiplex PCR on Detection of Plant Pathogens

Zijian CAO1,2(), Yanhong QIU3, Shuang WANG4, Juan ZHAO1, Suyue ZHENG2, Guanghang QIAO1, Wentao QIN1()   

  1. 1.Institute of Plant Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China
    2.School of Landscape and Ecological Engineering,Hebei University of Engineering,Hebei Handan 056038,China
    3.Institute of Vegetable Science,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China
    4.Administrative Office of the Summer Palace,Beijing Municipal Administration Center of Parks,Beijing 100097,China
  • Received:2022-04-17 Accepted:2022-05-18 Online:2023-08-20 Published:2023-09-07
  • Contact: Wentao QIN


曹子健1,2(), 邱艳红3, 王爽4, 赵娟1, 郑素月2, 乔广行1, 秦文韬1()   

  1. 1.北京市农林科学院植物保护研究所,北京 100097
    2.河北工程大学园林与生态工程学院,河北 邯郸 056038
    3.北京市农林科学院蔬菜研究所,北京 100097
    4.北京市颐和园管理处,北京 100097
  • 通讯作者: 秦文韬
  • 作者简介:曹子健
  • 基金资助:


There are various plant pathogens, and climate change and globalization have intensified the spread of pathogens. Rapid and reliable diagnostic techniques for plant pathogens play important roles for the epidemic warning and scientific prevention of the disease. With the development of technology, multiplex PCR technology with the advantages of high accuracy and efficiency is widely used in many fields of the life sciences. The principle of this technical was briefly introduced, and the applications of multiplex PCR in the detection of plant pathogens including fungi, bacteria, viruses and nematodes were also reviewed. The existing problems and solutions of this technology were analyzed, and the development of this technology in the future was finally prospected.

Key words: multiplex PCR, plant pathogen, detection, application



关键词: 多重PCR, 植物病原物, 检测, 应用

CLC Number: