Application of Multiplex PCR on Detection of Plant Pathogens

doi:10.13304/j.nykjdb.2022.0317

Abstract

Abstract:

There are various plant pathogens， and climate change and globalization have intensified the spread of pathogens. Rapid and reliable diagnostic techniques for plant pathogens play important roles for the epidemic warning and scientific prevention of the disease. With the development of technology， multiplex PCR technology with the advantages of high accuracy and efficiency is widely used in many fields of the life sciences. The principle of this technical was briefly introduced， and the applications of multiplex PCR in the detection of plant pathogens including fungi， bacteria， viruses and nematodes were also reviewed. The existing problems and solutions of this technology were analyzed， and the development of this technology in the future was finally prospected.

Key words: multiplex PCR, plant pathogen, detection, application

摘要：

植物病原物种类繁多，且气候变化及全球化发展加剧了病原的扩散。快速可靠的植物病原诊断技术对病害的流行预警和防控管理具有重要作用。随着技术发展，多重PCR技术因具有精准、高效的特点而被广泛应用到生命科学的多个领域。对多重PCR技术的原理及其在植物病原真菌、细菌、病毒、线虫检测中的应用进行了系统综述，分析了该技术目前存在的问题及解决方式，并展望了该技术未来的发展前景。

关键词: 多重PCR, 植物病原物, 检测, 应用

CLC Number:

S432

Zijian CAO, Yanhong QIU, Shuang WANG, Juan ZHAO, Suyue ZHENG, Guanghang QIAO, Wentao QIN. Application of Multiplex PCR on Detection of Plant Pathogens[J]. Journal of Agricultural Science and Technology, 2023, 25(8): 216-224.

曹子健, 邱艳红, 王爽, 赵娟, 郑素月, 乔广行, 秦文韬. 多重PCR技术在植物病原物检测中的应用[J]. 中国农业科技导报, 2023, 25(8): 216-224.

Figures/Tables 2

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