Leaf Color Characteristic Value and Nitrogen Diagnosis Model of Tobacco During Growth Period

doi:10.13304/j.nykjdb.2021.1074

Abstract

Abstract:

In order to clarify the color characteristic parameters significantly related to the total nitrogen content of tobacco leaves， the relationship between the leaf color characteristic value and the total nitrogen content and chlorophyll （expressed by SPAD value） of tobacco leaves under different nitrogen application rates was analyzed， and the color characteristic value diagnosis model of total nitrogen content of tobacco leaves was established by multiple stepwise regression analysis. The results showed that， under different nitrogen application rates， the most sensitive period to the change of nitrogen content was fast growing period. There was a significant negative correlation （P<0.05） between color characteristic values （red， R； green， G； blue， B； lightness， L） and leaf SPAD values， which the correlation coefficient showed as r_R（0.728）>r_L （0.706）>r_G（0.649）>r_B（0.477）. There was significant correlation （P<0.05） between total nitrogen content and color characteristic values L， R， G， L/（R+G）， B/R， B/G， L/B， R+B， B+G， R+B+G+L， R/（G+B） and R+B/（R+B+G）. The nitrogen diagnosis model was established by multiple stepwise regression analysis using the characteristic values of leaf total nitrogen content and color characteristic values， which was significant at P<0.05 level. The model was verified， and the correlation between the measured value and the simulated value reached significant at P<0.01 level. The model of leaf color characteristic and leaf total nitrogen content of flue-cured tobacco could be used to guide the nitrogen nutrition diagnosis of flue-cured tobacco during the growth period， which provided a theoretical basis for smart agriculture in the future.

Key words: tobacco leaf, color characteristic value, nitrogen, chlorophyll

摘要：

为明确与烟叶全氮含量显著相关的颜色特征参数，利用多元逐步回归分析，建立烟叶全氮含量的颜色特征值诊断模型，分析不同氮肥用量下叶片颜色特征值与烟叶全氮含量及叶绿素（以SPAD值表示）的关系。结果表明，不同氮肥用量下，烤烟对氮素含量变化最敏感的时期为旺长期。颜色特征值［红色（red， R），绿色（green， G），蓝色（blue， B），亮度（lightness， L）］与叶片SPAD值之间呈显著负相关（P<0.05），其相关系数表现为r_R（0.728）>r_L（0.706）>r_G（0.649）>r_B（0.477）。烟叶全氮含量与颜色特征值L、R、G、L/（R+G）、B/R、B/G、L/B、R+B、B+G、R+B+G+L、R/（G+B）、R+B/（R+B+G）呈显著相关（P<0.05）。利用叶片全氮含量和颜色特征值进行多元逐步回归分析，构建氮素诊断模型，模型达到显著水平（P<0.05），对该模型进行验证，实测值与模拟值的相关性达到极显著水平（P<0.01）。因此，模型可用于指导烤烟生长期的氮素营养诊断，可方便、快捷地诊断烤烟叶片全氮含量，为智慧农业的发展提供理论依据。

关键词: 烟叶, 颜色特征值, 氮素, 叶绿素

CLC Number:

S572

Yingxue ZHU, Qi WANG, Xianfa MA, Yusheng JIAO, Jinxu GAO, Weijia MAO, Jia FU, Xuedong SUN, Ye YUAN. Leaf Color Characteristic Value and Nitrogen Diagnosis Model of Tobacco During Growth Period[J]. Journal of Agricultural Science and Technology, 2023, 25(7): 54-62.

朱莹雪, 王琪, 马献发, 焦玉生, 高金旭, 毛卫佳, 付佳, 孙雪岽, 元野. 烤烟生长期叶片颜色特征值及其氮素诊断模型[J]. 中国农业科技导报, 2023, 25(7): 54-62.

Figures/Tables 8

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处理 Treatment	纯氮施用量 Nitrogen application rate/（kg·hm^-2）
处理 Treatment	总氮 Total nitrogen	移栽前1 d 1 d before transplanting	移栽后58 d 58 d after transplanting
N0	0.0	0.0	0.0
N1	15.0	7.5	7.5
N2	30.0	15.0	15.0
N3	45.0	22.5	22.5
N4	60.0	30.0	30.0
N5	75.0	37.5	37.5
N6	90.0	45.0	45.0

处理 Treatment	纯氮施用量 Nitrogen application rate/（kg·hm^-2）
处理 Treatment	总氮 Total nitrogen	移栽前1 d 1 d before transplanting	移栽后58 d 58 d after transplanting
N0	0.0	0.0	0.0
N1	15.0	7.5	7.5
N2	30.0	15.0	15.0
N3	45.0	22.5	22.5
N4	60.0	30.0	30.0
N5	75.0	37.5	37.5
N6	90.0	45.0	45.0

指标 Index	TN	L	R	G	B	R/ （G+B）	L/ （R+G）	G/R	B/R	B/G	L/B	（R+B）/（R+B+G）	R+B	B+G
L	-0.575^**
R	-0.553^**	0.987^**
G	-0.597^**	0.991^**	0.960^**
B	-0.253	0.773^**	0.767^**	0.743^**
R/ （G+B）	-0.338^*	0.440^**	0.547^**	0.364^*	0.021
L/ （R+G）	0.473^**	-0.531^**	-0.585^**	-0.535^**	-0.209	-0.594^**
G/R	0.011	-0.268	-0.415^**	-0.145	-0.297	-0.755^**	0.344^*
B/R	0.508^**	-0.430^**	-0.460^**	-0.429^**	0.215	-0.813^**	0.618^**	0.234
B/G	0.503^**	-0.307^*	-0.267	-0.364^*	0.351^*	-0.455^**	0.460^**	-0.239	0.888^**
L/B	-0.460^**	0.346^*	0.337^*	0.373^*	-0.324^*	0.633^**	-0.464^**	0.024	-0.954^**	-0.964^**
R+B/ （R+B+G）	0.355^*	-0.082	0.025	-0.190	0.412^**	0.073	0.153	-0.708^**	0.521^**	0.855^**	-0.716^**
R+B	-0.473^**	0.964^**	0.971^**	0.934^**	0.899^**	0.382^*	-0.478^**	-0.395^**	-0.234	-0.051	0.108	0.172
B+G	-0.512^**	0.976^**	0.952^**	0.971^**	0.881^**	0.265	-0.452^**	-0.208	-0.226	-0.132	0.148	0.013	0.980^**
R+G+ B+L	-0.547^**	0.996^**	0.985^**	0.983^**	0.827^**	0.398^**	-0.517^**	-0.287	-0.353^*	-0.222	0.259	-0.010	0.983^**	0.989^**

指标 Index	TN	L	R	G	B	R/ （G+B）	L/ （R+G）	G/R	B/R	B/G	L/B	（R+B）/（R+B+G）	R+B	B+G
L	-0.575^**
R	-0.553^**	0.987^**
G	-0.597^**	0.991^**	0.960^**
B	-0.253	0.773^**	0.767^**	0.743^**
R/ （G+B）	-0.338^*	0.440^**	0.547^**	0.364^*	0.021
L/ （R+G）	0.473^**	-0.531^**	-0.585^**	-0.535^**	-0.209	-0.594^**
G/R	0.011	-0.268	-0.415^**	-0.145	-0.297	-0.755^**	0.344^*
B/R	0.508^**	-0.430^**	-0.460^**	-0.429^**	0.215	-0.813^**	0.618^**	0.234
B/G	0.503^**	-0.307^*	-0.267	-0.364^*	0.351^*	-0.455^**	0.460^**	-0.239	0.888^**
L/B	-0.460^**	0.346^*	0.337^*	0.373^*	-0.324^*	0.633^**	-0.464^**	0.024	-0.954^**	-0.964^**
R+B/ （R+B+G）	0.355^*	-0.082	0.025	-0.190	0.412^**	0.073	0.153	-0.708^**	0.521^**	0.855^**	-0.716^**
R+B	-0.473^**	0.964^**	0.971^**	0.934^**	0.899^**	0.382^*	-0.478^**	-0.395^**	-0.234	-0.051	0.108	0.172
B+G	-0.512^**	0.976^**	0.952^**	0.971^**	0.881^**	0.265	-0.452^**	-0.208	-0.226	-0.132	0.148	0.013	0.980^**
R+G+ B+L	-0.547^**	0.996^**	0.985^**	0.983^**	0.827^**	0.398^**	-0.517^**	-0.287	-0.353^*	-0.222	0.259	-0.010	0.983^**	0.989^**

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