Journal of Agricultural Science and Technology ›› 2023, Vol. 25 ›› Issue (5): 34-45.DOI: 10.13304/j.nykjdb.2022.0761
• BIOTECHNOLOGY & LIFE SCIENCE • Previous Articles Next Articles
Daokuan BAI(), Baojian GUO, Yi HONG, Mengna ZHANG, Juan ZHU, Chao LYU, Feifei WANG, Rugen XU(
)
Received:
2022-09-09
Accepted:
2022-12-15
Online:
2023-05-20
Published:
2023-07-13
Contact:
Rugen XU
白道宽(), 郭宝健, 洪益, 张萌娜, 朱娟, 吕超, 王菲菲, 许如根(
)
通讯作者:
许如根
作者简介:
白道宽 E-mail: b19711208b@163.com;
基金资助:
CLC Number:
Daokuan BAI, Baojian GUO, Yi HONG, Mengna ZHANG, Juan ZHU, Chao LYU, Feifei WANG, Rugen XU. Research on Mutagenic Mechanism and Genetic Mechanism of a Yellowing Mutant in Barley[J]. Journal of Agricultural Science and Technology, 2023, 25(5): 34-45.
白道宽, 郭宝健, 洪益, 张萌娜, 朱娟, 吕超, 王菲菲, 许如根. 一个大麦黄化突变体的突变机理及其遗传机制研究[J]. 中国农业科技导报, 2023, 25(5): 34-45.
基因名称 Gene name | 基因ID Gene ID | 正向引物 Forward primer(5’-3’) | 反向引物 Reverse primer(5’-3’) |
---|---|---|---|
HvCAO | HORVU.MOREX.r3.3HG0324440 | TGGTGCCCATAGATTGTTTC | GCCCTCATTCACTGAACCAAG |
COR | HORVU.MOREX.r3.2HG0191600 | TCTGCCTGCAAACCCCTCCTAG | TGTCCGTGGCATCCTGGACCTT |
PORB | HORVU.MOREX.r3.1HG0047060 | AGGCGTACAAGGACAGCAAGG | GTAGAGCGACGCGAAGGTGA |
PORA | HORVU.MOREX.r3.2HG0209470 | CCGTTCCAGAAGTTCGTCACC | CCTTGTTCCAGCTCCAGTACAC |
YCF3 | HORVU.MOREX.r3.5HG0423760 | GGTCTTTATCCTCGTCGTAT | TGTTCAATTTCTCGTGGAGT |
HvPTOX | HORVU.MOREX.r3.2HG0213170 | CTCTTAATCATGGAAGCGTTGG | TGGCGACAGTGACGAAGTAG |
MSH1 | HORVU.MOREX.r3.2HG0180240 | CCGAAGAAGAGTTCAGCAAT | TTCCATGCGAGGACATCACG |
HvCMF3 | HORVU.MOREX.r3.6HG0559110 | AATGCGAAATGCGAGGAGG | GGTCTTCGGTGCCTTTGTC |
GAPDH | HORVU.MOREX.r3.7HG0703580 | GTGAGGCTGGTGCTGATTACG | TGGTGCAGCTAGCATTTGAGA |
α-tublin | HORVU.MOREX.r3.1HG0082050 | AGTGTCCTGTCCACCCACTC | AGCATGAAGTGGATCCTTGG |
Table 1 Primer of chlorophyll synthesis, photosynthesis related genes used fou quantitative real-time PCR
基因名称 Gene name | 基因ID Gene ID | 正向引物 Forward primer(5’-3’) | 反向引物 Reverse primer(5’-3’) |
---|---|---|---|
HvCAO | HORVU.MOREX.r3.3HG0324440 | TGGTGCCCATAGATTGTTTC | GCCCTCATTCACTGAACCAAG |
COR | HORVU.MOREX.r3.2HG0191600 | TCTGCCTGCAAACCCCTCCTAG | TGTCCGTGGCATCCTGGACCTT |
PORB | HORVU.MOREX.r3.1HG0047060 | AGGCGTACAAGGACAGCAAGG | GTAGAGCGACGCGAAGGTGA |
PORA | HORVU.MOREX.r3.2HG0209470 | CCGTTCCAGAAGTTCGTCACC | CCTTGTTCCAGCTCCAGTACAC |
YCF3 | HORVU.MOREX.r3.5HG0423760 | GGTCTTTATCCTCGTCGTAT | TGTTCAATTTCTCGTGGAGT |
HvPTOX | HORVU.MOREX.r3.2HG0213170 | CTCTTAATCATGGAAGCGTTGG | TGGCGACAGTGACGAAGTAG |
MSH1 | HORVU.MOREX.r3.2HG0180240 | CCGAAGAAGAGTTCAGCAAT | TTCCATGCGAGGACATCACG |
HvCMF3 | HORVU.MOREX.r3.6HG0559110 | AATGCGAAATGCGAGGAGG | GGTCTTCGGTGCCTTTGTC |
GAPDH | HORVU.MOREX.r3.7HG0703580 | GTGAGGCTGGTGCTGATTACG | TGGTGCAGCTAGCATTTGAGA |
α-tublin | HORVU.MOREX.r3.1HG0082050 | AGTGTCCTGTCCACCCACTC | AGCATGAAGTGGATCCTTGG |
Fig. 1 Phenotypes of ‘YNP5’ and G039 under natural conditionA: Plant at seedling period; B: Extracts of photosynthetic pigments in the seedling period; C: Plant at filling period; D: Grain length; E: Grain width
Fig. 2 Contents of chlorophyll pigments in ‘YNP5’ and G039A: Seedling stage; B: Grain filling stage. ** means significantly differences between materials at P<0.01 level
性状 Trait | 扬农啤5号 YNP5 | G039 |
---|---|---|
株高 Plant height/cm | 86.46±5.94 | 72.86±5.42** |
穗下节间长 Internode length below the spike/cm | 30.88±3.36 | 22.38±2.40** |
分蘖数 Number of tillers | 17.82±8.36 | 8.85±3.40** |
穗长 Panicle length/cm | 6.71±0.48 | 5.95±0.66** |
每穗粒数 Grains per panicle | 28.35±2.42 | 25.86±2.23** |
千粒重 1 000-grain weight/g | 41.93±0.34 | 48.04±0.36** |
粒长 Grain length/mm | 6.86±0.03 | 7.62±0.03** |
粒宽 Grain width/mm | 3.05±0.02 | 3.15±0.02** |
Table 2 Agronomic traits of the wild type ‘YNP5’ and mutant G039
性状 Trait | 扬农啤5号 YNP5 | G039 |
---|---|---|
株高 Plant height/cm | 86.46±5.94 | 72.86±5.42** |
穗下节间长 Internode length below the spike/cm | 30.88±3.36 | 22.38±2.40** |
分蘖数 Number of tillers | 17.82±8.36 | 8.85±3.40** |
穗长 Panicle length/cm | 6.71±0.48 | 5.95±0.66** |
每穗粒数 Grains per panicle | 28.35±2.42 | 25.86±2.23** |
千粒重 1 000-grain weight/g | 41.93±0.34 | 48.04±0.36** |
粒长 Grain length/mm | 6.86±0.03 | 7.62±0.03** |
粒宽 Grain width/mm | 3.05±0.02 | 3.15±0.02** |
Fig. 3 SPAD values of the ‘YNP5’ and G039 under different temperature conditionsNote:** means significantly difference between materials at P<0.01 level.
Fig. 5 Chlorophyll content of the ‘YNP5’ and G039 under different temperature conditionsNote: ** means significantly difference between materials at P<0.01 level
Fig. 6 Photosynthetic indices of ‘YNP 5’ and G039 under different temperature conditionsA~E: Net photosynthetic rate, transpiration rate, stomatal conductance, water use efficiency and intercellular CO2 concentration are sequentially shown. * and ** mean significantly differences between materials at P<0.05 and P<0.01 levels,respectively
杂交组合 Cross combination | 植株数量 Number of plants | χ2 | P值(0.05) P value(0.05) | ||
---|---|---|---|---|---|
F2群体 F2 population | 野生表型 Wild type phenotype | 突变表型 Mutant phenotype | |||
G039/扬农啤5号 | 388 | 297 | 91 | 0.49 | 3.84 |
G039/扬农啤7号 | 332 | 252 | 80 | 0.14 | 3.84 |
Table 3 Genetic analysis of the mutant trait
杂交组合 Cross combination | 植株数量 Number of plants | χ2 | P值(0.05) P value(0.05) | ||
---|---|---|---|---|---|
F2群体 F2 population | 野生表型 Wild type phenotype | 突变表型 Mutant phenotype | |||
G039/扬农啤5号 | 388 | 297 | 91 | 0.49 | 3.84 |
G039/扬农啤7号 | 332 | 252 | 80 | 0.14 | 3.84 |
Fig. 8 Expression levels in genes involved in chlorophyll synthesis of YNP 5 and G039 under different temperature conditionsNote: ** means significant at P<0.01 level.
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